臺灣博碩士論文加值系統

Table of Contents
Recommendation Letter from the Thesis Advisor ...
Dissertation Oral Defense Committee Certification.
Acknowledgments iii
Chinese Abstract iv
English Abstract vii
Table of Contents x
List of Figures xii
List of Tables xiv
Chapter 1 Background and Introduction 1
Section 1.1 Adenosine deaminase that acting on RNAs (ADARs) family 1
Section 1.2 Long noncoding RNAs 3
Section 1.3 Interferon-inducible protein kinase RNA activator (PRKRA / PACT) 5
Section 1.4 Unc80, Nalcn channel complex subunit (c2orf21 / KIAA1843) 7
Chapter 2 Materials and Methods 9
Chapter 3 Results 22
Section 3.1 A novel antisense RNA ASPACT confers multi-level suppression of PACT and associated signaling 22
Section 3.2 The role of RNA editing in the regulation of ion channel complex scaffold protein, Unc80 37
Chapter 4 Discussion 42
References 51
Figures 62
Table 100



List of Figures
Figure 1. The editing sites of PACT were labeled in UCSC database..... 62
Figure 2. The A-to-I editing events were confirmed by Sanger sequencing
and RNA immuno-precipitation assay .... 63
Figure 3. The A-to-I editing events were confirmed by Sanger sequencing
and RNA immuno-precipitation assay .... 65
Figure 4. The expression of the ASPACT transcript unit ... 67
Figure 5. The nascent RNA of ASPACT is edited by ADAR1 . 70
Figure 6. RNA expression level of ASPACT is regulated by RNA editing.
... 71
Figure 7. Expression level of PACT is affected by ADAR1 ...... 73
Figure 8. Regulatory role of ASPACT in PACT expression 75
Figure 9. ASPACT negatively regulates PACT expression at the
transcriptional level ..... 77
Figure 10. ASPACT associates with HDAC1 and mediates its promoter
recruitment ..... 80
Figure 11. ASPACT mediates nuclear retention of PACT mRNA via
direct RNA–RNA interaction .... 82
Figure 12. Regulation at the post-transcriptional level has a greater impact
on the abundance of PACT protein .. 84
Figure 13. Functional link of ASPACT to the innate immunity 85
Figure 14. Functional link of ASPACT to the innate immunity 87
Figure 15. ASPACT overexpression does not affect the translational rate
of PACT mRNA ... 89
Figure 16. Schematic models of RNA editing in the regulation of
ASPACT and multi-level suppression of ASPACT on PACT ... 90
Figure 17. The confirmation of editing site on Unc80 . 92
Figure 18. The genes of Nalcn channel complex are not affected by
Unc80 overexpression . 93
Figure 19. The calcium influx was altered by RNA editing in N2a cells ....
... 94
Figure 20. The habituation and dishabituation test of Unc80 knock-in
mice .. 95
Figure 21. The RNA and protein expression levels in olfactory bulb of
Unc80 knock-in mice in response to isoamyl acetate treatment 96
Figure 22. The ladder walking test of Unc80 knock-in mice ..... 97
Figure 23. The rotarod performance test of Unc80 knock-in mice ... 98
Figure 24. The triple horizontal bars test of Unc80 knock-in mice .. 99

List of Tables
Table 1. Primer sequence .. 100

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