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研究生:陳德軒
研究生(外文):Chen, De-Hsuan
論文名稱:高速數位影像之甲壁微循環檢查鏡應用於甲壁微血管血流速測量和評估
論文名稱(外文):High Speed Digital Video Capillaroscopy for Nail-fold Capillaries Blood Cell Velocity Measurement and Evaluation
指導教授:林顯豐
指導教授(外文):Lin, Shien-Fong
口試委員:羅佩禎張家齊
口試委員(外文):Luo, Pei-JenChang, Chia-Chi
口試日期:2019-02-22
學位類別:碩士
校院名稱:國立交通大學
系所名稱:生醫工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:英文
論文頁數:87
中文關鍵詞:微循環系統甲壁微循環顯微鏡甲壁床微血管
外文關鍵詞:microcirculationNail-fold capillaroscopyNail-fold capillaries
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微循環系統稱為人體第二個心臟,也是判斷身體健康狀況及疾病前兆的重要指標區域。在本研究中我們採用甲壁微循環檢查顯微鏡觀察人體指甲甲壁床區域的微血管型態
,發現健康的微血管是呈現類似髮夾形狀,而較不健康的微血管則是呈現不規則、麻花捲或樹枝型等型態,從這些微血管外觀就能初步判斷身體可能是處於亞健康狀態或是一些潛在疾病的前兆,這都是需要進一步地做觀察分析。
甲壁微循環檢查顯微鏡是一個檢查微血管相當成熟的技術,其優點是以非侵入式的方式觀測且方便快速,但使用前必須先塗上一層香柏油來增加甲壁層的透明度,才能進行微血管觀察。在本研究中我們也解決了原先手指自主抖動而造成視頻模糊的問題,我們使用軟性且有彈性泡棉當作是固定手指的機構,結果發現軟性泡棉不僅不會壓迫手指造成血流速變慢的情況且能大幅提升影像的穩定度。
我們同時也開發了一套能自動跟蹤紅血球移動速度的使用者介面,它主要功能是能自動跟蹤經過影像重建過後的原始視頻中的紅血球瞬時血流速度及運動軌跡,並用不同顏色區分血流速度的表示範圍,在實驗過程中我們使用兩種方式評估自動跟蹤技術的確校,分別是分析健康受測者的健康微血管及各類不健康微血管血流速的分佈趨勢,另一個則是利用60 mmHg的壓脈帶壓力測試(靜脈束縛)來判斷在束縛期間血流速度的變化趨勢,而我們從十位健康男性受測者的左手無名指取出甲壁微血管樣本視頻,最後結果發現其中4號與9號受測者的血流速低於正常水平值,而實驗前所測得的血壓值為135 / 68 mmHg與 134 / 65 mmHg皆有偏高的頃向,其他受測者也有血壓越高血流速度則越低的趨勢,原因可能跟個體體質因素及全身血管阻力變高而影響甲壁微血管的血流速度(低於正常水平),最後則迫使心臟收縮壓力必須提升(血壓變高)讓血流速回復趨近於正常水平,有研究指出甲壁微循環的血壓與血流速是成反比關係[26,41],我們不排除觀測甲壁微血管型態及血流速度可以作為初步判斷原發性高血壓前兆的重要指標。最後期望本技術未來能運用在檢測及預防臨床(糖尿病或原發性高血壓)等慢性疾病上。
The microcirculation system is called the second heart of the human body and is also an important indicator area for judge human health and disease precursors. This study used nail-fold capillaroscopy to observe the shape of the human capillaries and analyze the red blood cell (RBC) velocity. Finally, we developed "automatically track" multiple RBC GUI systems that automatically analyze the velocity and motion paths in capillaries. This system can display the results of capillaries RBC velocity in different shapes in real time.
In the use of nail-fold capillaroscopy, it is observed that there is a problem of video blurring caused by finger shake. In this study, we designed a soft and flexible material that stabilizes the finger to achieve optimal stability of the nail-fold capillary video. Finally, during the experiment we recruited ten healthy male volunteers. Before the start of the experiment, sit in a ventilated and constant temperature room for 5 minutes and record the blood pressure information. And we use two methods of analysis. The first is to analyze the different shape of the nail-fold capillaries RBC velocity and the motion path and observe the distribution trend of RBC velocity in different shapes of capillaries.
The second is a 60 mmHg cuff pressure test to observe the change trend of RBC velocity during pressure binding. The results showed that the RBC velocity of volunteers with higher blood pressure had a lower trend. Other volunteers have also been observed to have an inverse relationship between blood pressure and RBC velocity. Finally, we don’t rule out that this may be an important indicator of the symptoms of hypertension.
Contents
Contents............................................................................................................... I
List of figures ....................................................................................................III
List of tables .................................................................................................... VII
Acknowledgement ......................................................................................... VIII
Abstract in Chinese ......................................................................................... IX
Abstract.............................................................................................................. X Chapter 1 Introduction
1.1 Background ............................................................................................ 1
1.2 Motivation ...............................................................................................2
1.3 Objective..................................................................................................6
Chapter 2 Basic theory
2.1 Nail-fold capillaroscopy.........................................................................8
2.2 Principle of image processing.............................................................11
Chapter 3 Materials and Methods
3.1 Materials...............................................................................................19
3.2 Calculate the RBC velocity method evaluation..................................25
3.2.1 Double window method..........................................................25
3.2.2 Manual tracking of RBC method....................................27
3.2.3 Automatic tracking of RBC method...............................28
Chapter 4 Results and discussion
4.1 Capillary type analysis...........................................................................33
4.2 Cuff pressure restraint test....................................................................67
Chapter 5 Conclusion and future work............................................81
Reference........................................................................................................ 83
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