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研究生:曹雁琪
研究生(外文):Yen-Chi Tsao
論文名稱:利用ISSR分子標誌分析球根花卉之遺傳歧異度
論文名稱(外文):Analysis of Genetic Diversity of Bulbous Flowers by ISSR Molecular Markers
指導教授:李佩芳李佩芳引用關係
指導教授(外文):Pei-Fang Lee
口試委員:張正朱木貴
口試委員(外文):Chen ChangMu-Kuei Chu
口試日期:2017-10-20
學位類別:碩士
校院名稱:輔英科技大學
系所名稱:生物科技系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2018
畢業學年度:106
語文別:中文
論文頁數:91
中文關鍵詞:金門原生百合野百合換錦花紅花石蒜玫瑰石蒜紅藍石蒜ISSR分子標誌
外文關鍵詞:Kinmen native lilyLilium brownii F. E. Brown ex MiellezLycoris sprengeriLycoris radiateLycoris × roseaLycoris × haywardii Traubinter simple sequence repeat (ISSR) markers
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誌謝Ⅰ
中文摘要Ⅱ
英文摘要 IV
目錄VI
表目錄VIII
圖目錄IX
縮寫表X
第一章 緒論1
第二章 文獻探討4
第一節 植物分類研究與應用4
一 形態學標記4
二 細胞學標記5
三 分子標記5
四 ISSR分子標誌技術應用9
第二節 百合研究概況11
一 百合分類11
二 野百合之分布鑑別13
三 金門原生百合鑑定14
四 野百合、臺灣百合、鐵炮百合形態特徵15
第三節 石蒜研究概況17
一 石蒜屬簡介17
二 石蒜屬分類19
三 紅藍石蒜、玫瑰石蒜、換錦花及紅花石蒜親緣關係23
四 馬祖東引鄉石蒜分布24
第三章 材料與方法25
第一節 材料25
第二節 方法27
一 葉片總DNA之萃取27
二 DNA定量28
三 ISSR分子標誌分析28
四 瓊脂膠體電泳分析PCR產物30
五 資料統計分析30
第四章 研究結果32
第一節 以ISSR分析金門原生百合親緣關係32
一 ISSR增幅條帶之引子32
二 多型性條帶統計分析32
三 遺傳相似度及群集分析樹狀圖33
第二節 以ISSR分析馬祖東引石蒜親緣關係56
一 ISSR增幅條帶之引子56
二 多型性條帶統計分析56
三 遺傳相似度及群集分析樹狀圖57
第五章 討論67
第一節 金門原生百合親緣關係分析67
第二節 石蒜親緣關係分析72
參考文獻77

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