臺灣博碩士論文加值系統

緹娜捕蟲堇(Pinguicula hybrida ‘Tina’)為狸藻科(Lentibulariaceae)的多年生地生草本植物，園藝應用上可用於盆花及溫室除蟲等用途。捕蟲堇傳統上以葉片扦插來繁殖，但小苗數少、生長緩慢且容易死亡。本研究探討以捕蟲堇葉片作為培植體，以2,4-D及TDZ進行葉片分化的測試，在得到足量的小芽後再以2,4-D及BA進行發根測試，之後再探討不同濃度的MS培養基對捕蟲堇小植株生長的影響，最後再測試馴化的介質。
捕蟲堇葉片的消毒方式以0.5%次氯酸鈉消毒3分鐘有較低的污染率及最高的分化率。將葉片培養於以1/2MS添加12mg/L TDZ有最多的小芽分化，平均每個培植體可以分化出約99.1個芽，而培養於不添加任何植物生長調節劑的培養基中所分化出的芽體長度是所有處理中最長的，約為0.83cm，而添加3mg/L 2,4-D及3mg/L TDZ的組別有最多綠色團粒狀堅硬的癒傷組織形成。待分化出的小芽長度約1cm時便開始進行發根的試驗，結果發現以不添加任何賀爾蒙的1/2MS有最佳約100%的發根率、平均發根數為10.3根以及最長約1.5cm的根長，而在添加植物生長調節劑後，開始出現芽體生長障礙且發根率及發根數都顯著下降，可能是因為捕蟲堇對植物生長調節劑相當敏感而導致的。當完整的小植株長高約2-3cm時，將其分別移入全量、1/2及1/3MS的培養基進行培養，結果發現其株高在第2週時略為上升後即明顯下降，而到了4週時才顯著回升；在全量MS培養基中可以得到最多的根，而培養於1/3MS在其基部有大量小芽的分化。所有的處理其馴化存活率皆達100%，發現除椰纖外其他三種處理，其株高皆會下降直到第四週時才會回升；以赤玉土及泥炭土馴化的處理其株寬皆穩定上升，而以水苔及椰纖馴化的組別其株寬則幾乎沒有增加；以水苔馴化者，其葉片數是穩定增加的，但其他處理的葉片數則沒有增加甚至是下降的，因此以泥炭土作為馴化的介質可以得到最大的植株。

Pinguicula hybrida ‘Tina’ (Lentibulariaceae) is a perennial herbaceous plants, it can be used in ornamental horticulture as pot flowers and in greenhouse as insectivorous plants. In tradition, it is reproduced by leaf insertion, but the reproductive rate is low, reproductive is slow, and its easily died. The purpose of this study is using the leaf of Pinguicula hybrida ‘Tina’ as explant, tested the effects of 2,4-D and TDZ in leaf culture, after getting sufficient shoots, then tested the effects of 2,4-D and BA on rooting, then tested the effects of different MS concentration on the plantlet growth, finally tested the planting disinfection medium fir plantlet domestication.
We found that using 0.5% NaClO for 3 minutes disinfection had the lowest contamination rate and the best differentiation rate of leaves. The leaves cultured on 1/2MS medium with 12mg/L TDZ has resulted in the most differentiation rate of shoot, in an average of 99.1. while cultures on PGRs free 1/2MS medium has the longest shoot length. it is about 0.83cm, the largest green granular hard callus was cultured on 1/2MS medium with 3mg/L 2,4-D and 3mg/L TDZ. After the shoot lengths were grew up to 1cm, the rooting test followed. Shoot cultured on PGRs free 1/2 MS has the highest rate of 100%, rooting, average 10.3 of roots number, and longest 1.5cm of root length. After add PGRs in medium, the shoot began grown disorderly, rooting rate and root number decreased significantly, this was probably because of Pinguicula hybrida ‘Tina’ was sensitive to PGRs. When the plantlet were growth about 2-3cm, then cultured on MS, 1/2MS, and 1/3MS for 4 weeks. We found the plant height were rise slightly and decrease significantly, and it will rise until fourth week. The plantlet cultured on MS medium had the most rooting number, and cultured on 1/3MS had differentiation the largest number of shoot. Every plantlet were survived in domestication, we found that every treatment except using coir as medium, their plant height were decline until the fourth week will revive, the plant width rise stable with using akadama soil and peat soil as domestication medium, and the plant width had hardly increase with another treatment. The leaves number were increase with using moss as medium, but the number of leaves in other treatment were decrease. Therefore, using peat soil as domestication of medium could get the largest plants.

摘要 I
ABSRACT II
誌謝 III
目錄 IV
表目錄 VI
圖目錄 VII
前言 1
一、研究動機 1
二、前人研究 1
(一)食蟲植物的簡介 1
(二)捕蟲堇的簡介 2
(三)食蟲植物的組織培養 3
(四)介質對組培苗馴化的影響 4
材料與方法 6
一、 植物材料 6
二、 試驗方法 6
(一)捕蟲堇之消毒方法 6
(二)捕蟲堇的葉片培養 6
(三)捕蟲堇的發根 6
(四)捕蟲堇小植株的生長 6
(五)捕蟲堇的馴化 7
三、 統計分析 7
結果 8
一、 捕蟲堇之消毒方法 8
二、 捕蟲堇的葉片培養 8
三、 捕蟲堇的發根 8
四、 捕蟲堇小植株的生長 9
五、 捕蟲堇的馴化 9
討論 11
一、 次氯酸鈉的濃度與消毒時間對無菌化存活率之探討 11
二、2,4-D及TDZ對捕蟲堇葉片誘導小芽及癒傷組織的影響 11
三、2,4-D及BA對捕蟲堇小芽發根及誘導癒傷組織的影響 12
四、不同濃度ＭＳ對捕蟲堇小植株生長的影響 13
五、不同介質對捕蟲堇小苗馴化及生長的影響 13
結論 15
參考文獻 16

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