跳到主要內容

臺灣博碩士論文加值系統

(100.28.132.102) 您好!臺灣時間:2024/06/21 22:19
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:王可璇
研究生(外文):WANG, KO-HSUAN
論文名稱:香蕉花萃取物之生物活性評估
論文名稱(外文):The Bioactive Evaluation of the Extract Flowers of Musa paradisiaca
指導教授:黃和全
指導教授(外文):HUANG, HO-CHENG
口試委員:黃和全葉安祺鄭竣亦
口試委員(外文):HUANG, HO-CHENGYEH, AN-CHICHENG, SHI-YIE
口試日期:2019-06-24
學位類別:碩士
校院名稱:正修科技大學
系所名稱:化妝品與時尚彩妝研究所
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:中文
論文頁數:88
中文關鍵詞:香蕉花抗氧化多醣體
外文關鍵詞:banana flowerantioxidantpolysaccharide
相關次數:
  • 被引用被引用:0
  • 點閱點閱:302
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0

中文摘要
香蕉(Musa paradisiaca)是多年生單子葉巨型草本植物,於熱帶、亞熱帶地區作為高營養價值的植物,然而香蕉花會在香蕉生長過程中搶奪其養分,故被摘除丟棄成為農業廢棄物,但香蕉植物本身帶有的生物活性化合物如維生素C、E、酚類化合物、類胡蘿蔔素、生物胺、植物固醇、礦物質、膳食纖維等。
本研究利用不同溶劑萃取所得香蕉花萃取物之抗氧化成分、能力、抑制酪胺酸酶及保濕能力檢測和探討香蕉花乙醇萃取物經過濃縮、純化、分離的過程所得萃取物之抗氧化能力測試檢測後,依其結果比較萃取物在何種條件下於各項檢測為最佳。
實驗結果顯示萃取物之抗氧化能力,其DPPH自由基清除能力測定,清除率以乙醇萃取物(94.57 %)>乙酸乙酯萃取物(69.56 %)>水萃取物其清除力(61.64 %);經分離純化後香蕉花萃取物方面清除率以B3萃取物(96.54 %)>B1萃取物(13.83 %)>B2萃取物(9.62 %)。亞鐵離子螯合能力測定,以乙醇萃取物(95.25 %)>乙酸乙酯萃取物(78.05 %)>水萃取物(53.49 %);經分離純化後香蕉花萃取物方面螯合能力以B3萃取物(96.61 %)>B1萃取物(89.76 %)>B2萃取物(82.09 %)。還原力測定以乙醇萃取物(0.811)>乙酸乙

酯萃取物(0.455)>水萃取物(0.267);經分離純化後香蕉花萃取物方面還原力以B3萃取物(0.907)>B1萃取物(0.196)>B2萃取物(0.138)。
香蕉花萃取物抗氧化成分實驗,總酚含量以乙醇萃取物(89.6 mg GA/g d.w.)>水萃取物(16.8 mg GA/g d.w.)>乙酸乙酯萃取物(8.32 mg GA/g d.w.)。總類黃酮含量測定以乙醇萃取物(4.9 mg Rutin/g d.w.)>水萃取物(5.10 mg Rutin/g d.w.)>乙酸乙酯萃取物(3.68 mg Rutin/g d.w.)。多醣體含量測試以標準品葡萄糖進行總醣含量測定,水萃取物總醣含量(59.52 glucose/g d.w.);以標準品葡萄糖進行還原醣含量測定,水萃取物其還原醣含量(8.16 glucose/g d.w.)。體外抑制酪胺酸酶試驗,以乙醇萃取物的抑制率(79.79 %)>乙酸乙酯萃取物(51.61 %)>水萃取物(43.16 %)。吸水率測試以水萃取物(16.96 %)>乙酸乙酯萃取物(1.44 %)>乙醇萃取物(1.26 %)。保濕率測試以水萃取物(45.52 %)乙酸乙酯萃取物(27.78 %)>乙醇萃取物(26.98 %)。
Banana (Musa paradisiaca), a perennial monocotyledonous herb, is popular as a highly nutritious plant in tropical and subtropical regions. However, banana flower robs its nutrients during the growth of bananas and is therefore removed and discarded as agricultural waste. Banana plants themselves contain biologically active compounds such as vitamins C, E, phenolic compounds, carotenoids, biogenic amines, plant sterols, minerals, and dietary fiber.

In this study, the antioxidant components, ability, inhibition of tyrosinase and moisturizing ability of the extracted banana flower extracts were extracted by different solvents, and the antioxidant capacity of the extract obtained by the process of concentration, purification and separation of the ethanol extract of banana flower was investigated. After the test, the results of the extract were compared under the conditions of the test.

The experimental results showed that the antioxidant capacity of the extract was determined by DPPH free radical scavenging ability, and the clearance rate was ethanol extract (94.57%) > ethyl acetate extract (69.56 %) > water extract removal power (61.64%); The clearance of banana flower extract after separation and purification was B3 extract (96.54%) > B1 extract (13.83 %) > B2 extract (9.62%). The chelating ability of ferrous ions was determined by ethanol extract (95.25 %) > ethyl acetate extract (78.05 %) > water extract (53.49%); after extraction and purification, the chelation ability of banana flower extract was extracted by B3 (96.61%) > B1 extract (89.76 %) > B2 extract (82.09%). Reducing power was determined by ethanol extract (0.811) > ethyl acetate extract (0.455) > water extract (0.267); reduction and purification of banana flower extract after separation and purification with B3 extract (0.907) > B1 Extract (0.196) > B2 extract (0.138).

Antioxidant component of banana flower extract, total phenolic content as ethanol extract (89.6 mg GA/g d.w.) > water extract (16.8 mg GA/g d.w.) > ethyl acetate extract (8.32 mg GA) /g d.w.). The total flavonoid content was determined as ethanol extract (4.9 mg Rutin/g d.w.) > water extract (5.10 mg Rutin/g d.w.) > ethyl acetate extract (3.68 mg Rutin/g d.w.). The polysaccharide content test was carried out by measuring the total sugar content of the standard glucose, the total sugar content of the water extract (59.52 glucose/g d.w.); the determination of the reducing sugar content by the standard glucose, and the reducing sugar content of the water extract was (8.16 glucose/ g d.w.). Inhibition of the tyrosinase assay in vitro, inhibition rate of ethanol extract (79.79 %) > ethyl acetate extract (51.61%) > water extract (43.16%). The water absorption test was carried out with water extract (16.96 %) > ethyl acetate extract (1.44%) > ethanol extract (1.26%). The moisture retention test was carried out with water extract (45.52%) ethyl acetate extract (27.78%) > ethanol extract (26.98 %).
目錄
壹、 緒論 1
1-1、 前言 1
1-2、 研究背景與動機 2
1-3、 研究目的 2
1-4、 香蕉花的介紹 2
1-5、 活性氧自由基 (Reactive oxygen species radicals) 和抗氧化(Antioxidant) 10
貳、 文獻回顧 14
2-1、 香蕉花相關文獻 14
2-2、 化合物相關文獻 15
參、 實驗方法與材料 19
3-1、 實驗架構 22
3-2、 實驗方法 24
3-3、 抗氧化能力檢測 26
3-3-1、 DPPH自由基清除能力測定 26
3-3-2、 亞鐵離子螯合能力測定 28
3-3-3、 還原力測定 30
3-4、 抗氧化成分檢測 32
3-4-1、 總酚含量測定 32
3-4-2、 總類黃酮含量測定 34
3-5、 多醣體含量檢測 36
3-5-1、 總醣含量測定 36
3-5-2、 還原醣含量測定 38
3-6、 美白試驗 40
3-7、 保濕能力檢測 42
肆、 結果與討論 43
4-1、 抗氧化能力檢測 43
4-1-1、 DPPH自由基清除能力測定 43
4-1-2、 亞鐵離子螯合能力測定 47
4-1-3、 還原力測定 51
4-2、 抗氧化成分檢測 55
4-2-1、 總酚含量測定 55
4-2-2、 總類黃酮含量測定 57
4-3、 多醣體含量檢測 59
4-3-1、 總醣含量測定 59
4-3-2、 還原醣含量測定 60
4-3-3、 多醣體含量測 60
4-4、 體外抑制酪胺酸酶試驗 61
4-5、 保濕能力測定 63
伍、 結論與建議 66
陸、 參考文獻 69


1、中國歷代女子妝容,江蘇鳳凰文藝出版社,李芽,2017。
2、天然中草藥化妝品學,華格那出版社,邱怜瑋、丁秀玉、張淑微、賴雅韻、黃和全、汪慧娟、黃文盈,2016. 8月初版。
3、Sadia Qamar, Azizuddin Shaikh. Therapeutic Potentials and Compositional Changes of Valuable Compounds from Banana – A Review Trends in Food Science&Technology, 2018, 79, 1-9.
4、Lassoudière André. Le Bananier Et Sa Culture. Versailles: Ed. Quae, 2007. 383.
5、Jones D.R. Diseases of banana Abaca and Enset. In: Jones, D.R. (Ed.), CABI, United Kingdom, 1999, 1-36.
6、Stover R. H., Simmonds N. W. Classification of Banana Cultivars. In: Stover RH and Simmonds NW (ed.) Bananas, 3rd Edn. Wiley, New York, 1987, 5-9.
7、Stover R. H., Simmonds N. W. Classification of Banana Cultivars. In: Stover RH and Simmonds NW (ed.) Bananas, 3rd Edn. Wiley, New York, 1987, 13-17.
8、張智超(2008),利用高效液相層析法對香蕉花中β-sitosterol及stigmasterol的定量研究,國立台東大學生命科學研究所碩士論文。
9、Nimisha Sarah Mathew, Pradeep Singh Negia Traditional Uses, Phytochemistry and Pharmacology of Wild Banana (Musa acuminata Colla): A review, Journal of Ethnopharmacology, 2017, 196, 124-140.
10、J. Houghton, I.M. OsibogunReview Article - Flowering Plants Used Against SnakebiteJ. Ethnopharmacol., 1993,39, l-292.
11、Subbaraya Farmers Knowledge of Wild Musa. in India Food and Agriculture Organization of The United States, Rome, 2006.
12、H. De Wet, M. Ramulondi, Z.N. Ngcobo, The use of indigenous medicine for the treatment of hypertension by a rural community in northern Maputaland, South Africa, South Afr. J. Bot., 2016, 103, 78-88.
13、K. Majumdar, R. Saha, B.K. Datta, T. Bhakta Medicinal plants prescribed by different tribal and non-tribal medicine men of Tripura state, Ind. J. Trad. Knowl., 2006, 5, 559-562.
14、G. Rabbani, M.J. Albert, H. Rahman, A.K. Chowdhury Short-chain fatty acids inhibit fluid and electrolyte loss induced by cholera toxin in proximal colon of rabbit in vivo Digestive Diseases and Sciences, 1999,44, 1547-1553.
15、V. Chintamunnee, M.F. Mahomoodally Herbal medicine commonly used against non-communicable diseases in the tropical island of Mauritius, J. Herb. Med., 2012, 2, 113-125.
16、J.E. Okon, G.J. Esenowo, I.P. Afaha, N.S. Umoh Haematopoietic properties of ethanolic fruit extract of Musa acuminata on albino rats Bull. Environ. Pharmacol., Life Sci., 2013, 2, 22-26.
17、David, A. L., William, N. F., Graham, P. S., J.Ethnopharm. 1999, 65, 283-288.
18、Lewis, D. A., Fields, W. N., Shaw, G. P. A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. Journal of Ethnopharmacology, 1999, 65, 283–288.
19、Lewis, D. A., & Shaw, G. P. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions. The Journal of nutritional biochemistry, 2001, 12, 95–100.
20、Alisi C., Nwanyanwu C., Akujobi C., Ibegbulem C. Inhibition of dehydrogenase activity in pathogenic bacteria isolates by aqueous extracts of Musa paradisiaca (Var Sapientum). African Journal of Biotechnology, 2008, 7.
21、Mallick, C., Maiti, R., Ghosh, D. Comparative Study on Antihyperglycemic and Antihyperlipidemic Effects of Sparate and Composite Extract of Seed of Eugenia Jambolana and Root of Musa Paradisiaca in Streptozotocininduced Diabetic Male Albino Rate. Iranian Journal of Pharmacology and Therapeutics, 2006, 5, 27–33.
22、Pari, L., & Maheswari, J. U. Hypoglycaemic effect of Musa sapientum L. in alloxan-induced diabetic rats. Journal of Ethnopharmacology, 1999, 68, 321–325.
23、Ulrike Blume-Peytavi, David A. Whiting, Ralph M. Trüeb. Hair Growth and Disorders. Springer Science & Business Media. 26 June, 2008, 368–370.
24、Liu, R. H.: Health-promoting components of fruits and vegetables in the diet. Advances in Nutrition: An International Review Journal, 2013, 4, 384S–392S.
25、Hang T. Vu, Christopher J. Scarlett, Quan V. Vuong Phenolic compounds within banana peel and their potential uses: A review Journal of Functional Foods, 2018, 40, 238–248.
26、Crozier A., Yokota T., Jaganath I. B., Marks S. C., Salmarsh M., Clifford M. N. Secondary metabolites in fruits, vegetables, beverages and other plantbaseddietary components. In: A. Crozier, M. N. Clifford, & H. Ashihara (Eds.). Plant secondary metabolites: Occurrence, structure and role in the human diet Oxford (UK), Iowa (USA), Victoria (Australia): Blackwell Publishing Ltd. 2006.
27、Ostlund R. E., Racette S. B., Stenson W. F. Inhibition of cholesterol absorption by Phytosterol-replete wheat germ compared with Phytosterol-depleted wheat germ. Am. J. Clin. Nutr. 1 June, 2003, 77 (6).
28、張儷娟(2013) ,香蕉雄花的植物固醇含量及多酚類抗氧化能力測定,大仁科技大學食品科技系(所)碩士學位論文。
29、Knapp F. F., Nicholas H. J. The sterols and triterpenes of banana peel. Phytochemistry, 1969, 8, 207–214.
30、Balwinder Singh, Jatinder Pal Singh, Amritpal Kaur, Narpinder Singh Bioactive compounds in banana and their associated health benefits – A review, Food Chemistry , 2016, 206, 1–11
31、Udenfriend S., Lovenberg W., Sjoerdsma A. Physiologically active amines in common fruits and vegetables. Archives of Biochemistry and Biophysics, 1959, 85, 487–490
32、González-Montelongo R., Lobo M. G., González M. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds, Food Chemistry, 2010, 119, 1030–1039.
33、Vijayakumar S., Presannakumar G., Vijayalakshmi N. R. Antioxidant activity of banana flavonoids. Fitoterapia, 2008, 79, 279–282.
34、Bennett R. N., Shiga T. M., Hassimotto N. M., Rosa E. A., Lajolo F. M., Cordenunsi B. R.. Phenolics and antioxidant properties of fruit pulp and cell wall fractions of postharvest banana (Musa acuminata Juss.) cultivars. Journal of Agricultural and Food Chemistry, 2010, 58, 7991–8003.
35、Hayyan M., Hashim M.A., AlNashef I.M. "Superoxide Ion: Generation and Chemical Implications". Chem. Rev. 2016, 116 (5), 3029-3085
36、Loft S., Poulsen H. E. Cancer risk and oxidative DNA damage in man. Journal of Molecular Medicine, 1996, 74, 297-312.
37、Devasagayam T. P., Tilak J. C., Boloor K. K., Sane K. S., Ghaskadbi S. S., Lele R. D. "Free radicals and antioxidants in human health: current status and future prospects". The Journal of the Association of Physicians of India, October, 2004, 52, 794–804.
38、Kovacic P., Pozos R. S., Somanathan R., Shangari N., O'Brien P. J. Mechanism of mitochondrial uncouplers, inhibitors, and toxins: focus on electron transfer, free radicals, and structure-activity relationships. Curr Med Chem, 2005, 12, 2601-23.
39、Reiter R. J., Melchiorri D., Sewerynek E., Poeggeler B., Barlow-Walden L., Chuang J., Ortiz G.G., Acuña-Castroviejo D. A review of the evidence supporting melatonin's role as an antioxidant. J Pineal Res, Jan, 1995, 1,1-11.
40、Laloi C., Havaux M. "Key players of singlet oxygen-induced cell death in plants". Frontiers in Plant Science, 2015, 6, 39.
41、夏浩翔(2011),香蕉雄花抗癌活性成分研究。微生物免疫與生物藥學系研究所。國立嘉義大學。
42、楊海云(2011),香蕉皮多醣對人乳腺癌 MCF_7 細胞體外作用及其機制的研究,病理學與病理生理學,桂林醫學院。
43、蕭似帆(2013),香蕉不同萃取物對Th1/Th2免疫平衡、發炎及PC-3與MCF-7細胞凋亡之影響,食品暨應用生物科技學系所,國立中興大學。
44、曾國香(2015),香蕉花苞片及雄性花萃取物之抗氧化及肝細胞保護作用,食品科學系,國立屏東科技大學。
45、Dutta P. K., Dac A. K., Banerji N. Phytochem. 1983, 22(11), 2563–2564.
46、Akihisa T., Kimura Y., Kokke W. C. M. C., Takase S., Chem. Pharm. Bull. 1997, 45(4), 744–746.
47、Akihisa T., Kimura Y., Tamura T. Phytochem. 1998, 47, 1107–1110.
48、Shinichi Someya, Yumiko Yoshiki, Kazuyoshi Okubo Antioxidant compounds from bananas (Musa Cavendish). Food Chemistry, 2002, 79, 351–354.
49、Ramith Ramu, Prithvi S., Shirahatti, Farhan Zameer, Lakshmi V., Ranganatha M. N. Nagendra Prasad Inhibitory effect of banana (Musa sp. var. Nanjangud rasa bale) flower extract and its constituents Umbelliferone and Lupeol on α-glucosidase, aldose reductase and glycation at multiple stages. South African Journal of Botany, 2014, 95 54–63.
50、Yamaguchi T., Takamura H., Matoba T., Terao J., HPLC method for evaluation of the free radical-scavenging activity by foods by using 1,1-diphenyl-2-picrylhydrazyl. Biosci. Biotechnol. Biochem. 1988, 62(6), 1201-1204.
51、Dinis T. C. P., Madeira V. M. C., Almeida L. M. Action of Phenolic Derivatives as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers. Archives of Biochemistry and Biophysics, 1994, 315, 161-169.
52、Negev.http://www.bgu.ac.il/life/mizrahi/article1.html / Yen G. C., Chen H. Y. Antioxidant Activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem, 1995, 46, 849-854.
53、Oyaizu M.: Studies on products of browning reaction Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr, 1986, 44, 307.
54、Singleton V. L., Orthofer R., Lamuela-Raventos R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology,1999, 299, 152-178.
55、Christel Q. D., Bernard G., Jacques V., Thierry D., Claude B., Michel L., Micheline C., Jean-Cluade C., Francois B., Francis T. Phenolic compounds and antioxidant activities of buckwheat hulls and flour. J. Ethnopharmacology, 2000, 72, 35-42.
56、Dubois M., Gilles K. A., Hamilton J. K., et al. A colormetric method for the determination sugars, J. Nature, 1951, 28(7), 167-168.
57、齊香君、苟金霞、韓戌躇(2004),3,5-二硝基水楊酸比色法測定溶液中還原醣的研究,纖維素科學技術,3,17-20。
58、Martos M. V., Navajas Y. R., López J.F. Functional Properties of Honey,Propolis, and Royal Jelly. Journal of food scienice, 2008, 73(9), 117-124.
59、張麗卿,化妝品檢驗分析,華杏出版股份有限公司,2010年9月ㄧ版二刷。

電子全文 電子全文(網際網路公開日期:20240722)
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊