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研究生:陳柔云
研究生(外文):Chen, Jou-Yun
論文名稱:應用分子標記管理台灣黑鯛的增殖放流
論文名稱(外文):Genetic management of stock enhancement programs in black seabream (Acanthopagrus schlegelii) by using molecular markers
指導教授:黃章文
指導教授(外文):Huang, Chang-Wen
口試委員:徐德華潘婕玉黃章文
口試委員(外文):Hsu, Te-HuaPan, Chieh-YuHuang, Chang-Wen
口試日期:2019-01-11
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:中文
論文頁數:67
中文關鍵詞:資源放流微衛星標記遺傳多樣性養殖Acanthopagrus schlegelii
外文關鍵詞:Stock enhancementMicrosatellitesgenetic diversityAquacultureAcanthopagrus schlegelii
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黑鯛是台灣資源放流的重要對象。為擬定黑鯛放流的遺傳管理策略,我們收集了2批放流種魚場、2批放流樣本、4個野生族群(苗栗、雲林、嘉義及澎湖),及7個養殖族群與1個日本野生族群(九州)並以9組微衛星標記進行分析。在16個族群中(N= 669),大部分族群皆顯現較高的預期雜合度(He= 0.612-0.729),而放流種魚場及放流樣本均顯示較高的遺傳多樣性(Na= 9.6, 8.0和9.7; He= 0.724, 0.708和0.729),並且與台灣野生族群具有較小的分化指數(FST= 0.003-0.019)。僅管部分養殖場有較低的等位基因數(Na= 4.7),但若放流良好遺傳組成的黑鯛時,應不會對野生族群造成遺傳上的改變。我們建議未來以等位基因數(Na)大於8、預期雜合度(He)大於0.7做為遺傳多樣性的指標。
Black seabream (Acanthopagrus schlegelii) is an important commercial and sport fishing species inhabiting Taiwan coastal waters, where an intensive stock enhancement program is carried out for this species. To clarify the fine-scale genetic effects of stock enhancement, we collected and examined samples from the stock hatchery (2015 and 2016) and two releases using nine microsatellite markers. Furthermore, specimens from four locations from wild (Miaoli, Yunlin, Chiayi, and Penghu), seven from commercial hatcheries and one wild population from Kyushu, Japan were used for comparison. High genetic diversity was observed among 16 locations (N= 669, He= 0.612-0.729). The stock hatchery (2016), and two releases showed higher genetic diversity (Na= 9.6, 8.0 and 9.7; He= 0.724, 0.708 and 0.729) and lower pairwise FST value (0.003-0.019) compared to four locations from wild. It suggested that well-designed stock enhancement won’t result in a further decrease of genetic diversity. However, lower number of alleles (Na= 4.7) was still found at some private hatchery. It is necessary to monitor genetic variation between hatchery and target wild populations for stock enhancement program. We suggest the standard values of genetic diversity for stock enhancement are Na > 8 and He > 0.7.
謝辭 I
中文摘要 II
英文摘要 IV
目錄 VI
圖目錄 VIII
表目錄 IX
第一節、前言 1
1. 黑鯛(Acanthopagrus schlegelii)簡介 1
2. 台灣的資源放流 1
3. 遺傳標記簡介 3
4. 研究目的 5
第二節、材料方法 6
1. 樣本來源及採集地點 6
2. 萃取樣本基因組DNA 6
3. 多重微衛星DNA的擴增 7
4. 微衛星DNA的篩選及優化 8
5. 數據分析 9
第三節、結果 11
1. 微衛星標記的組合 11
2. 微衛星DNA標誌多樣性參數 12
3. 分析微衛星DNA標誌基因窄化 12
4 檢測樣本數與等位基因數模擬分析 13
第四節、討論 15
1. 分子標記應用於標記放流的比較 15
2. 標記放流的遺傳管理策略 15
3. 使用分子標記輔助種苗放流的效益 17
4. 分子標記進行種原鑑別的原理及限制 17
5. DNA分子標記應用於個體來源判別的基本原理 18
第五節、未來展望 22
參考文獻 23
圖 27
表 42
附表 46
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