果皮色差與後熟果皮轉色相關，可溶性固形物含量之變化受到呼吸作用影響，可滴定酸變化會隨著後熟階段而升高，本試驗經不同殺菌處理中發現20 KV．cm-2處理後提升可溶性固形物之含量及果實色差，果實硬度之變化會受到果膠降解而改變，果膠降解程度與果實成熟度相關，本試驗發現施用溫湯處理後果實硬度下降較緩慢，經溫湯處理及高壓靜電場20 KV．cm-2處理能夠有效減低芒果病斑指數，且不影響果實酚類化合物及類黃酮化合物之含量， POD活性之改變除了因植物受到病原菌攻擊外其在衰老後期POD活性也會急劇上升，施用溫湯處理和20 KV．cm-2處理可延緩POD活性上升；在不同抑菌處理中施用果蠟可以提升果實色差外，對於可溶性固形物、可滴定酸及果實硬度則是沒有顯著的效果，對於病斑指數也沒有顯著效果，而施用幾丁聚醣處理可延緩POD活性上升，但抑菌處理對於炭疽病的發生是沒有影響。

The peel color is related ripened of mango. The content of total soluble solids is affected by respiration. The titratable acid will increase with the post-ripe stage.In different fungicidal treatments found 20 KV．cm-2 treatment the content of total soluble solids and the peel color of the mango are increased.The firmness will be changed by the degradation of pectin. The degree of pectin degradation is related to the post-ripe of the mango.The hot water treatment and high voltage electrostatic field 20 KV．cm-2 treatment can effectively reduce disease severity index, and does not affect the content of phenolic compounds and flavonoids in the mango.The POD activity increased caused by attacked by pathogenic bacteria and the POD activity to increase sharply in the later stages of aging. The 20 KV．cm-2 treatment can delay the increase of POD activity.In different antifungal treatment it can improve the peel color of mango,but has no significant effect on toatl soluble solids, titratable acids, frimness and disease severity index. The application of chitosan treatment can delay the increase of POD activity.But the different antifungal treatment has no effect on the occurrence of anthracnose.

目錄
表目錄 I
圖目錄 III
中文摘要 V
英文摘要 VI
壹、 前言（Introduction） 1
貳、前人研究（Literature Review） 3
一、芒果概說 3
二、炭疽病 4
三、病原菌與植物體內自由基清除之方式 5
四、高壓靜電場概論 6
五、電解水概論 8
六、溫湯處理概論 11
七、幾丁聚醣概論 13
八、果蠟概論 15
參、材料與方法（Materials and Methods） 18
試驗一、不同殺菌處理對炭疽病之影響 18
試驗二、不同抑菌處理對炭疽病之影響 19
肆、結果（Results） 24
伍、討論 (discussion) 61
陸、結論 (Conclusion) 80
柒、參考文獻（References） 82

1.丁樑泉. 2011. 果膠與果膠酵素國際研討會. 科學發展月刊29(10)：760-762.
2.中華民國行政院農委會農業統計年報. 2018.
3.丹陽、李里特. 2005. 高壓靜電場對番茄採後成熟、衰老過程的調控及其機理研究. 中國農業大學博士論文. 北京.
4.王奕程、方煒. 2006. 高壓靜電場在生物產業的應用. 臺灣大學生物產業機電工程研究所. 台北.
5.王愈、王寶剛、李里特. 2009. 靜電場處理對貯藏番茄品質及生理變化的影響. 農業工程學報 25(7)：288-293.
6.吳志鴻. 2006. 林產研究新思維-抗氧化活性物質之利用潛能. 林業研究專訊. 13(4)：1-4
7.段中漢、張智凱、王群中. 2017. 芒果炭疽病之藥劑防治與熱處理. 臺灣農藥學.3：65-78.
8.洪克前、弓德強、張魯斌、谷會、王松標、詹儒林. 2012. 殼聚醣塗膜對芒果採後品質及生理特性的影響. 中國農業通報. 28(28)：182-187.
9.徐旭方、柳葉飛、劉詩揚、單秀峰、王昇厚. 2016. 1-MCP結合殼聚醣處理延緩芒果果實後熟與衰老的研究. 食品工業. 27(32)：92-94
10.張嵐雁、石佩玉、張錦興. 2018. 愛文芒果溫湯處理模擬試驗. 臺南區農業改良場研究彙報57-69
11.郭紅蓮、程根武、陳捷、馮晶、崔澍、唐樹戈. 2003. 玉米灰班病抗性反應中酚類物質代謝作用的研究. 植物病理學報. 33(4)：342-346.
12.蔣永正. 2011. 植物對環境逆境之調控與運用. 農政與農情 231：78-85.
13.陳柏文. 2015. 耀上國際舞台的臺灣精品-芒果. 農政與農情 278：11-15.
14.陳慶、曾一凡. 2015. 高壓靜電場對採後綠蘆筍生理特性影響的研究. 江西農業大學碩士論文. 南昌.
15.曾敏南、陳昱初. 2014. 炭疽病菌侵染過程的重要基因. 高雄區農業改良場. 24(2)：25-38.
16.黃偉峻. 2013. 熱處理在園藝產品採後處理之應用.〈https://kmweb.coa.gov.tw/category/categorycontent.aspx?ReportId=286598&CategoryId=2&ActorType=002&kpi=0〉
17.葉青、李里特. 2004. 高壓靜電場保鮮裝置改進及對幾種呼吸跌變型果實的影響. 中國農業大學碩士論文. 北京.
18.趙麗芹、張子德. 2009. 園藝產品貯藏加工學(第二版). 中國輕工業出版社.p.29-32.
19.劉淑宇、陳發河、于新. 2013. 綠色木黴菌發酵液對芒果炭疽菌抑制作用及採後保鮮效果研究. 集美大學碩士論文. 廈門.
20.謝慶昌、薛淑滿. 2005. 芒果外銷之菜後處理及流程. 園產品採後處理技術之研究及應用研討會專刊.p. 14-20.
21.Al-haq, M.I., Y. Seo, S. Oshita and Y. Kawagoe. 2002. Disinfection effects of electrolyzed oxidizing water on suppressing fruit rot of pear caused by Botryosphaeria berengeriana.Food Research International 35：657–664.
22.Al-Qurashi A. D. and M. A. Awad. 2015. Postharvest chitosan treatment affects quality, antioxidant capacity,antioxidant compounds and enzymes activities of ‘El-Bayadi’ tablegrapes after storage. Scientia Horticulturae 197：392-398
23.Alvindia, D.G. and M.A. Acda. 2015. Revisiting the efficacy of hot water treatment in managing anthracnose and stem-end rot diseases of mango cv. ‘Carabao’. Crop Protection 67：96-101.
24.Amulya, P.R., K.P. Sudheer, E.J. Rifna, K.R. Sreesha and C. Nusaiba. 2016. Effect of edible wax coating and map on the quality of mango during storage. International Journal of Agricultural Science and Research 6：13-18.
25.Anwar R. and A.U. Malik. 2007. Hot water treatment affects ripening quality and storage life of mango (Mangifera indica L.). Pak. J. Agri. Sci. 44(2)：304-311.
26.Arauz, L.F. 2000. Mango anthracnose：economic impct and current optins for integrated management. Plant Diease 84：601-611.
27.Baloch, M.K. and F. Bibi. 2012. Effect of harvesting and storage conditions on the post harvest quality and shelf life of mango (Mangifera indica L.) fruit. South African Journal of Botany 83：109-116.
28.Bessi H., H. Debbabi, K. Grissa and S. Bellagha. 2014. Microbial reduction and quality of stored date fruits treated by electrolyzed water. Journal of Food Quality 37：42–49.
29.Chen Y., Y.C. Hung, M. Chen, M. Lin and H. Lin. 2019. Enhanced storability of blueberries by acidic electrolyzed oxidizing water application may be mediated by regulating ROS metabolism. Food Chemistry 270：229–235.
30.Chien, P.J., F. Sheu and F.H. Yang. 2017. Effects of edible chitosan coating on quality and shelf life of sliced mango fruit. Journal of Food Engineering 78：225–229.
31.Dalvi-Isfanhan, M.,N. Hamdami, A. Le-Bail and E. Xhanthakis. 2016. The principles of high voltage electric field and its application in food processing: A review. Food Research International. p. 1-47.
32.Dea S., J.K. Brecht, M.C.N. Nunes amd E.A. Baldwin. 2010. Quality of fresh-cut ‘Kent’ mango slices prepared from hot water or non-hot water-treated fruit. Postharvest Biology and Technology 56 ：171–180.
33.Dhall, R.K. 2013. Advances in Edible Coatings for Fresh Fruits and Vegetables: A Review. Critical Reviews in Food Science and Nutrition.53：435-450.
34.Eshetu A., A.M. Ibrahim, S.F. Forsido and C.G. Kuyu. 2018. Effect of beeswax and chitosan treatments on quality and shelf life of selected mango (Mangifera indica L.) cultivars. Heliyon 4：1-22.
35.Guentzal, J.L., K.L. Lam, M.A. Callan, S.A. Emmons and V.L. Dunham. 2008. Reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. Food Microbiology 25： 36–41.
36.Hu H.,H. Zhou and P. Li. 2019. Lacquer wax coating improves the sensory and quality attributes of kiwifruit during ambient storage. Scientia Horticulturae 244：31-41.
37.Huang, Y.R., Y.C. Hung, S.Y. Hsu, Y.W. Huang and D.F. Hwang. 2008. Application of electrolyzed water in the food industry. Food Control 19：329–345.
38.Jongsri P., P. Rojsitthisak, T. Wangsomboondee and K. Seraypheap. 2017. Influence of chitosan coating combined with spermidine on anthracnose disease and qualities of ‘Nam Dok Mai’ mango after harvest. Scientia Horticulturae 224：180–187.
39.Kefialew Y. and A. Ayalew. 2008. Postharvest biological control of anthracnose (Colletotrichum gloeosporioides) on mango (Mangifera indica). Postharvest Biology and Technology 50：8-11.
40.Kim Y., A. J. Lounds-Singleton and S. T. Talcott. 2009. Antioxidant phytochemical and quality changes associated with hot water immersion treatment of mangoes (Mangifera indica L.). Food Chemistry 115：989–993.
41.Koseki S., K. Yoshida, S. Isobe and K. Itoh. 2004. Efficacy of Acidic Electrolyzed Water for Microbial Decontamination of Cucumbers and Strawberries. Journal of Food Protection 67：1247-1251.
42.Li X., X. Zhu, H. Wang, X. Lin, H. Lin and Chen. 2018. Postharvest application of wax controls pineapple fruit ripening and improves fruit quality. Postharvest Biology and Technology 136：99–110.
43.Liu, C.E.,W.J. Chen, P.H. Li, P.L. Lu and C.W. Hsieh. 2017. Effect of a high voltage electrostatic field (HVEF) on the shelf life of persimmons (Diospyros kaki). Food Science and Technology 75：236-242.
44.Matern, U. and R.E.,Kneusel. 1988. Phenolic compounds in plant disease resistance. Phytoparasitica 16：153–170.
45.Da Silva Andrade, L.B., Da Silva Julião, M.S., Carneiro Vera Cruz, R., Soares Rodrigues, T.H., Dos Santos Fontenelle, R.O., and Da Silva, A.L.C., 2018. Antioxidant and antifungal activity of carnauba wax powder extracts. Ind. Crop. Prod. 125, 220–227.
46.Nelson, S.C. 2008. Mango anthracnose (Colletotrichum gloeosporiodes). Plant Diease 48：1-9.
47.New Guyana Marketing Corporation. 2004. Waxing fruits and vegetables.Postharvest Handling Technical Bulletin No.33.p.2-10.
48.Pagno, C.H., A. Castagna,A. Trivellini,A. Mensuali-Sodi,A. Ranieri,E. A. Ferreira,A. D. O. Rios and S. H. Flores. 2017.The nutraceutical quality of tomato fruit during domestic storage is affected by chitosan coating. J Food Process Preserv.42：1-9.
49.Palanimuthu V., P. Rajkumar, V. Orsat, Y. Gariépy and G.S.V. Raghavan. 2009. Improving cranberry shelf-life using high voltage electric field treatment. Journal of Food Engineering 90：365–371.
50.Park, H.J. 1999. Development of advanced edible coatings for fruits. Trends in Food Science & Technology 10：254-260.
51.Pongener A., S. Sharma and S.K. Purbey. 2011. Heat Treatment of Fruits and Vegetables. Postharvest Disinfection of Fruits and Vegetables：p179-196.
52.Prakash O. and B.K.Pandey. 2000. Control of mango anthracnose by hot water and fungicides treatment. Indian Phytopath 53(1)：92-94.
53.Raghav, P. K., N. Agarwal and M. Saini. 2016. Edible coating of fruits and vegetables : A REVIEW. International Journal of Scientific Research and Modern Education 1：188-204.
54.Rinaudo M. 2006. Chitin and chitosan: Properties and applications. Prog. Polym. Sci. 31：603–632.
55.Seraypheap, K., S. Yimyong, T.U. Datsenka and A.K. Handa. 2011. Hot Water Treatment Delays Ripening-associated Metabolic Shift in ‘Okrong’ Mango Fruit during Storage. J. Amer. Soc. Hort. Sci. 136(6):441–451.
56.Shivashankara, K.S, S. Isobe, M.I. Al-Haq, M. Takenaka and T. Shina. 2004. Fruit Antioxidant Activity, Ascorbic Acid, Total Phenol, Quercetin, and Carotene of Irwin Mango Fruits Stored at Low Temperature after High Electric Field Pretreatment. J. Agric. Food Chem. 52：1281-1286.
57.Sripong K., P. Jitareerat, S. Tsuyumu, A. Uthairatanakij, V. Srilaong, C. Wongs-Aree, G. Ma, L. Zhang and M. Kato. 2015. Combined treatment with hot water and UV-C elicits disease resistance against anthracnose and improves the quality of harvested Mangoes. Crop Protection 77：1-8
58.Al-haq M. I.,J. Sugiyama and S. Isobe. 2005. Applications of Electrolyzed Water in Agriculture & Food Industries. Food Sci. Technol. Res. 11(2)：135-150.
59.Sui Y., S. Droby, D.F. Zhang, W.J. Wang and Y.S. Liu. 2014. Reduction of Fusarium rot and maintenance of fruit quality in melon using eco-friendly hot water treatment. Environ Sci Pollut Res 21:13956–13963.
60.Thinh, D.C. and K. Kunasakdakul. 2013. Inhibition of Colletotrichum gloeosporioides and control of postharvest anthracnose disease on mango fruit using propionic acid combined with Bee-Carnauba wax emulsion. J. Agr. Sci. 5(12)：110-116
61.Xing K., X. Zhu, X. Peng and S. Qin. 2014. Chitosan antimicrobial and eliciting properties for pest control in agriculture: a review. Agron. Sustain. Dev.
62.Zaho R., J. Hao, H. Liu and L. Li. 2011. Effect of high-voltage electrostatic field pretreatment on the antioxidant system in stored green mature tomatoes. J Sci Food Agric 91: 1680–1686.
63.Zhu X., Q. Wang, J. Cao and W. Jiang. 2008. EFFECTS OF CHITOSAN COATING ON POSTHARVEST QUALITY OF MANGO (MANGIFERA INDICA L. CV. TAINONG) FRUITS. Journal of Food Processing and Preservation 32：770–784.
64.Zong Y., J. Liu, B. Li, G. Qiu and S. Tian. 2010. Effects of yeast antagonists in combination with hot water treatment on postharvest diseases of tomato fruit. Biological Control 54：316–321.