臺灣博碩士論文加值系統

為了解光刺激對於帕金森氏症大鼠之作用效果及有效作用的時效性，本研究論文參考過去光刺激大鼠大腦黑質緻密部的實驗所得出的最佳光刺激參數，以光波長840 nm的近紅外雷射光，光功率1 mW，持續照射五分鐘，對大鼠的黑質緻密部進行大腦深層光刺激及二次刺激實驗，並以旋轉木滾輪測試（Rota-rod Test）進行大鼠的行為量化分析，搭配腦波量測交互比對驗證神經系統與行為上變化的關聯性，希望找出光刺激效果及持續時間，尋找一套最佳的刺激週期。結果發現光刺激效果持續時間約為兩小時至三小時，而進行二次刺激仍然有明顯效果；且接受刺激後的帕金森氏症大鼠在滾輪上的時間明顯增加；另外在腦波分析得到刺激後腦波強度會有下降的趨勢，再比對先前化學分析的研究結果，谷氨酸濃度下降，多巴胺濃度上升的變化趨勢，與腦波及行為的變化趨勢上的彼此相關，三者之間是交互影響的。希望本研究結果能夠提供腦部深層光刺激治療相關應用有一個新的方向。

The aim of the project is to evaluate the effectiveness of light on rat with Parkinson disease. The project conducts an experiment to stimulate the SNc of Parkinson’s rat by the optimized optical parameters for previous research where an 840 nm, 1 mW light stimulation for an irradiating period of 5 minutes are provided. There will be a 2 or 3 hours break between the first stimulation and the second stimulation and a same interval before the final data collection. The rota-rod test and EEG results are analyzed by implementing quantitative analysis so that we can understand the effect of the behavior and neurotransmitter changes, respectively, on stimulated rats. In conclusion, the light stimulation can be kept active for 2 to 3 hours, and the time of rat on the rod increases with the stimulation duration. In addition, the trend of brainwave intensity decreases after the stimulation. For the second stimulation, a similar stimulus response corresponding to the first stimulation is also acquired, suggesting that there will be a decrease on brainwave intensity after the stimulation. Comparing the results of the brainwave and the rota-rod test with the previous chemical analysis, the experiment suggests that there is a certain connection between the descent of glutamate and the ascent of dopamine.

中文摘要 i
Abstract ii
致謝 iii
表目錄 v
圖目錄 vi
第一章 緒論 1
1.1 研究背景 1
1.2 研究動機與目的 2
第二章 研究原理 3
2.1 帕金森氏症 3
2.1.1 α-突觸核蛋白（Alpha-synuclein） 3
2.1.2 路易氏體（Lewy bodies） 3
2.1.3 神經傳導物質 4
2.1.4 皮質紋狀體-丘腦皮層迴路（CSTC loop） 4
2.1.5 腦–腸軸（Gut-brain axis） 6
2.2 腦深層電刺激 6
2.3 光刺激原理 7
2.3.1 光學生物窗 7
2.3.2 低功率光治療 8
2.4 腦波原理 9
第三章 實驗設計與架構 10
3.1 實驗設計 10
3.1.1 近紅外光刺激儀 10
3.1.2 實驗之前置準備 11
3.1.3 刺激位置與參數 11
3.1.4 旋轉木滾輪測試（Rota-rod Test） 12
3.2 實驗架構 12
3.2.1 前置作業 13
3.2.2 手術操作 13
3.2.3 量測實驗 15
3.2.4 數據分析 16
第四章 實驗結果與分析 18
4.1 旋轉木滾輪測試結果 18
4.1.1 控制組與光刺激組一 18
4.1.2 控制組與光刺激組二 20
4.2 腦波分析 22
4.2.1 光刺激組一 22
4.2.2 光刺激組二 24
4.3 實驗結果比較 26
4.3.1 旋轉木滾輪測試分析 26
4.3.2 腦波結果分析 28
第五章 討論與總結 29
5.1 腦波實驗誤差討論 29
5.2 總結 32
參考文獻 33

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