臺灣博碩士論文加值系統

　　鎂是人體中必要元素之一，鎂合金有良好的生物相容性和可降解性，有潛力成為骨科臨時性植入材料，為創傷或病變組織癒合期間提供暫時的機械支持。但鎂合金植入物在人體中有腐蝕過快的問題，因此，為了在人體中降低鎂合金腐蝕速率並探討鎂合金降解影響因素及生物相容性，且避免在植入部位修復前完全降解，表面處理技術被認為是鎂合金主要的改善方向。
　　本研究為探討AZ31B鎂合金在模擬人體血漿中降解速率等問題，運用微弧氧化處理(Micro-Arc Oxidation，MAO)改善耐腐蝕性，電解液採用矽酸鹽、氫氧化鈉及檸檬酸鈉配製，採用直流脈衝方式調整時間進行微弧氧化處理。實驗結果顯示AZ31B鎂合金經微弧氧化處理後(MAO-AZ31B)，於人工模擬體液(Simulated Body Fluid，SBF)透過腐蝕極化曲線分析，AZ31B微弧氧化30 min處理具有為最佳耐腐蝕性。將MAO-AZ31B浸泡在SBF中7天，從掃描式電子顯微鏡(Scanning Electron Microscope，SEM)、穿透式電子顯微鏡(Transmission electron microscope，TEM)及X射線光電子光譜(X-ray photoelectron spectroscopy，XPS)進行交叉比對，顯示SBF會從表面孔洞及裂縫進入微弧氧化膜層中降解，使多孔層之孔洞被磷灰石Apatite填滿，並有一層磷灰石沉積物覆蓋於MAO-AZ31B，根據原子濃度縱深分析，MAO-AZ31B浸泡SBF溶液中1天與7天相比Ca2p及P2p無顯著差異，認為浸泡1天已有一定厚度之磷灰石Apatite沉積層。以不同數目之AZ31B試片測試於細胞培養基48小時，對Neuro2a小鼠神經瘤細胞之存活情況，顯示會明顯抑制細胞生長並造成死亡。

　　Magnesium is one of the essential elements in the human body. Magnesium alloy has good biocompatibility and degradability, and has the potential to become a temporary orthopedic implant material, providing temporary mechanical support for wound or diseased tissue healing. However, magnesium alloy implants have the problem of excessive corrosion in the human body. Therefore, in order to reduce the corrosion rate of magnesium alloy in the human body and explore the factors and biocompatibility of magnesium alloy degradation, and avoid complete degradation before the repair of the implant site. Surface treatment technology is considered to be the main improvement direction of magnesium alloys.
　　In order to improve the degradation rate of AZ31B magnesium alloy in human plasma, this study used Micro-Arc Oxidation (MAO) to improve corrosion resistance. The electrolyte was prepared by using citrate, sodium hydroxide and sodium citrate. The micro-arc oxidation treatment is performed by adjusting the time by a direct current pulse method. The experimental results show that AZ31B magnesium alloy is treated by micro-arc oxidation (MAO-AZ31B), analyzed by corrosion polarization curve of Simulated Body Fluid (SBF), and AZ31B micro-arc oxidation for 30 min has the best corrosion resistance. MAO-AZ31B was immersed in SBF for 7 days, from Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and X-ray photoelectron spectroscopy (XPS). Cross-alignment shows that SBF will degrade from the surface pores and cracks into the micro-arc oxidation layer, so that the pores of the porous layer are filled with Apatite, and a layer of Apatite deposit covers the MAO-AZ31B, according to In the depth analysis of atomic concentration, there was no significant difference in Ca2p and P2p between MAO-AZ31B and SBF solution for 1 day and 7 days. It was considered that there was a certain thickness of Apatite deposit for 1 day. Survival of Neuro2a mouse neuroblastoma cells with different numbers of AZ31B test strips for 48 hours showed significant inhibition of cell growth and death.

目錄

封面內頁
簽名頁
中文摘要 ..iii
ABSTRACT...v
誌謝...vi
目錄...viii
圖目錄...xi
表目錄...xiii

第一章 前言...1
1.1 研究動機...1
1.2 研究目的...2

第二章 文獻回顧...4
2.1 鎂及其合...4
2.2 醫用金屬...6
2.3 微弧氧化處理...8

第三章 實驗流程...12
3.1 研究步驟...12
3.2 試片前處理及微弧氧化處理...13
3.3 體外生物活性測試...15
3.4 細胞測試...17
3.4 儀器設備...18
3.5 電化學量測與材料分析...20
3.5.1 電化學分析...20
3.5.2 分光儀分析...20
3.5.3 微硬度分析...20
3.5.4 XRD成分分析...21
3.5.5 FE-SEM及EDS分析...21
3.5.6 TEM分析...21
3.5.7 XPS分析...22
3.5.8 OM影像分析...22

第四章 結果與討論...23
4.1 研究結果...23
4.1.1 MAO-AZ31B微結構分析...23
4.1.2 MAO-AZ31B之腐蝕極化曲線分析...30
4.1.3 MAO-AZ31B於人工模擬體液(SBF)...31
4.2 結果與討論...35
4.2.1 MAO-AZ31B微結構與組成探討...35
4.2.2 鎂合金微弧氧化處理後於人工模擬體液(SBF)之TEM分析...39
4.2.3 微弧氧化處理後於人工模擬體液(SBF)之XPS成分分析...42
4.2.4 Ca/P比率...45
4.2.5 微弧氧化處理後人工模擬體液(SBF)之膜層降解反應機制...46
4.3 細胞試驗...47

第五章 結論...49
參考文獻...50

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