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研究生:阮泓儒
研究生(外文):Juan, Hung-Ju
論文名稱:乾燥溫度、時間及基因型對番石榴葉片萃取物抗氧化能力與α-澱粉酶抑制活性的影響
論文名稱(外文):Effect of Drying Temperature, Duration and Genotype on Antioxidant Capacity and alpha-Amylase Inhibitory Activities of Guava Leaf Extract
指導教授:陳幼光
指導教授(外文):Chen, Yu-Kuang
口試委員:林慧玲林素汝
口試委員(外文):Lin, Huey-LingLin, Su-Ju
口試日期:2018-07-27
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2018
畢業學年度:106
語文別:中文
論文頁數:47
中文關鍵詞:總酚含量鐵還原抗氧化能力(FRAP)DPPH清除力
外文關鍵詞:total phenolic contentferric reducing antioxidant power (FRAP)DPPH scavenging power
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番石榴是台灣重要的經濟果樹之一。除了果實,其葉片富含抗氧化物質,具有預防退化性疾病的潛力。本研究的目的在了解乾燥溫度和時間對番石榴葉幼葉與成熟葉的總酚含量和抗氧化能力的影響,也探討基因型間總酚含量、抗氧化力和α-澱粉酶抑制率的變異。共分析了36個基因型,包括6個栽培種、5個引進或收集的品種(品系)、23個育種品系以及2個近緣種。結果顯示,在40℃下乾燥48小時幼葉通常在所有處理中具有最高的總酚含量和抗氧化能力,而在40℃的這些結果和處理與50℃乾燥12小時無顯著差異。葉片以50℃乾燥12小時,發現基因型間總酚含量、FRAP、DPPH清除力和α-澱粉酶抑制活性皆有顯著差異,而幼葉的總酚含量和抗氧化力優於成熟葉。基因型間幼葉的總酚含量、FRAP、DPPH清除能力和α-澱粉酶抑制率範圍分別為197.89至94.52 mg GAE / g、34.42至9.78 g Vitamin C / g、45.62至13.52 g Trolox / g和79.0%至13.7%。不論葉齡為何,總酚含量與FRAP和DPPH清除能力兩者均有高度顯著相關。從所分析的基因型中可篩選出總酚含量、抗氧化力和α-澱粉酶抑制率比常見的栽培種好的基因型,其可用於育種改良或保健產品的開發。
Guava is one of the important economic fruit trees in Taiwan. In addition to its fruits, its leaves are rich in antioxidant components and have the potential for the prevention of degenerative diseases. The aim of the present study was to understand the effects of drying temperature and duration on the total phenolic content and antioxidant capacity of guava leaves at young and mature stages. The variations of total phenolic content, antioxidant capacity and α-amylase inhibitory rate among genotypes were also investigated. In total, 36 genotypes were evaluated including six cultivars, five introduced or collected cultivars (line), 23 breeding lines and two related species. The results showed that drying young leaves at 40°C for 48 hours generally had the highest total phenolic content and antioxidant capacity among treatments. However, no significant difference was found between treatments at 40°C and at 50°C for 12 hours. When drying leaves at 50°C for 12 hours, it was found that there were significant variations in total phenolic content, FRAP, DPPH radical-scavenging power and α-amylase inhibitory activity among genotypes. Young leaves were better than mature leaves in terms of total phenolic content and antioxidant capacity. The total phenolic content, FRAP, DPPH scavenging power and α-amylase inhibition rate of the young leaves of the genotypes ranged from 197.89 to 94.52 mg GAE/g, 34.42 to 9.78 g Vitamin C/g, 45.62 to 13.52 g Trolox/g and 79.0% to 13.7%, respectively. Regardless of the leaf ages, total phenolic content is highly significantly correlated with both FRAP and DPPH scavenging power. Genotypes show better quality than the commonly grown cultivars in total phenolic content, antioxidant capacity and α-amylase inhibition rate have been selected. They can be used for further breeding or healthy product development.
目錄

摘要 I
Abstract II
謝誌 IV
目錄 V
圖表目錄 VIII
壹、前言 1
貳、前人研究 2
一、番石榴 2
(一)番石榴的簡介 2
(二)種類及特性 3
1. 泰國拔 3
2. 世紀拔 3
3. 梨仔拔 3
4. 西瓜拔 3
5. 珍珠拔 4
6. 草莓番石榴(P. cattleianum) 4
7. P. friedrichsthalianum 4
(三)番石榴的功效 4
1. 抗氧化性 4
2. 抗糖尿病與降血糖 5
3.抗菌 6
4.抗腹瀉 6
二、影響抗氧化力的因子 7
(一)品種 7
(二)部位 8
(三)葉齡 9
(四)萃取方式 9
三、澱粉酶(E.C.3.2.1.1) 10
(一) 介紹與分類 10
(二)澱粉酶與糖尿病的關係 10
參、材料與方法 13
一、植物材料 13
二、葉片乾燥與萃取 13
三、總酚含量分析 13
(一)試驗藥劑 13
(二)藥劑配製 14
(三)分析流程 14
四、FRAP 14
(一)試驗藥品 14
(二)藥品配製 14
(三)分析流程 15
五、DPPH清除力 15
(一)試驗藥品 15
(二)藥品配製 15
(三)分析流程 15
六、α-amylase抑制能力測定16
(一)試驗藥品 16
(二)藥品配置 16
(三)分析流程 16
七、統計分析 17
肆、結果 18
一、乾燥溫度與時間對番石榴葉片總酚含量與抗氧化能力的影響 18
二、基因型間幼葉與成熟葉總酚含量與抗氧化力的變異 18
三、幼葉與成熟葉之間總酚含量與抗氧化力的相關性 20
四、果實與葉片之間總酚含量與FRAP的相關性 20
五、基因型間幼葉的澱粉酶抑制率的變異以及其與總酚含量和抗氧化力的相關性 20
伍、討論 22
一、乾燥溫度與時間對總酚含量與抗氧化能力的影響 22
二、不同基因型與葉齡總酚含量與抗氧化能力的差異 22
三、番石榴葉的總酚含量、FRAP與DPPH清除力間的相關性 23
四、果實與葉片間總酚含量與FRAP的相關性 23
五、基因型間幼葉的澱粉酶抑制率 23
陸、結論 25
柒、參考文獻 39
作者簡介 47
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