臺灣博碩士論文加值系統

Chinese Abstract ii
English Abstract iii
Acknowledgements iv
Table of Contents v
List of Tables vii
List of Figures viii
Abbreviations ix
1 Introduction 1
1.1 The Importance of Bacterial Pathogen Detection 1
1.2 Traditional Methods of Bacteria Detection 1
1.3 Biosensors 2
1.4 Poly-Silicon Nanowire Field Effect Transistors 2
1.5 The Use of pSNWFETs as Whole Bacteria Sensors 4
1.6 Bacteria Samples 4
2 Material and Methods 5
2.1 Materials 5
2.2 Microfluidic System 5
2.3 Open-well System 6
2.4 Sensing System 6
2.5 pSNWFET Surface Cleansing with EKC 6
2.6 Electrical Characterization of pSNWFETs 7
2.7 Surface Modification of pSNWFETs 7
2.7.1 Oxygen Plasma Treatment 7
2.7.2 APTES modification 7
2.7.3 Glutaraldehyde modification 8
2.7.4 Antibody Immobilization 8
2.7.5 Blocking by NaBH3CN 8
2.8 Sample Preparation of Bacteria Targets 8
2.9 Testing Procedure with Microfluidic Channel System 8
2.10 Testing Procedure with Open-Well System 9
2.11 Verification of Surface Modification 10
2.11.1 Verification by SEM 10
2.11.2 Step-by-Step Id-Vg Curve Test 10
3 Results 12
3.1 The Electric Characteristics of the pSNWFET Chip 12
3.2 Lysed E. coli Sensing 12
3.3 Whole E. coli Cell Sensing 13
3.4 Whole Staphylococcus aureus Cell Sensing 13
3.5 Verification of Surface Modification by SEM 14
3.6 Step-by-Step Id-Vg Curve Test 15
4 Discussion 15
4.1 Comparison Between Microfluidic Channel System and the Open-Well System 15
4.2 The Electric Characteristics of the pSNWFET Chip 5
4.3 Comparison Between Lysed E. coli and Sensing Whole E. coli Cell Sensing 16
4.4 Whole Staphylococcus aureus Cell Sensing 16
4.5 Verification by SEM and Step-by-Step Id-Vg Curve Test 17
5 Conclusion 18
6 Reference 19
 

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