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研究生:劉子綾
研究生(外文):Liou, Zih-Ling
論文名稱:美容中藥對斑馬魚幼魚及胚胎之影響:以桃仁、杏仁、薏仁為例
論文名稱(外文):The Effect of the Traditional Chinese Herbs for the Performance of Melanin on Zebrafish Embryo and Early Stage -Such as Persicae Semen, Armeniacae Amarum Semen and Coicis Semen
指導教授:陳麗文陳麗文引用關係
指導教授(外文):Chen, Li-Wen
口試委員:駱明潔林麗娟
口試委員(外文):Lo,Ming-JaeLin,Li-Jen
口試日期:2018-05-28
學位類別:碩士
校院名稱:國立臺中教育大學
系所名稱:科學教育與應用學系碩士班
學門:教育學門
學類:普通科目教育學類
論文種類:學術論文
論文出版年:2018
畢業學年度:106
語文別:中文
論文頁數:114
中文關鍵詞:黑色素桃仁杏仁薏仁斑馬魚
外文關鍵詞:melaninPersicae semenArmeniacae amarum semenCoicis semenzebrafish
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本研究旨在研究桃仁、杏仁、薏仁三味中藥在美容上的作用機轉,觀察野生種斑馬魚(zebrafish, AB)胚胎孵化率與死亡率,並研究其黑色素的表現情形。受精卵在受精後 0小時、12 小時、24 小時、36 小時、48 小時分別給予胚胎不同濃度之藥物劑量,並從藥物濃度反應及加藥時間點兩方面分別觀察發育指標。三味中藥的濃度選擇以相當人類 2倍使用劑量為最高劑量,桃仁的濃度為 1000ppm~4000ppm,杏仁的濃度1000ppm~4000ppm,薏仁則是 3000ppm~8000ppm。胚胎置於 24 孔盤中,每孔放 5 個,從受精後開始觀察,不論加藥與否,之後每 12 小時就觀察記錄一次,直至受精後 120小時止,若有存活的胚胎則會持續養至 1 個月,並觀察體表的黑色素表現情形。黑色素的表現是以體表色塊比例,在胚胎受精後 72 與 120 小時使用 imang J ,依蕭崇德教授的方法―斑馬魚黑色素偵測法,換算其比例,視為黑色素表現情形。
研究結果顯示:(1)加藥濃度與加藥時間點會對斑馬魚胚胎產生交互影響(2)桃仁在 12小時加藥濃度 1000ppm,不會影響胚胎孵化率與死亡率,而在第 120 小黑色素變化量有顯著下降(3)杏仁在班馬魚黑色素淡化時,會有孵化率下降及死亡率上升的現象(4)薏仁在 0 小時加藥濃度 5000ppm;在 0 小時、24 小時與 36 小時加藥濃度 5000ppm;在 36小時加藥濃度 6000ppm;在 48 小時加藥濃度 7000ppm 等組別,不會影響其胚胎孵化率與死亡率,而黑色素變化量在第 72 與 120 小時觀察有顯著下降。本研究證實桃仁、杏仁、薏仁確實有淡化黑色素之效果,但建議要在上述時間與藥物劑量下使用,以免產生生理危害,並達到最佳美白效果。
To reduce the side effect of chemical compounds in human body, traditional Chinese herbs for whitening is popular in nowadays. Persicae semen、Armeniacae amarum semen and Coicis semen are in this category. The purpose of this study was compared the effects of three traditional Chinese herbs-Persicae semen、Armeniacae amarum semen and Coicis semen on the development of zebrafish melanin. We used zebrafish (pronounced star-AB) from Taiwan Zebrafish Core Facility at Academia Sinica as our animal model. Four developmental stages (zygote, segmentation, pharyngula, hatching) were selected to add drugs. The hatching rate, the mortality rate and the performance of melanin were measured in different concentration and time to add drug. It was shown that: (1)The time of added drug in zebrafish interacted with the concentration of added drug in zebrafish.(2)When added drug at twelfth hour, the melanin rate was a significant difference between control and the drugs of Persicae semen was added 1000ppm,and the hatching rate、mortality rate were a nonsignificant difference between control and the drugs of Persicae semen was added 1000ppm.(3) When added drug at 0 hour and twenty-fourth hour, the melanin rate was a significant difference between control and the drugs of Coicis semen was added 5000ppm,and the hatching rate、mortality rate were a nonsignificant difference between control and the drugs of Coicis semen was added 5000ppm.
目錄
摘要 I
Abstract II
目錄 III
表目次 IV
圖目次 V
第一章 緒論 1
1.1 研究背景與動機 1
1.2 研究目的 3
第二章 文獻探討 4
2.1 黑色素與黑色素淡化機制 4
2.2 美容中藥 10
2.3 斑馬魚 16
第三章 實驗材料與方法 23
3.1 動物準備 25
3.2 藥品配置 27
3.3 實驗設計 30
第四章 實驗結果 32
4.1桃仁對斑馬魚胚胎的影響 32
4.2 杏仁對斑馬魚胚胎的影響 50
4.3 薏仁對斑馬魚胚胎的影響 69
第五章 討論與結論 90
5.1 本研究之討論 90
5.2 本研究之未來建議 93
5.3 本研究之結論 93
參考文獻 94


參考文獻
中華民國行政院環境保護署環境檢驗所主編(2017)。 生物急毒性檢測方法-斑馬魚胚胎半靜水式法。 中華民國行政院環境保護署環境檢驗所。
王思涓(2002)。 薏苡籽實中特殊生理機能性成分的定量分析與比較。 國立臺灣大學食品科學研究所碩士論文。
王玲波、徐文東(2014)。 苦杏仁與甜杏仁的鑑別研究。 黑龍江醫藥, 27(2), 278-280。
台灣營養食品資料庫(2017)。 取自https://consumer.fda.gov.tw/Food/TFND.aspx?nodeID=178
余佳紋,巫松泉,易光輝(2008)。 甲氧基桂皮酸辛酯對α及β熊果素穩定性之影響。弘光學報, 52, 297-314。
吳蕙如(2013)。 以斑馬魚胚胎評估半導體廢水之生物毒性。 國立交通大學環境工程研究所碩士論文。
周柏肇(2015)。 活血化瘀中藥對斑馬魚胚胎的影響-以三稜、莪朮、水蛭為例。 國立台中教育大學科學教育與應用學系研究所碩士論文。
林志虎(2008)。含七白膏複方萃取物化妝品之抗老化與美白有效性評估。 國立臺灣海洋大學食品科學研究所碩士論文。
林敬鈞(2010)。 利用斑馬魚篩選抗黑色素之中草藥。 國立台南大學生物科技研究所碩士論文。
范元鵠(2006)。川芎、細辛、白朮及杏仁萃取物之美白與抗老化效果與含此等萃取物之化妝品的有效性。 國立台灣海洋大學食品科學研究所碩士論文。
張金艷、何萍、李貽奎(2010)。 苦杏仁、桔梗及二者配伍止咳祛痰作用的研究。 中國實驗方劑學雜誌, 18(2), 173-175。
張惠淇(2002)。 中藥美白化妝品其安全品質與療效之評估。 中國醫藥學院中國藥學研究所碩士論文。
張岱琪(2008)。 銅離子對細胞發育的影響-以斑馬魚色素生成為指標。 中山醫學大學醫學檢驗暨生物技術學系研究報告。
陳玉芬、張乃方、吳珮瑄(2014)。 化妝品調製學(第四版)。 台中:華格那企業。
陳雨音(2008)。 薏仁對改善中老年人血糖、血脂及老人斑之影響。 國立臺灣海洋大學食品科學研究所碩士論文。
陳裕星、張嘉倫、廖宜倫、林雲康(2014)。 不同品種及產地薏苡籽實之化學指紋圖譜建立。 臺中區農業改良場研究彙報, 124(2), 1-16。
湯慶發、謝穎、陳飛龍、郭陽、宋帥(2013)。 苦杏仁中苦杏仁苷的存在形式及其影響因素。 中國實驗方劑學雜誌, 13(8), 107-109。
黃士禮、陳瑤峰、江文章(1994)。 省產薏苡籽實中氨基酸、脂肪酸和一般組成分分析。 食品科學, 21(1), 67-74。
楊爽、王李梅、王姝麒、郭曉江、任冬梅(2011)。 薏苡化學成分及其活性综述。 中藥材, 38(8), 23-28。
熊皓儀(2014)。 核桃仁乙酸乙酯區分物及純化物質之抗氧化及醣解酵素醣解酵素抑制作用。 東海大學食品科學研究所碩士論文。
趙永見、牛凱、唐德志、梁倩倩、舒冰、李晨光、 …鄭為超(2015)。 桃仁藥理作用研究近況。 遼寧中醫雜誌, 42(4), 888-890。
蔡敬民、楊莉君、許輝吉(1999)。 薏苡攝取可改善倉鼠因高油飲食所造成之肝臟脂質堆積。 食品科學, 26(3),265-276。
羅怡情(2010)。 化妝品成分辭典(第六版)。 聯經出版股份事業有限公司。
Abd, E., Yousef, S. A., Pastore, M. N., Telaprolu, K., Mohammed, Y. H., Namjoshi, S., & Roberts, M. S. (2016). Skin models for the testing of transdermal drugs. Clinical pharmacology: advances and applications, 8, 163-170.
Balaguer, A., Chisvert, A., & Salvador, A. (2008). Environmentally friendly LC for the simultaneous determination of ascorbic acid and its derivatives in skin‐whitening cosmetics. Journal of Separation Science, 31(2), 229-236.
Becker Jr, S. W., & Zimmermann, A. A. (1955). Further studies on melanocytes and melanogenesis in the human fetus and newborn. The Journal of Investigative Dermatology, 25(2), 103-112.
Briganti, S., Camera, E., & Picardo, M. (2003). Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell & Melanoma Research, 16(2), 101-110.
Biniek, K., Levi, K., & Dauskardt, R. H. (2012). Solar UV radiation reduces the barrier function of human skin. Proceedings of the National Academy of Sciences, 109(42), 17111-17116.
Chang, T. S. (2009). An updated review of tyrosinase inhibitors. International
journal of molecular sciences, 10(6), 2440-2475.
Carvalho, M., Ferreira, P. J., Mendes, V. S., Silva, R., Pereira, J. A., Jerónimo, C., & Silva, B. M. (2010). Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chemical Toxicology, 48(1), 441-447.
Calcabrini, C., De Bellis, R., Mancini, U., Cucchiarini, L., Stocchi, V., & Potenza, L. (2017). Protective effect of Juglans regia L. walnut extract against oxidative DNA damage. Plant Foods for Human Nutrition, 72(2), 192-197.
Čolić, S. D., Akšić, M. M. F., Lazarević, K. B., Zec, G. N., Gašić, U. M., & Natić, M. M. (2017). Fatty acid and phenolic profiles of almond grown in Serbia. Food Chemistry, 234(1), 455-463.
Choi, T. Y., Kim, J. H., Ko, D. H., Kim, C. H., Hwang, J. S., Ahn, S., & Yoon, T. J. (2007). Zebrafish as a new model for phenotype‐based screening of melanogenic regulatory compounds. Pigment Cell & Melanoma Research, 20(2), 120-127.
Camp, E., & Lardelli, M. (2001). Tyrosinase gene expression in zebrafish embryos. Development Genes & Evolution, 211(3), 150-153.
Desmedt, B., Courselle, P., De-Beer, J. O., Rogiers, V., & De Paepe, K. (2016). Overview of skin whitening agents with an insight into the illegal cosmetic market in Europe. Journal of the European Academy of Dermatology and Venereology, 30(6), 943-950.
Fuchs, E. (2007). Scratching the surface of skin development. Nature, 445 (7130), 834-842.
Fraser, T. W., Khezri, A., Lewandowska-Sabat, A. M., Henry, T., & Ropstad, E. (2017). Endocrine disruptors affect larval zebrafish behavior: Testing potential mechanisms and comparisons of behavioral sensitivity to alternative biomarkers. Aquatic Toxicology, 193(10), 128-135.
Fu, X. J., Song, X. X., Wei, L. B., & Wang, Z. G. (2013). Study of the distribution patterns of the constituent herbs in classical Chinese medicine prescriptions treating respiratory disease by data mining methods. Chinese journal of integrative medicine, 19(8), 621-628.
Ghosh, C., & Collodi, P. (1994). Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos. Cytotechnology, 14(1), 21-26.
Hung, M. W., Zhang, Z. J., Li, S., Lei, B., Yuan, S., Cui, G. Z.,…Lee, S. M. Y. (2012). From omics to drug metabolism and high content screen of natural product in zebrafish: a new model for discovery of neuroactive compound. Evidence-Based Complementary and Alternative Medicine, 20(12), 20.
Henning, F., Jones, J. C., Franchini, P., & Meyer, A. (2013). Transcriptomics of morphological color change in polychromatic Midas cichlids. Bmc Genomics, 14(1), 171.
Kinsler, V. A., Polubothu, S., Calonje, J. E., Chong, W. K., & Morrogh, D. (2017). Copy number abnormalities in new or progressive ‘neurocutaneous melanosis’ confirm it to be primary CNS melanoma. Acta neuropathologica, 133(2), 329-331.
Kim, K. N., Yang, H. M., Kang, S. M., Ahn, G. N., Roh, S. W., Lee, W.,...Jeon, Y. J. (2015). Whitening effect of octaphlorethol A isolated from Ishige foliacea in an in vivo zebrafish model. J. Microbiol. Biotechnol, 25(4), 448-451.
Kneipp, M., Estrada, H., Lauri, A., Turner, J., & Razansky, D. (2015). Volumetric tracking of migratory melanophores during zebrafish development by optoacoustic microscopy. Mechanisms of Development, 13(8), 300-304.
Kameyama, K., Sakai, C., Kondoh, S., Yonemoto, K., Nishiyama, S., Tagawa, M., & Bucks, D. (1996). Inhibitory effect of magnesium L-ascorbyl-2-phosphate (VC-PMG) on melanogenesis in vitro and in vivo. Journal of the American Academy of Dermatology, 34(1), 29-33.
Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B., & Schilling, T. F. (1995). Stages of embryonic development of the zebrafish. Developmental dynamics, 203(3), 253-310. doi:10.1002/aja.1002030302.
Kochevar, I. E., Moran, M., Lyon, N., Flotte, T., Siebert, E., & Gange, W. (1993). Effects of systemic indomethacin, meclizine and BW755C on chronic ultraviolet B-induced effects in hairless mouse skin. The Society for Investigative Dermatology, 2(4), 186-193.
Li, K., Yang, W., Li, Z., Jia, W., Li, J., Zhang, P., & Xiao, T. (2016). Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes. International immunopharmacology, 34(3), 189-198.
Li, G., Ye, H., Su, G., Han, Z., Xie, C., Zhou, B., & Liu, C. (2017). Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae. Chemosphere, 18(6), 209-217.
Lammer, E., Carr, G. J., Wendler, K., Rawlings, J. M., Belanger, S. E., & Braunbeck, T. (2009). Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test? Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(2), 196-209.
Logan, D. W., Bryson-richardson, R. J., Taylor, M. S., Currie, P., & Jackson, I. J. (2003). Sequence characterization of teleost fish melanocortin receptors. Annals of the New York Academy of Sciences, 994(1), 319-330.
Mericli, F., Becer, E., Kabadayı, H., Hanoglu, A., Yigit Hanoglu, D., Ozkum Yavuz, D., & Vatansever, S. (2017). Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil. Pharmaceutical biology, 55(1), 1239-1248.
Maj, J., Jankowska-Konsur, A., Gruber, J., Woźniak, Z., & Hryncewicz-Gwóźdź, A. (2017). Diffuse melanosis cutis related to dermal micrometastases as the first clinical symptom of distant metastatic malignant melanoma: Case report. Medicine, 96(15), 647-651.
Ortiz-Ruiz, C. V., Berna, J., Tudela, J., Varon, R., & Garcia-Canovas, F. (2016). Action of ellagic acid on the melanin biosynthesis pathway. Journal of Dermatological Science, 82(2), 115-122.
Parvez, S., Kang, M., Chung, H. S., Cho, C., & Bae, H. (2006). Survey and mechanism of skin depigmenting and lightening agents. Phytotherapy Research, 20(11), 921-934.
Picardo, M., & Slominski, A. T. (2017). Melanin pigmentation and melanoma. Experimental Dermatology, 26(7), 555-556.
Raja, V., Ahmad, S. I., Irshad, M., Wani, W. A., & Shreaz, S. (2017). Anticandidal activity of ethanolic root extract of Juglans regia (L.): Effect on growth, cell morphology, and key virulence factors. Journal de Mycologie Médicale/Journal of Medical Mycology, 33(16), 162-167.
Slominski, A., & Wortsman, J. (2000). Neuroendocrinology of the skin. Endocrine Reviews, 21(5), 457-487.
Seiji, M., & Fitzpatrichk, T. B. (1961). The reciprocal relationship between melanization and tyrosinase activity in melanosomes (melanin granules). The Journal of Biochemistry, 49(6), 700-706.
Saghaie, L., Pourfarzam, M., Fassihi, A., & Sartippour, B. (2013). Synthesis and tyrosinase inhibitory properties of some novel derivatives of kojic acid. Research in Pharmaceutical Sciences, 8(4), 233-239.
Tada, M., Kohno, M., & Niwano, Y. (2014). Alleviation effect of arbutin on oxidative stress generated through tyrosinase reaction with L-tyrosine and L-DOPA. BMC Biochemistry, 15(1), 23-27.
Tamura, T., Mitsumori, K., Totsuka, Y., Wakabayashi, K., Kido, R., Kasai, H., & Hirose, M. (2006). Absence of in vivo genotoxic potential and tumor initiation activity of kojic acid in the rat thyroid. Toxicology, 222(3), 213-224.
Tsetskhladze, Z. R., Canfield, V. A., Ang, K. C., Wentzel, S. M., Reid, K. P., Berg, A. S.,…Cheng, K. C. (2012). Functional assessment of human coding mutations affecting skin pigmentation using zebrafish. PloS one, 7(10), 47-53. doi:10.1371/journal.pone.0047398
Verstraelen, S., Peers, B., Maho, W., Hollanders, K., Remy, S., Berckmans, P., & Witters, H. (2016). Phenotypic and biomarker evaluation of zebrafish larvae as an alternative model to predict mammalian hepatotoxicity. Journal of Applied Toxicology, 36(9), 1194-1206.
Wang, S., Liu, X. M., Zhang, J., & Zhang, Y. Q. (2014). An efficient preparation of mulberroside A from the branch bark of mulberry and its effect on the inhibition of tyrosinase activity. PLoS One, 9(10), 109-116.
Weiss-Errico, M. J., Berry, J. P., & O’Shea, K. E. (2017). β-Cyclodextrin Attenuates Perfluorooctanoic Acid Toxicity in the Zebrafish Embryo Model. Toxics, 5(4), 31. doi:10.3390/toxics5040031
Xu, Q., Bauer, R., Hendry, B. M., Fan, T. P., Zhao, Z., Duez, P., & Hylands, P. J. (2013). The quest for modernisation of traditional Chinese medicine. BMC Complement Altern Med, 13(1), 132-142.
Yang, J., Liu, R. H., & Halim, L. (2009). Antioxidant and antiproliferative activities of common edible nut seeds. LWT-Food Science and Technology, 42(1), 1-8.
Yang, X., Lin, J., Peng, X., Zhang, Q., Zhang, Y., Guo, N., & Li, Q. (2017). Effects of picrotoxin on zebrafish larvae behaviors: A comparison study with PTZ. Epilepsy & Behavior, 70(5), 224-231.
Yang, L., Ho, N. Y., Alshut, R., Legradi, J., Weiss, C., Reischl, M., & Strähle, U. (2009). Zebrafish embryos as models for embryotoxic and teratological effects of chemicals. Reproductive Toxicology, 28(2), 245-253.
Zhang, C., Wang, J., Dong, M., Wang, J., Du, Z., Li, B., & Zhu, L. (2017). Effect of 1-methyl-3-hexylimidazolium bromide on zebrafish (Danio rerio). Chemosphere, 192(6), 348-353.

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