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研究生:楊貝宏
研究生(外文):Yann Nibéron
論文名稱:使用表面電漿共振技術檢測登革熱病毒
論文名稱(外文):Detection of Dengue virus using plasmonic resonance technique
指導教授:陳浩夫
指導教授(外文):How-Foo Chen
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:英文
論文頁數:110
中文關鍵詞:Applied Research and DevelopmentProfessional EquipmentOpticsEquipment
外文關鍵詞:Applied Research and DevelopmentProfessional EquipmentOpticsEquipment
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Dengue fever is a tropical disease caused by Dengue viruses infection (closely related to the viruses causing West Nile infection), which have four different subtypes. The virus is usually carried by A. aegypti, a type of mosquito. Dengue fever has become a major threat in tropical areas. Since 1960s, we estimate that, every year, 390 million dengue infections occur, and about 96 million of them result in illness. Surface plasmon resonance biosensor is well-known for its high sensitivity, its high resolution, real-time and label-free detection. This project focuses on the relevancy of using surface plasmon resonance biosensor to detect Dengue viruses.
Dengue fever is a tropical disease caused by Dengue viruses infection (closely related to the viruses causing West Nile infection), which have four different subtypes. The virus is usually carried by A. aegypti, a type of mosquito. Dengue fever has become a major threat in tropical areas. Since 1960s, we estimate that, every year, 390 million dengue infections occur, and about 96 million of them result in illness. Surface plasmon resonance biosensor is well-known for its high sensitivity, its high resolution, real-time and label-free detection. This project focuses on the relevancy of using surface plasmon resonance biosensor to detect Dengue viruses.
Acknowledgment 1
Overview 2
Introduction 3
Dengue virus 4
Surface Plasmon resonance 6
Plasmon theory 6
Application of the Surface Plasmon resonance to Bio-sensor 8
Simulation programs 9
Original surface plasmon resonance program 9
Multi-parameters programs 11
Treatment programs 15
The optical bio-sensor 17
Operating mode and composition 17
Optical set-up 17
Operative system 18
Set-up’s maintenance 21
Replacement of broken pieces 21
The alignment protocol 25
Device calibration 27
Parabolic mirror 27
Calibration technic 29
Surface treatment protocols 32
SF11 clean protocol 32
Gold coating protocols 33
Antibody coating protocol 33
Experimental Work 35
Stability test of the device 35
Sensitivity test of the device 40
Virus tests 41
Concentration:
Websites:
 WebMD. Dengue fever [on line]. Updated August 2015 [see February 2017]. Available on: http://www.webmd.com/a-to-z-guides/dengue-fever-reference#1
 Wikipedia. Dengue fever [on line]. Updated January 2017 [see February 2017]. Available on: https://en.wikipedia.org/wiki/Dengue_fever
 World Health Organization. Dengue and severe dengue [on line]. Updated July 2016 [see February 2017]. Available on: http://www.who.int/mediacentre/factsheets/fs117/en/
 BIOSENSING INSTRUMENT. BIOSENSING INSTRUMENT OFFERS INNOVATIVE SURFACE PLASMON RESONANCE INSTRUMENTS FOR THE COMMUNITY [on line]. Updated February 2017 [see February 2017]. Available on: http://biosensingusa.com/
Papers:
 World Health Organization. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition. Geneva: World Health Organization, 2009. 147 p. Laboratory diagnosis and diagnostic tests. ISBN-13: 978-92-4-154787-1.
 Chiu, N-F. Huang, T-Y. Lai, H-C and Aliofkhazraei, M. “Graphene Oxide Based Surface Plasmon Resonance Biosensors, Advances in Graphene Science”. InTech. 2013. DOI: 10.5772/56221
 Barchiesi, D. “A classroom theory of the surface plasmon polariton”. Eur. J. Phys. 33 (2012) 1345–1357. doi:10.1088/0143-0807/33/5/1345
 Chen, H-F. Lecture n°4: “guided mode SPR”, Dec 2016. National Yang-Ming University.
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