臺灣博碩士論文加值系統

目 錄
誌　　謝 i
摘　　要 ii
Abstracts iv
第一章 緒論 1
第二章 文獻回顧 5
2-1 牙周病 5
2-2 介孔生醫活性玻璃(Mesoporous Bioactive Glass, MBG) 5
2-2-1介孔生醫活性玻璃之簡介 5
2-2-2介孔生醫活性玻璃之優勢 7
2-2-3介孔生醫活性玻璃之問題與改善方式 8
2-3 球型介孔生醫活性玻璃 8
2-3-1 熔融-焠火法(Melt-quenching methods) 9
2-3-2 溶膠-凝膠法(Sol-gel methods) 10
2-3-2-1鹼催化法(Base-catalyzed method) 12
2-3-2-2酸/鹼共催化法(Acid/base co-catalyzed method) 12
2-3-2-3微乳液輔助法(Microemulsion-assisted method) 12
2-4介孔生醫活性玻璃之藥物包埋 16
2-4-1介孔生醫活性玻璃吸附藥物方式 16
2-4-2介孔生醫活性玻璃之藥物釋放行為 17
2-5介孔生醫活性玻璃奈米球的改質 17
2-5-1金屬離子之功能 18
2-5-2 銀離子之抑菌性與抑菌機制 19
2-6 阿魏酸(Ferulic acid, FA) 21
2-7 研究目的 22
第三章 實驗材料與方法 23
3-1實驗材料與儀器 23
3-2實驗總流程圖 27
3-3前驅實驗流程 28
3-3-1球型介孔生醫玻璃的製備流程與實驗組別代號 28
3-3-2摻雜銀之球型MBG的製備流程與實驗組別代號 30
3-4後續實驗流程 32
3-4-1 實驗組別代號 32
3-4-2 球型MBG含浸阿魏酸流程 35
3-5材料物化性質分析 37
3-5-1氮氣吸/脫附等溫曲線分析(BET) 37
3-5-2穿透式電子顯微鏡(TEM) 39
3-5-3 傅立葉紅外線光譜儀(FTIR) 39
3-5-4 X-ray繞射分析(XRD) 40
3-5-5 紫外線/可見光分光光譜儀(UV-Vis spectrometers) 40
3-6抑菌測試 41
3-6-1抑菌定性測試 41
3-6-2抑菌定量測試 41
3-7體外(in vitro)生物相容性測試 42
3-7-1細胞培養 42
3-7-2萃取液之準備 43
3-7-3細胞毒性測試(Cytotoxicity test) 43
3-7-4細胞長期增生測試 44
3-7-5鹼性磷酸酶(Alkaline phosphatase, ALP)半定量與染色 44
3-8統計分析 45
第四章 前驅實驗 46
4-1篩選製備方法 46
4-1-1 微相結構分析 46
4-1-2 比表面積、孔體積及孔徑大小分析 47
4-2篩選鹼催化劑濃度 50
4-2-1微相結構分析 50
4-3篩選MBG的銀含量 54
4-3-1銀含量3、6、9 mol%之MBG抑菌分析 54
4-3-2不同銀含量之MBG細胞毒性分析 57
第五章 後續實驗 59
5-1不同銀含量MBG微相結構分析 59
5-2不同銀含量MBG比表面積、孔體積及孔徑大小分析 61
5-3不同銀含量MBG晶相分析 64
5-4各組別之官能基分析 65
5-5阿魏酸釋放曲線分析 67
5-6各組別之細胞毒性測試 69
5-7各組別之抑菌測試 76
5-8複合CPC之抑菌測試 79
5-9複合CPC之機械性質測試 81
5-10複合CPC之細胞毒性測試 82
5-11複合CPC之細胞增生測試 84
5-12複合CPC之鹼性磷酸酶(ALP)半定量測試 86
5-13複合CPC之鹼性磷酸酶(ALP)染色分析 88
第六章 結論 90
參考文獻 92


圖目錄
圖 1、介孔生醫活性玻璃形成原理示意圖 6
圖2、水包油型微乳液製備球型MBG之形成過程 (參考文獻繪製Liang et al, 2015) 13
圖3、雙相型微乳液製備球型MBG之形成過程(Shen et al, 2014) 15
圖4、介孔生醫活性玻璃藥物釋放機制示意圖(參考文獻繪製Xia & Chang, 2006) 16
圖5、銀離子抑菌機制示意圖(參考文獻繪製Marambio-Jones & Hoek, 2010) 21
圖6、阿魏酸化學結構式 22
圖7、製備含有銀粒子之球型MBG實驗流程圖 27
圖8、以鹼催化法與微乳液法製備球型MBG之實驗流程圖 28
圖9、微乳液法製備球型MBG含不同濃度Ag之實驗流程圖 30
圖10、製備含有銀粒子之球型MBG實驗流程圖 33
圖11、球型MBG含浸藥物之實驗流程圖 35
圖12、球型MBG含浸藥物之實驗流程圖 36
圖13、(a)氮氣吸/脫附之等溫吸附曲線類型；(b)遲滯曲線類型(Thommes et al., 2015) 38
圖14、各組別的微相結構分析(40kX) 46
圖15、各組別的氮氣吸/脫附曲線圖及(左上)孔徑大小分布圖 49
圖16、1M氨水製備球型MBG含銀0、3、6、9 mol%之TEM及EDS分析圖 51
圖17、3M氨水製備球型MBG含銀0、3、6、9 mol%之TEM及EDS分析圖 52
圖18、5M氨水製備球型MBG含銀0、3、6、9 mol%之TEM及EDS分析圖 53
圖19、各組別對大腸桿菌及金黃色葡萄球菌之抑菌測試(單位: mm) 54
圖20、各組別於大腸桿菌菌液中培養1、4、8、24、48小時之定量抑菌測試 56
圖21、各組別於金黃色葡萄球菌菌液中培養1、4、8、24、48小時之定量抑菌測試 56
圖22、各組別對L929細胞進行培養24小時之細胞毒性測試 57
圖23、各組別對L929細胞進行培養24小時之細胞型態觀察 58
圖24、0Ag、0.1 Ag、0.5 Ag、1 Ag之TEM及EDS分析圖 60
圖25、各組別的(a)氮氣吸/脫附曲線圖及(b)孔徑大小分布圖 63
圖26、各組別的XRD分析 64
圖27、各組別的官能基分析 66
圖28、含浸藥物後各組別之藥物累計釋放量曲線(n=10) 68
圖29、各組別對L929細胞進行培養24小時之細胞毒性測試 70
圖30、各組別對L929細胞進行培養24小時之細胞型態觀察 70
圖31、稀釋0.1Ag、0.5Ag、1Ag之萃取液後對L929細胞進行培養24小時之細胞毒性測試 72
圖32、稀釋0.1Ag、0.5Ag、1Ag之萃取液後對L929細胞進行培養24小時之細胞型態觀察 73
圖33、含浸藥物後各組別對L929細胞進行培養24小時之細胞毒性測試 75
圖34、含浸藥物後各組別對L929細胞進行培養24小時之細胞型態觀察 75
圖35、(a)含浸藥物前(b)含浸藥物後對大腸桿菌及金黃色葡萄球菌之抑菌定性測試(單位: mm) 77
圖36、含浸藥物前後對大腸桿菌菌液中培養1、4、8、24、48小時之抑菌定量測試 78
圖37、含浸藥物前後對金黃色葡萄球菌菌液中培養1、4、8、24、48小時之抑菌定量測試 78
圖38、含銀之球型MBG對(a)大腸桿菌與(b)金黃色葡萄球菌之抑菌定性測試(單位: mm) 80
圖39、CPC與C-1Ag不同wt.%之抗壓測試結果(n=3) 81
圖40、CPC、C-0Ag、C-0.5、C-1Ag對L929細胞進行培養24小時之細胞毒性定量測試結果 83
圖41、CPC、C-0Ag、C-0.5、C-1Ag對L929細胞進行培養24小時之細胞型態觀察 83
圖42、CPC、C-0Ag、C-0AgF、C-1Ag、C-1AgF與D1 cell做接觸性培養，培養不同時間之細胞活性分析(n=3) 85
圖43、CPC、C-0Ag、C-0AgF、C-1Ag、C-1AgF與D1 cell做接觸性培養，培養不同時間之吸光值(n=3) 87
圖44、CPC、C-0Ag、C-0AgF、C-1Ag、C-1AgF與D1 cell做接觸性培養，培養不同時間之ALP半定量分析(n=3) 87
圖45、CPC、C-0Ag、C-0AgF、C-1Ag、C-1AgF與D1 cell做接觸性培養，培養不同時間之ALP染色定性分析 89
 
表目錄
表1、MBG之臨床應用(Arcos & Vallet-Regí, 2010) 7
表2、實驗材料 23
表3、實驗儀器 25
表4、球型MBG的不同製備方式與條件代號 29
表5、球型MBG的催化劑濃度與銀含量代號 31
表6、不同銀含量及藥物代號 32
表7、複合CPC後之代號 32
表8、各組別的比表面積、孔體積及孔徑大小與粒徑分析(n=20) 48
表9、不同催化劑濃度與銀含量之球型MBG粒徑分析(n=20) 50
表10、各組別的比表面積、孔體積及孔徑大小與粒徑分析(n=20) 62
表11、樣本萃取液稀釋倍率與相對應之萃取液粉液比 72

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