臺灣博碩士論文加值系統

台灣藜（Chenopodium formosanum Koidz.）為台灣原生種作物，傳統稱為紅藜，是台灣原住民耕作百年以上的農作物。近年來，在其高度營養價值被發掘之後，開始有民間業者大量栽種，成為新興的養生食品。本研究目的是以餵食混入台灣藜或含有台灣藜成分的銀寶膠囊之小鼠飼料餵養小鼠，探討台灣藜是否具有抗疲勞之功效。六週齡C57BL/6品系雄性小鼠分為五組：正常飼料組、低劑量銀寶膠囊組、中劑量銀寶膠囊組、高劑量銀寶膠囊組、中劑量台灣藜組，分別餵食2週後開始進行試驗。試驗方法分別為：強迫游泳試驗、血尿素氮試驗和血乳酸試驗。研究結果顯示餵食台灣藜以及銀寶膠囊的小鼠在強迫游泳試驗中，與對照組小鼠的游泳時間相比較，高劑量銀寶膠囊組達到顯著差異(p＜0.01)，低劑量銀寶膠囊組及中劑量組、中劑量台灣藜組則是達到極為顯著差異(p＜0.001)，顯示餵食中劑量台灣藜或不同劑量銀寶膠囊皆可以顯著提升小鼠強迫游泳的時間。在血尿素氮試驗中， 低劑量銀寶膠囊組與對照組沒有顯著差異；中劑量組及高劑量組銀寶膠囊組及中劑量台灣藜組則與對照組有極為顯著差異(p＜0.001)，顯示餵食中劑量(含)以上台灣藜或銀寶膠囊可以顯著降低小鼠游泳後血液中尿素氮的濃度。在血乳酸試驗中，低劑量及中劑量銀寶膠囊組小鼠游泳後的血乳酸上升值顯著比對照組小鼠降低(p＜0.05)，顯示低劑量及中劑量銀寶膠囊可以顯著降低小鼠游泳後的血乳酸濃度上升。高劑量銀寶膠囊組小鼠在游泳後的血乳酸上升值和休息後的乳酸下降值皆比對照組小鼠高，但是都沒有顯著差異；而中劑量台灣藜組小鼠的血乳酸上升值和下降值都比對照組小鼠低，但是也都沒有顯著差異。因此，本研究結論為餵食台灣藜以及銀寶膠囊皆能增加小鼠的強迫游泳時間，同時也可能在游泳後減少血液中尿素氮和乳酸的堆積，進而達到抗疲勞的功效，其中以中劑量銀寶膠囊的效果最為顯著。

關鍵字:台灣藜，抗疲勞，強迫游泳，血尿素氮，血乳酸。

Djulis (Chenopodium formosanum Koidz.) is a native crop in Taiwan. It has been called “Red pigweed” in Taiwan. It's a traditional crop by Taiwan aborigines cultivated for more than a century. In recent years, since its high nutritional value has been discovered, a large number of Djulis are now planted and it is developed to be a health food. The aim of this study is to investigate whether Djulis shows the anti-fatigue efficacy using a forced swimming mouse model. Mice were fed with fodder mixed with Djulis or a Djulis product, Djulis Yinbao Capsule (DYC). Six-week-old male C57BL/6 mice were divided into five groups: normal fodder fed group (control), fodder with low-dose DYC group, fodder with middle-dose DYC group, fodder with high-dose DYC group, and fodder with middle-dose Djulis group. After feeding for two weeks, mice in five different groups were examined their anti-fatigue efficacy by continuous swimming. The test methods are simplified by the methods for assessing the anti-fatigue efficacy of health foods announced by the Food and Drug Administration of the Ministry of Health and Welfare. They include forced swimming test, blood urea nitrogen test, and blood lactate test. Results of forced swimming test showed that mice fed with middle-dose Djulis and low- and middle-dose DYC showed significantly increasing times of swimming than mice in control group (p<0.001). Mice fed with high-dose DYC showed significantly increasing times of swimming than mice in control group (p<0.01). In brief, mice fed with fodder containing 3 different doses of DYC or middle-dose Djulis can all significantly elevate the forced swimming times than control mice. In blood urea nitrogen test, there was no significant difference between control and low-dose DYC groups, but showed significant differences in middle- and high-dose DYC and middle-dose Djulis groups when compared with control group (p<0.001). In brief, mice fed with fodder containing middle- or high-dose DYC or middle-dose Djulis can significantly reduce the concentrations of blood urea nitrogen after swimming. In the blood lactate test, the increments of blood lactate after swimming in both low- and middle-dose DYC were significantly lower than those in the control group (p<0.05), but there was no significant difference between high-dose DYC, middle-dose Djulis, and control groups. In brief, mice fed with fodder containing low- or middle-dose DYC can significantly reduce the concentrations of blood lactate after swimming. The reductions of blood lactate in mice after a 30-minute rest showed no significant difference between all tested group and control group. In summary, mice fed with fodder containing 3 different doses of DYC or middle-dose Djulis show anti-fatigue efficacy because of the increases of forced swimming times, reducing accumulations of blood urea nitrogen and lactate after swimming. Among all tested groups, middle dose of DYC is the most effective.

Key Words: Djulis, Anti-fatigue, Forced swimming test, Blood urea nitrogen, Blood lactate.

目錄
封面內頁
簽名頁
中文摘要iii
英文摘要v
誌謝vii
目錄viii
圖目錄xi
表目錄xii

1.前言1
2.文獻回顧2
2.1台灣藜2
2.1.1台灣藜介紹2
2.2台灣藜的營養成分2
2.3機能性成分3
2.3.1多酚類3
2.3.2甜菜色素4
2.4疲勞與抗疲勞4
2.5紅藜銀寶膠囊4
3.材料與方法6
3.1實驗材料與儀器6
3.1.1台灣藜6
3.1.2紅藜銀寶膠囊(銀寶膠囊)6
3.1.3實驗飼料6
3.1.4藥品6
3.1.5實驗小鼠、實驗分組與簡易實驗流程7
3.1.6儀器設備8
3.2實驗流程圖10
3.3實驗方法11
3.3.1實驗飼料的製備11
3.3.2強迫游泳試驗13
3.3.3血尿素氮試驗14
3.3.4血乳酸試驗14
3.4統計分析14
4.結果16
4.1台灣藜及銀寶膠囊對小鼠體重、飼料攝取量和飲水量的影響16
4.2台灣藜及其產品對小鼠強迫游泳試驗結果的影響27
4.3台灣藜及其產品對小鼠不負重游泳後血尿素氮試驗的影響30
4.4台灣藜及其產品對小鼠不負重游泳前、後血乳酸濃度的影響33
5.結論41
參考文獻42

圖目錄
圖1. 採血用具9
圖2. 實驗流程圖10
圖3. 試驗飼料製備過程12
圖4. 小鼠負重與模擬負重游泳的過程13
圖5. 各實驗組小鼠的體重變化量18
圖6. 各實驗組小鼠的體重增加量20
圖7. 各實驗組小鼠的飲食變化量21
圖8. 各實驗組小鼠的飲食量之差別23
圖9. 各實驗組小鼠的飲水變化量24
圖10. 各實驗組小鼠的飲水量之差別26
圖11. 各實驗組小鼠的強迫游泳時間28
圖12. 各實驗組小鼠的游泳後血尿素氮變化量31
圖13. 各實驗組小鼠的血乳酸變化量36
圖14. 各實驗組小鼠游泳後的乳酸上升量39
圖15. 各實驗組小鼠游泳休息後的乳酸下降量40

表目錄

表1. 紅藜銀寶膠囊的成分表5
表2. 各實驗組小鼠飼養前(體重)及兩周飼養後(體重)的體重19
表3. 各實驗組小鼠在飼養期間每三天的平均飲食量22
表4. 各實驗組小鼠在飼養期間每三天的平均飲水量25
表5. 各實驗組小鼠的平均負重游泳時間29
表6. 各實驗組小鼠游泳後的血尿素氮濃度32
表7. 各實驗組小鼠在游泳前、游泳後及休息後的血乳酸濃度(mM)37
表8. 各實驗組小鼠在游泳後的血乳酸上升濃度及休息後的血乳酸下降濃度(mM)38

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