跳到主要內容

臺灣博碩士論文加值系統

(44.200.194.255) 您好!臺灣時間:2024/07/19 06:29
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:湯期文
研究生(外文):Qi-Wen Tang
論文名稱:雙光子聚合技術應用於大範圍之產品設計與製造
論文名稱(外文):Design and Fabrication of Large Scale 3D Objects by Two-Photon Polymerization Technology
指導教授:鍾添東鍾添東引用關係
口試委員:王安邦許聿翔
口試日期:2017-07-31
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:機械工程學研究所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2017
畢業學年度:106
語文別:英文
論文頁數:88
中文關鍵詞:雙光子聚合雙光子吸收飛秒雷射振鏡掃描系統疏水性結構細胞遷移表面粗糙度
相關次數:
  • 被引用被引用:0
  • 點閱點閱:113
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本文研究以雙光子聚合技術應用於大尺寸三維產品的設計與製造。本雙光子聚合製造系統使用130 kHz高重複率之Nd:YAG雷射、三維壓電平台,並搭配放大倍率50倍、數值孔徑0.8的物鏡製作細胞培養基質與疏水性結構。藉由直接聚焦雷射於樹脂而不通過玻片,再以蜂巢式製作方法避免結構倒塌,製作出具有曲面結構的細胞培養基質以及具有特定表面粗糙度的細胞培養基質,予以提供造骨細胞遷徙之相關研究。再者,本研究根據Cassie & Baxter模型,製作出不同設計參數的疏水結構並量測其接觸角,將其疏水結構製作於PDMS晶片之微流道中以進行二項流產生測試。比較兩者有無加入疏水性結構之流態分佈圖,可發現疏水性結構可有效減小環狀流之面積。最後,本研究建構了一組大範圍雙光子製造系統,其包含4瓦高功率飛秒雷射、振鏡掃描系統,壓電驅動平台以及傾斜校正平台。藉由整合以上元件,可製作邊長1毫米的方形光柵以及直徑3毫米的台灣大學校徽。
This thesis studies on design and fabrication of large scale 3D objects by two-photon polymerization. The established TPP fabrication system equipped with 130 kHz repetition rate Nd: YAG laser, 3D piezostage, and 50x microscope objective with NA=0.8 were used to fabricate substrates for cell migration and hydrophobic structures. With laser beam directly focused on resin without through coverslip and honeycomb bulkhead method for avoiding structures collapse, the substrates with curvature surface and the substrates with different surface roughness regions were fabricated and provided for studying the migrating behavior of human osteosarcoma cells. Moreover, according to the Cassie & Baxter model, hydrophobic structures with different design parameters were fabricated. The hydrophobic structure with droplets contact angle greater than 90 degree is made inside the channel of PDMS chip for emulsion generation. Comparing the flow regimes map of PDMS chip with and without hydrophobic structures, the annular flow region becomes narrower with hydrophobic structure. In addition, a large scale TPP fabrication system including 4W high power femtosecond laser, galvanometer scanner, piezo-driven translation stage and tilt stage was established. With the integration of these components, large-scale optical grating with size of 1mm x 1mm and the logo of National Taiwan University with 3mm diameter can be manufactured successfully.
口試委員審定書 i
致謝 ii
摘要 iii
Abstract iv
CONTENTS v
LIST OF FIGURES vii
LIST OF TABLE xii
NOMENCLATURES xiii
Chapter 1 Introduction 1
1.1 Background 1
1.2 Literature Review 3
1.3 Research Motivation 11
1.4 Thesis outline 12
Chapter 2 Principle and fabrication process of TPP 13
2.1 Fundamental principle of TPP process 13
2.2 The fabrication process of TPP 17
2.3 NTUMFS CAM system for TPP micro fabrication 18
2.4 Experimental setup of TPP micro fabrication based on 3D piezostage 19
2.5 Supercritical point drying process 21
2.6 Scanning electronic microscope image 24
Chapter 3 Large scale TPP system based on galvanometer scanner 25
3.1 Hardware development of large scale TPP system 25
3.2 Software design for large scale TPP system 30
3.3 2D & 3D fabrication with large scale TPP system 34
Chapter 4 Design and fabrication of substrates for cell migrating with TPP 40
4.1 Experimental setup for fabrication of substrates 40
4.2 Fabrication strategy of substrates with different curvature on surface 46
4.3 Fabrication of substrates with specific roughness 50
Chapter 5 Design and fabrication of hydrophobic structure with TPP 59
5.1 Design of hydrophobic structure 59
5.2 Fabrication and measurement of hydrophobic structure 61
5.3 Application of hydrophobic structure in PDMS chips for emulsion generation 66
Chapter 6 Conclusions and Suggestions 73
6.1 Conclusions 73
6.2 Suggestions 74
References 76
Appendix A: User manual of large scale TPP system 82
A.1 Program setting 82
A.2 Hardware Installation 87
Vitae 88
[1]D. Tan, Y. Li, F. Qi, H. Yang, and Q. G. Dong and X. Duan, "Reduction in feature size of two-photon polymerization using SCR500," Applied Physics Letters, vol. 90, no. 7, 2007.
[2]M. Power, G. Z. Yang, "Direct laser written passive micromanipulator end-effector for compliant object manipulation," in IEEE/RSJ International Conference on Intelligent Robots and Systems, pp.790-797, 2015.
[3]N. Bertin, T. Spelman, O. Stephan, L. Gredy, M. Bouriau, E. Lauga, P. Marmottant, "Propulsion of Bubble-Based Acoustic Microswimmers," Physical Review Applied, vol. 4, no. 6, p. 064012, 2015.
[4]P. S. Timashev, M. V. Vedunova, D. Guseva, E. Ponimaskin, A. Deiwick, T. A. Mishchenko, E. V. Mitroshina, A. V. Koroleva, A. S. Pimashkin, I. V. Mukhina, V. Ya. Panchenko, B. N. Chichkov and V. N. Bagratashvili, "3D in vitroplatform produced by two-photon polymerization for the analysis of neural network formation and function," Biomedical Physics & Engineering Express, vol. 2, no. 3, p. 035001, 2016.
[5]T. StichelEmail, B. Hecht, R. Houbertz, G. Sextl, "Compensation of spherical aberration influences for two-photon polymerization patterning of large 3D scaffolds," Applied Physics A, vol. 121, no. 1, pp. 187-191, 2015.
[6]I. Wang, M. Bouriau, P. L. Baldeck, C. Martineau, and C. Andraud, "Three-dimensional microfabrication by two-photon-initiated polymerization with a low-cost microlaser," Optics Letters, vol. 27, no. 15, pp. 1348-1350, 2002.
[7]M. Göppert‐Mayer, "Über Elementarakte mit zwei Quantensprüngen," Annalen der Physik, vol. 401, no. 3, pp. 273-294, 1931.
[8]W. Kaiser, and C.G.B. Garrett, "Two-Photon Excitation in CaF2:Eu2+," Physical Review Letters,vol. 7, no. 6, pp. 229-231, 1961.
[9]K.-S. Lee, D.-Y. Yang, S. H. Park, R. H. Kim, "Recent developments in the use of two-photon polymerization in precise 2D and 3D microfabrications," Polymers for Advanced Technologies, vol. 17, no. 2, pp. 72-82, 2006.
[10]S. Maruo, and S. Kawata, "Two-Photon-Absorbed Near-Infrared Photopolymerization for the-dimensional microfabrication," Jouranl of microelectromechanical system, vol. 7, no. 4, pp. 411-415, 1998.
[11]H.C. Ishikawa-Ankerhold, R. Ankerhold, and G.P. Drummen, "Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM," Molecules, vol. 17, no. 4, pp. 4047-4132, 2012.
[12]S. Maruo, O. Nakamura, and S. Kawata, "Three-dimensional microfabrication with two-photon- absorbed photonpolymerization," OPTICS LETTERS, vol. 22, no 2, pp. 132-134, 1997.
[13]Robert J. DeVoe, Harvey W. Kalweit, Catherine A. Leatherdale, Todd R. Williams, "Voxel shapes in two-photon microfabrication," in SPIE Proceedings, pp. 310-316, 2003.
[14]H.-B. Sun, T. Tanaka, and S. Kawata, "Three-dimensional focal spots related to two-photon excitation," Applied Physics Letters, vol. 80, no. 20, pp. 3673-3675, 2002.
[15]M. Malinauskas, V. Purlys, M. Rutkauskas, R. Gadonas, "Two-photon polymerization for fabrication of three-dimensional micro- and nanostructures over a large area," in SPIE Proceedings, pp. 72040C-72040C-11, 2009.
[16]Kotaro Obata, Ayman El-Tamer, Lothar Koch, Ulf Hinze and Boris N Chichkov, "High-aspect 3D two-photon polymerization structuring with widened objective working range (WOW-2PP)," Light: Science & Applications, vol. 2, no. 12, p. e116, 2013.
[17]K. Cheng, X. Zhou, X. Zheng, J. Lin, "Study on the consistency of the voxel of two photon polymerization with inclined beam," Optics Communications, vol. 381, no. 15, pp. 444-449, 2016.
[18]G. Göring, P.-I. Dietrich, M. Blaicher, S. Sharma, J. G. Korvink, T. Schimmel, C. Koos, and H. Hölscher, "Tailored probes for atomic force microscopy fabricated by two-photon polymerization," Applied Physics Letters, vol. 109, no. 6, p. 063101, 2016.
[19]M. Bieda, F. Bouchard, and A.F. Lasagni, "Two-photon polymerization of a branched hollow fiber structure with predefined circular pores," Journal of Photochemistry and Photobiology A: Chemistry, vol. 319, p. 1-7, 2016.
[20]M. Payer, M. Lorenzoni, N. Jakse, R. Kirmeier, G. Dohr, M. Stopper, C. Pertl, "Cell growth on different zirconia and titanium surface textures: a morphologic in vitro study," J Dental Implant, pp. 338–351, 2010.
[21]G. Zhao, Z. Schwartz, M. Wieland, F. Rupp, J. Geis-Gerstorfer, D. L. Cochran, B. D. Boyan, "High surface energy enhances cell response to titanium substrate microstructure," Journal of Biomedical Materials Research, vol. 74A, no. 1, pp. 49-58, 2005.
[22]S. H. Park, T. W. Lim, D.-Y. Yang, S. W. Yi, H. J. Kong, "Direct Fabrication of Micropatterns and Three-Dimensional Structures Using Nanoreplication-Printing (nRP) Process. Sensors and Materials," vol. 17, no. 2, pp. 65-75, 2005.
[23]M.J. Nasse, and J.C. Woehl, "Realistic modeling of the illumination point spread function in confocal scanning optical microscopy," Journal of the Optical Society of America A, vol. 27, no, 2, pp. 295-302, 2010.
[24]H.-B. Sun, and S. Kawata, "Two-photon laser precision microfabrication and its applications to micro-nano devices and systems," Journal of Lightwave Technology, vol. 21, no. 3, pp. 624-633, 2003.
[25]C.-Y. Liao, "Product Model Acquisition, Preparation, and Simulation for Two-Photon Polymerization Micro-manufacturing," Joint Ph.D. dissertation, University of Joseph Fourier and National Taiwan University, France and Taiwan, 2008.
[26]D.S. Correa, Leonardo De Boni, A.J.G. Otuka, Vinicius Tribuzi, C.R. Mendonça, "Two-Photon Polymerization Fabrication of Doped Microstructures," in Polymerization, A.D.S. Gomes, Editor, InTech: Rijeka. Ch. 15, 2012.
[27]T. Hasegawa, and S. Maruo, "Two-photon microfabrication with a supercritical CO2 drying process toward replication of three-dimensional microstructures," in International Symposium on Micro-NanoMechatronics and Human Science, pp. 12-15, 2007.
[28]S. Maruo, T. Hasegawa, and N. Yoshimura, "Single-anchor support and supercritical CO2 drying enable high-precision microfabrication of three-dimensional structures," Optics Express, vol. 17, no. 23, pp. 20945-20951, 2009.
[29]C.-H. Hoi, "Design and Fabrication of Micro-Lens Arrays by Two-Photon Polymerization," M.S. thesis, National Taiwan University, Taiwan, 2015.
[30]W.-J. Lee, "Optimization of material and fabrication process for micro fabrication by Two-Photon Polymerization," M.S. thesis, National Taiwan University, Taiwan, 2014.
[31]K. Takada, H.-B. Sun, and S. Kawata, "Improved spatial resolution and surface roughness in photopolymerization-based laser nanowriting," Applied Physics Letters, vol. 86, no. 7, p. 071122, 2004.
[32]D. Wu, S.-Z. Wu, L.-G. Niu, Q.-D. Chen, R. Wang, J.-F. Song, H.-H. Fang, and H.-B. Sun, "High numerical aperture microlens arrays of close packing," Applied Physics Letters, vol. 97, no. 3, p. 031109, 2010.
[33]Y.-H. Hsu, S. Shivani, A. Liu, Q.-W. Tang, C.-J. Lee, A.-B. Wang, T.-T. Chung, "Migration Analysis of Osteosarcoma MG-63 Cells on Roughened Substrates Created by Two Photon polymerization," in The 2016 Tissue Engineering and Regenerative Medicine International Society- Asia Pacific Meeting, Taiwan, 2016.
[34]S. Shivani, A. Liu, C.-J. Lee, Q.-W. Tang, A.-B. Wang,T.-T. Chung, Y.-H. Hsu, "Guided migration analysis of osteosarcoma MG-63 cells on graded roughened substrates created by two photon polymerization," in 2016 IEEE EMBS Micro and Nanotechnology in Medicine Conference, Hawaii, 2016.
[35]S. Maruo, T. Hasegawa, and N. Yoshimura, “Single-anchor support and supercritical CO2 drying enable high-precision microfabrication of three-dimensional structures,” Optics Express, vol. 17, no. 23, p. 20945, Nov. 2009.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊