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研究生:陳詩瑤
研究生(外文):Shiyao Chen
論文名稱:評估臺灣紫芝萃取物抗PM2.5誘導ROS之潛力
論文名稱(外文):Evaluation of Potential Protective Effect of Ganoderma formosanum Extract Against PM2.5-Induced ROS Generation
指導教授:鄭光成鄭光成引用關係
指導教授(外文):Kuan-Chen Cheng
口試委員:陳錦樹蔡宗佑楊麗嬋許仁駿
口試委員(外文):Chin-Shuh ChenTsung-Yu TsaiLi-Chan YangRen-Jun Hsu
口試日期:2019-07-26
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:食品科技研究所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:中文
論文頁數:104
中文關鍵詞:臺灣紫芝PM2.5ROS抗氧化
DOI:10.6342/NTU201903267
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臺灣紫芝 (Ganoderma formosanum) 是臺灣特有種靈芝,其萃取物具有多種生理活性。本研究通過液態發酵的方式來大量生產臺灣紫芝菌絲體,以95%酒精對其菌絲體進行萃取,並利用人類皮膚角質細胞 (HaCaT cell) 探討經液態發酵之菌絲體萃取物 (G. formosanum mycelial extract, GFE) 對PM2.5 (fine particulate matter) 所引起之活性含氧物生成以及細胞傷害之調控功能,並進一步利用斑馬魚動物模型證實PM2.5對於動物體的傷害。傷害。
研究結果顯示500 μg/mLppm的PM2.5會導致胞內ROS (reactive oxygen species) 增加2.7倍且導致細胞存活率下降45%,且具有dose-dependent效應;而在斑馬魚體內也可以觀察到相似的效應,2,000 μg/mLppm的PM2.5會導致斑馬魚體內的ROS增加1.7倍,且會引起斑馬魚心率下降;加入臺灣紫芝萃取物處理後,PM2.5所引起的胞內ROS的水平顯著減少59%,同時減少30%的細胞凋亡;在斑馬魚動物模型中,經臺灣紫芝萃取物處理後斑馬魚體內之ROS水平降低了67%。以流式細胞儀分析也顯示臺灣紫芝萃取物之預處理可以有效減少細胞凋亡。綜上所述,臺灣紫芝萃取物可透過抗氧化活性從而保護皮膚細胞不受PM2.5損傷。
Ganoderma formosanum is an endemic species of Ganoderma in Taiwan and extract of G. formosanum possesses diverse bioactivities. This study discussed about the potential protective effect of submerged cultivated G. formosanum mycelial extract (GFE) against PM2.5-induced ROS generation and cell viability in human keratinocyte (HaCaT cell) model, meanwhile assessed the toxicity of PM2.5 on zebrafish in vivo model.
Results showed that PM2.5 treatment promoted both the intracellular ROS level and decreased cell viability in a dose-dependent manner. The similar effect could also be observed in zebrafish model. Pretreatment of G. formosanum extract ameliorated intracellular ROS generation and apoptosis. The PM2.5-induced cell damage was attenuated by antioxidant N-acetyl cysteine, confirming that PM2.5 cellular toxicity was due to oxidative stress. Taken together, these findings implied that antioxidant activities of G. formosanum extract might play an important role in the protection of PM2.5-induced cell damage.
目錄
謝誌 I
摘要 III
Abstract IV
目錄 VI
圖目錄 IX
表目錄 XI
List of Figures XII
List of Tables XIV
壹、前言 1
貳、文獻回顧 3
一、靈芝 3
1.1 靈芝的種類 3
1.2 靈芝的生理活性 5
1.3 靈芝的活性成分 5
二、臺灣紫芝 9
三、靈芝培養方式 12
3.1 靈芝固態培養 12
3.2 靈芝菌液態培養 13
四、空氣污染 13
4.1 粒狀物質 (particulate matter, PM) 15
4.2 粒狀物質的組成 18
4.3 對粒狀物質的限制 18
4.4 粒狀物質對皮膚之傷害 19
4.5 粒狀物質對細胞的影響 21
五、斑馬魚動物模型 23
六、活性含氧物 (Reactive oxygen species) 25
七、研究目的 27
參、實驗架構 29
肆、材料與方法 32
一、臺灣紫芝液態發酵生產 32
1.1 實驗藥品 32
1.2 儀器設備 33
1.3 實驗菌株 34
1.4 培養基 34
1.5 培養方法 35
1.6 各活性劃分之分離 36
二、臺灣紫芝各活性劃分用於曝露於粒狀物質之皮膚細胞存活率測定 37
2.1 實驗藥品 37
2.2 儀器設備 38
2.3 實驗細胞株 38
2.4 細胞培養基 39
2.5 細胞培養 40
2.6 細胞存活率之測定 42
2.7 細胞胞內ROS之測定 42
2.8 PM2.5的製備 43
三、萃取物各活性劃分之活性探討 43
3.1各活性劃分用於曝露於PM2.5之皮膚細胞存活率測定 43
3.2各活性劃分用於曝露於PM2.5之皮膚細胞胞內ROS之測定 45
3.3 流式細胞儀分析細胞凋亡相關表現 45
四、斑馬魚培養 48
4.1 培養方法 48
4.2 斑馬魚體內ROS測定 48
五、統計分析 49
伍、結果與討論 50
一、PM2.5之形態 50
二、PM2.5之毒性 50
2.1對細胞存活率之影響 50
2.2對胞內ROS之影響 53
2.3 對斑馬魚之影響 55
2.4 PM2.5所引起之氧化壓力 56
三、臺灣紫芝萃取物抗PM2.5之活性探討 59
3.1臺灣紫芝萃取物抗PM2.5活性之初篩 59
3.2臺灣紫芝萃取物之毒性探討 59
3.3 臺灣紫芝萃取物各活性劃分抗PM2.5之活性探討 62
四、流式細胞儀分析細胞凋亡 67
4.1 PM2.5所造成的傷害 67
4.2 臺灣紫芝萃取物的保護效果 67
陸、結論與未來展望 72
柒、參考文獻 73
捌、附錄 XV
附錄一 XV
履歷表 XVIII
附錄二 XIX
水野卓 & 川合正允,1997,菇類的化學,生化學(初版),臺北市: 編譯館。
王伯徹,2005,食藥用菇菌類保健食品產業發展彙編(第一版)。 Essays on Health Food Industry of Edible and Medicinal Mushrooms,南投市: 行政院農業委員會農糧署出版。
王君森 & 劉志俊,2016,基於 ITS2 的臺灣靈芝物種鑑定。 Journal of Information Technology and Applications (資訊科技與應用期刊), 10(2), 12-30.
吳亭瑤,2001,靈芝,,妙不可言: : 穿梭2500年的養生良方(初版)。 Healing with Ganoderma. 台北市: 自然風文化出版。
林志彬,2007,靈芝的現代研究(第三版),北京市:北京大學醫學出版社。
林志彬,2008,靈芝:從神奇到科學(第一版),北京市: 北京大學醫學出版社。
陳明煦,2006,臺灣紫芝生物活性代謝產物之醱酵生產與功能評估。
許瑞祥,2010,2010靈芝概論(再版),臺北市: 年喜文教基金會。
劉育姍,康瑋帆,呂昀陞 & 石信德,2016,我國菇類產業現況與技術發展策略分析。
Andreau, K., Leroux, M., & Bouharrour, A. (2012). Health and cellular impacts of air pollutants: from cytoprotection to cytotoxicity. Biochemistry Research International, 2012.
Apel, K., & Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373-399.
Ascenso, A., & Marques, H. C. (2009). Acne in the adult. Mini Reviews in Medicinal Chemistry, 9(1), 1-10.
Ashkenazi, A. (2008). Directing cancer cells to self-destruct with pro-apoptotic receptor agonists. Nature Reviews Drug Discovery, 7(12), 1001.
Bell, M. L., Dominici, F., Ebisu, K., Zeger, S. L., & Samet, J. M. (2007). Spatial and temporal variation in PM2.5 chemical composition in the United States for health effects studies. Environmental Health Perspectives, 115(7), 989-995.
Brook, R. D., Franklin, B., Cascio, W., Hong, Y., Howard, G., Lipsett, M., Luepker R., Mittleman M., Samet J., SmithJr S. C., & . . . Tager I.Smith Jr, S. C. (2004). Air pollution and cardiovascular disease: a statement for healthcare professionals from the Expert Panel on Population and Prevention Science of the American Heart Association. Circulation, 109(21), 2655-2671.
Cao, Y., Wu, S.-H., & Dai, Y.-C. (2012). Species clarification of the prize medicinal Ganoderma mushroom “Lingzhi”. Fungal Diversity, 56(1), 49-62.
Chang, M.-Y., Tsai, G.-J., & Houng, J.-Y. (2006). Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enzyme and Microbial Technology, 38(3-4), 407-414.
Chang, T. T., & Chen, T. (1984). Ganoderma formosanum sp. nov. on Formosan sweet gum in Taiwan. Transactions of the British Mycological Society, 82(4), 731-733.
Chen, H. (2013). Modern solid state fermentation. Netherlands: Springer.
Choi, T. Y., Kim, J. H., Ko, D. H., Kim, C. H., Hwang, J. S., Ahn, S., Kim S.Y., Kim C.-D., LEE J.-H., & . . . Yoon, T. J. (2007). Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds. Pigment Cell Research, 20(2), 120-127.
D''Costa, A., & Shepherd, I. T. (2009). Zebrafish development and genetics: introducing undergraduates to developmental biology and genetics in a large introductory laboratory class. Zebrafish, 6(2), 169-177.
Dumitrescu, E., Karunaratne, D. P., Prochaska, M. K., Liu, X., Wallace, K. N., & Andreescu, S. (2017). Developmental toxicity of glycine-coated silica nanoparticles in embryonic zebrafish. Environmental Pollution, 229, 439-447.
Ghobrial, I. M., Witzig, T. E., & Adjei, A. A. (2005). Targeting apoptosis pathways in cancer therapy. CA: A Cancer Journal for Clinicians, 55(3), 178-194.
Gill, B. S., & Kumar, S. (2016). Ganoderic acid targeting multiple receptors in cancer: in silico and in vitro study. Tumor Biology, 37(10), 14271-14290.
Gill, B. S., & Kumar, S. (2017). Ganoderic acid modulating TNF and its receptors: in silico and in vitro study. Medicinal Chemistry Research, 26(6), 1336-1348.
Gill, B. S., Mehra, R., Kumar, V., & Kumar, S. (2017). Ganoderic acid, lanostanoid triterpene: a key player in apoptosis. Investigational New Drugs, 1-8.
Guaita, R., Pichiule, M., Maté, T., Linares, C., & Díaz, J. (2011). Short-term impact of particulate matter (PM2.5) on respiratory mortality in Madrid. International Journal of Environmental Health Research, 21(4), 260-274.
Haigis, M. C., & Yankner, B. A. (2010). The aging stress response. Molecular Cell, 40(2), 333-344.
Held, P. (2012). An introduction to reactive oxygen species. Tech Resources-App Guides, 802, 5-9.
Hillaireau, H., & Couvreur, P. (2009). Nanocarriers’ entry into the cell: relevance to drug delivery. Cellular and Molecular Life Sciences, 66(17), 2873-2896.
Hiura, T. S., Li, N., Kaplan, R., Horwitz, M., Seagrave, J.-C., & Nel, A. E. (2000). The role of a mitochondrial pathway in the induction of apoptosis by chemicals extracted from diesel exhaust particles. The Journal of Immunology, 165(5), 2703-2711.
Hsu, K.-D., Chen, H.-J., Wang, C.-S., Lum, C.-C., Wu, S.-P., Lin, S.-P., & Cheng, K.-C. (2016). Extract of Ganoderma formosanum mycelium as a highly potent tyrosinase inhibitor. Scientific Reports, 6, 32854.
Hsu, K.-D., Wu, S.-P., Lin, S.-P., Lum, C.-C., & Cheng, K.-C. (2017). Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling. Journal of Food and Drug Analysis, 25(4), 804-811.
Hsu, P.-L., Lin, Y.-C., Ni, H., & Mo, F.-E. (2018). Ganoderma Triterpenoids Exert Antiatherogenic Effects in Mice by Alleviating Disturbed Flow-Induced Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity, 2018.
Hu, R., Xie, X.-Y., Xu, S.-K., Wang, Y.-N., Jiang, M., Wen, L.-R., . . . Guan, L. (2017). PM2.5 exposure elicits oxidative stress responses and mitochondrial apoptosis pathway activation in HaCaT keratinocytes. Chinese Medical Journal, 130(18), 2205.
Huang, S., Mao, J., Ding, K., Zhou, Y., Zeng, X., Yang, W., . . . Xia, P. (2017). Polysaccharides from Ganoderma lucidum promote cognitive function and neural progenitor proliferation in mouse model of Alzheimer''s disease. Stem Cell Reports, 8(1), 84-94.
Hüseyin, Ö., Sevgi, İ., Engin, D., Fırat, A., Gülsüm, P., & Şenay, D. (2017). Ganoderma lucidum Protects Rat Brain Tissue Against Trauma-Induced Oxidative Stress. Korean Journal of Neurotrauma, 13(2), 76-84.
Hyun, Y. J., Piao, M. J., Kang, K. A., Zhen, A. X., Fernando, M., Sameera, P. D., . . . Hyun, J. W. (2019). Effect of Fermented Fish Oil on Fine Particulate Matter-Induced Skin Aging. Marine Drugs, 17(1), 61.
Kerr, J. F., Wyllie, A. H., & Currie, A. R. (1972). Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics. British Journal of Cancer, 26(4), 239.
Kim, J., Kim, E.-H., Oh, I., Jung, K., Han, Y., Cheong, H.-K., & Ahn, K. (2013). Symptoms of atopic dermatitis are influenced by outdoor air pollution. Journal of Allergy and Clinical Immunology, 132(2), 495-498. e491.
Kim, K.-H., Kabir, E., & Kabir, S. (2015). A review on the human health impact of airborne particulate matter. Environment International, 74, 136-143.
Kim, K. E., Cho, D., & Park, H. J. (2016). Air pollution and skin diseases: adverse effects of airborne particulate matter on various skin diseases. Life Sciences, 152, 126-134.
Ko, F. W., & Hui, D. S. (2012). Air pollution and chronic obstructive pulmonary disease. Respirology, 17(3), 395-401.
Kubota, T., Asaka, Y., Miura, I., & Mori, H. (1982). Structures of Ganoderic Acid A and B, Two New Lanostane Type Bitter Triterpenes from Ganoderma lucidum (FR.) KARST. Helvetica Chimica Acta, 65(2), 611-619.
Kyung, O. Y., Grabinski, C. M., Schrand, A. M., Murdock, R. C., Wang, W., Gu, B., . . . Hussain, S. M. (2009). Toxicity of amorphous silica nanoparticles in mouse keratinocytes. Journal of Nanoparticle Research, 11(1), 15-24.
Lademann, J., Schaefer, H., Otberg, N., Teichmann, A., Blume-Peytavi, U., & Sterry, W. (2004). Penetration von Mikropartikeln in die menschliche HautPenetration of microparticles into human skin. Der Hautarzt, 55(12), 1117-1119.
Lee, C.-W., Lin, Z.-C., Hsu, L.-F., Fang, J.-Y., Chiang, Y.-C., Tsai, M.-H., . . . Lee, I.-T. (2016). Eupafolin ameliorates COX-2 expression and PGE2 production in particulate pollutants-exposed human keratinocytes through ROS/MAPKs pathways. Journal of Ethnopharmacology, 189, 300-309.
Lee, H., Jung, K.-H., Park, S., Kil, Y.-S., Chung, E. Y., Jang, Y. P., . . . Bae, H. (2014). Inhibitory effects of Stemona tuberosa on lung inflammation in a subacute cigarette smoke-induced mouse model. BMC Complementary and Alternative Medicine, 14(1), 513.
Levine, B., & Kroemer, G. (2008). Autophagy in the pathogenesis of disease. Cell, 132(1), 27-42.
Li, C.-H., Chen, P.-Y., Chang, U.-M., Kan, L.-S., Fang, W.-H., Tsai, K.-S., & Lin, S.-B. (2005). Ganoderic acid X, a lanostanoid triterpene, inhibits topoisomerases and induces apoptosis of cancer cells. Life Sciences, 77(3), 252-265.
Li, W., Nie, S., Chen, Y., Wang, Y., Li, C., & Xie, M. (2011). Enhancement of cyclophosphamide-induced antitumor effect by a novel polysaccharide from Ganoderma atrum in sarcoma 180-bearing mice. Journal of Agricultural and Food Chemistry, 59(8), 3707-3716.
Li, W.-J., Li, L., Zhen, W.-Y., Wang, L.-F., Pan, M., Lv, J.-Q., . . . Xie, M.-Y. (2017). Ganoderma atrum polysaccharide ameliorates ROS generation and apoptosis in spleen and thymus of immunosuppressed mice. Food and Chemical Toxicology, 99, 199-208.
Li, W.-J., Nie, S.-P., Yao, Y.-F., Liu, X.-Z., Shao, D.-Y., Gong, D.-M., . . . Xie, M.-Y. (2015). Ganoderma atrum polysaccharide ameliorates hyperglycemia-induced endothelial cell death via a mitochondria-ROS pathway. Journal of Agricultural and Food Chemistry, 63(37), 8182-8191.
Liao, B., Chen, X., Han, J., Dan, Y., Wang, L., Jiao, W., . . . Chen, S. (2015). Identification of commercial Ganoderma (Lingzhi) species by ITS2 sequences. Chinese Medicine, 10(1), 22.
Lin, J.-M., Lin, C.-C., Chen, M.-F., Ujiie, T., & Takada, A. (1995). Radical scavenger and antihepatotoxic activity of Ganoderma formosanum, Ganoderma lucidum and Ganoderma neo-japonicum. Journal of Ethnopharmacology, 47(1), 33-41.
Liu, R.-M., & Zhong, J.-J. (2011). Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. Phytomedicine, 18(5), 349-355.
Lo, Y.-C., Chien, S.-C., Mishchuk, D. O., Slupsky, C. M., & Mau, J.-L. (2016). Quantification of water-soluble metabolites in medicinal mushrooms using proton NMR spectroscopy. International Journal of Medicinal Mushrooms, 18(5).
Mannucci, P. M., Harari, S., Martinelli, I., & Franchini, M. (2015). Effects on health of air pollution: a narrative review. Internal and Emergency Medicine, 10(6), 657-662.
Martinet, W., & De Meyer, G. R. (2009). Autophagy in atherosclerosis: a cell survival and death phenomenon with therapeutic potential. Circulation Research, 104(3), 304-317.
Mathew, R., Karantza-Wadsworth, V., & White, E. (2007). Role of autophagy in cancer. Nature Reviews Cancer, 7(12), 961.
McGrath, P., & Li, C.-Q. (2008). Zebrafish: a predictive model for assessing drug-induced toxicity. Drug Discovery Today, 13(9-10), 394-401.
Mehta, S., Shin, H., Burnett, R., North, T., & Cohen, A. J. (2013). Ambient particulate air pollution and acute lower respiratory infections: a systematic review and implications for estimating the global burden of disease. Air Quality, Atmosphere & Health, 6(1), 69-83.
Mokhtari, V., Afsharian, P., Shahhoseini, M., Kalantar, S. M., & Moini, A. (2017). A review on various uses of N-acetyl cysteine. Cell Journal (Yakhteh), 19(1), 11.
Nishida, K., Yamaguchi, O., & Otsu, K. (2008). Crosstalk between autophagy and apoptosis in heart disease. Circulation Research, 103(4), 343-351.
Nixon, R. A. (2013). The role of autophagy in neurodegenerative disease. Nature Medicine, 19(8), 983.
Pandey, A. (2003). Solid-state fermentation. Biochemical Engineering Journal, 13(2-3), 81-84.
Paravicini, T. M., & Touyz, R. M. (2006). Redox signaling in hypertension. Cardiovascular Research, 71(2), 247-258.
Park, Y.-J., Kwon, O., Son, E.-S., Yoon, D.-E., Han, W., Yoo, Y.-B., & Lee, C.-S. (2012). Taxonomy of Ganoderma lucidum from Korea based on rDNA and partial β-tubulin gene sequence analysis. Mycobiology, 40(1), 71-75.
Park, Y.-J., Kwon, O.-C., Son, E.-S., Yoon, D.-E., Han, W., Nam, J.-Y., . . . Lee, C.-S. (2012). Genetic diversity analysis of Ganoderma species and development of a specific marker for identification of medicinal mushroom Ganoderma lucidum. African Journal of Microbiology Research, 6(25), 5417-5425.
Pi, C.-C., Chu, C.-L., Lu, C.-Y., Zhuang, Y.-J., Wang, C.-L., Yu, Y.-H., . . . Chen, C.-J. (2014). Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo. Vaccine, 32(3), 401-408.
Piao, M. J., Ahn, M. J., Kang, K. A., Ryu, Y. S., Hyun, Y. J., Shilnikova, K., . . . Kang, H. K. (2018). Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis. Archives of Toxicology, 1-15.
Pierdominici, M., Maselli, A., Cecchetti, S., Tinari, A., Mastrofrancesco, A., Alfè, M., . . . Carpinelli, G. (2014). Diesel exhaust particle exposure in vitro impacts T lymphocyte phenotype and function. Particle and Fibre Toxicology, 11(1), 74.
Portt, L., Norman, G., Clapp, C., Greenwood, M., & Greenwood, M. T. (2011). Anti-apoptosis and cell survival: a review. Biochimica et Biophysica Acta (BBA). Molecular Cell Research, 1813(1), 238-259.
Postemsky, P. D., Bidegain, M. A., González-Matute, R., Figlas, N. D., & Cubitto, M. A. (2017). Pilot-scale bioconversion of rice and sunflower agro-residues into medicinal mushrooms and laccase enzymes through solid-state fermentation with Ganoderma lucidum. Bioresource Technology, 231, 85-93.
Puntoni, R., Ceppi, M., Gennaro, V., Ugolini, D., Puntoni, M., La Manna, G., . . . Merlo, D. F. (2004). Occupational exposure to carbon black and risk of cancer. Cancer Causes & Control, 15(5), 511-516.
Sagai, M., & Ichinose, T. (2001). Health effects of diesel exhaust and diesel exhaust particles. Oxidative Stress and Disease, 7, 157-184.
Shukla, V., Mishra, S. K., & Pant, H. C. (2011). Oxidative stress in neurodegeneration. Advances in Pharmacological Sciences, 2011.
Smina, T., Nitha, B., Devasagayam, T., & Janardhanan, K. (2017). Ganoderma lucidum total triterpenes induce apoptosis in MCF-7 cells and attenuate DMBA induced mammary and skin carcinomas in experimental animals. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 813, 45-51.
Stern, S. T., Adiseshaiah, P. P., & Crist, R. M. (2012). Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity. Particle and Fibre Toxicology, 9(1), 20.

Su, C., Tang, C., Zhang, J., Chen, M., & Pan, Y. (2007). The phylogenetic relationship of cultivated isolates of Ganoderma in China inferred from nuclear ribosomal DNA ITS sequences. Acta Microbiologica Sinica, 47(1), 11-16.
Su, L., Liu, L., Jia, Y., Lei, L., Liu, J., Zhu, S., . . . Yang, B. (2017). Ganoderma triterpenes retard renal cyst development by downregulating Ras/MAPK signaling and promoting cell differentiation. Kidney International, 92(6), 1404-1418.
Trachootham, D., Alexandre, J., & Huang, P. (2009). Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach. Nature Reviews Drug Discovery, 8(7), 579.
Tseng, C.-Y., Chung, M.-C., Wang, J.-S., Chang, Y.-J., Chang, J.-F., Lin, C.-H., . . . Chao, M.-W. (2016). Potent In Vitro Protection Against PM 2. 5-Caused ROS Generation and Vascular Permeability by Long-Term Pretreatment with Ganoderma tsugae. The American Journal of Chinese Medicine, 44(02), 355-376.
Tzivian, L., Winkler, A., Dlugaj, M., Schikowski, T., Vossoughi, M., Fuks, K., . . . Hoffmann, B. (2015). Effect of long-term outdoor air pollution and noise on cognitive and psychological functions in adults. International Journal of Hygiene and Environmental Health, 218(1), 1-11.
van Voorhis, M., Knopp, S., Julliard, W., Fechner, J. H., Zhang, X., Schauer, J. J., & Mezrich, J. D. (2013). Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor. PloS One, 8(12), e82545.
Vierkötter, A., Schikowski, T., Ranft, U., Sugiri, D., Matsui, M., Krämer, U., & Krutmann, J. (2010). Airborne particle exposure and extrinsic skin aging. Journal of Investigative Dermatology, 130(12), 2719-2726.
Wang, D.-M., & Yao, Y.-J. (2005). Intrastrain internal transcribed spacer heterogeneity in Ganoderma species. Canadian Journal of Microbiology, 51(2), 113-121.
Wang, X.-C., Xi, R.-J., Li, Y., Wang, D.-M., & Yao, Y.-J. (2012). The species identity of the widely cultivated Ganoderma,‘G. lucidum’(Ling-zhi), in China. PloS One, 7(7), e40857.
Wu, G.-S., Lu, J.-J., Guo, J.-J., Li, Y.-B., Tan, W., Dang, Y.-Y., . . . Wang, Y.-T. (2012). Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells. Fitoterapia, 83(2), 408-414.
Xia, Q., Zhang, H., Sun, X., Zhao, H., Wu, L., Zhu, D., . . . Mao, X. (2014). A comprehensive review of the structure elucidation and biological activity of triterpenoids from Ganoderma spp. Molecules, 19(11), 17478-17535.
Yin, L., Niu, Z., Chen, X., Chen, J., Zhang, F., & Xu, L. (2014). Characteristics of water-soluable inorganic ions in PM2.5 and PM2.5-10 in the urban agglomeration along the Western Taiwan Strait Region, China. Environmental Science and Pollution Research, 21(7), 5141-5156.
Yue, Q.-X., Cao, Z.-W., Guan, S.-H., Liu, X.-H., Tao, L., Wu, W.-Y., . . . Guo, D.-A. (2008). Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network. Molecular & Cellular Proteomics, 7(5), 949-961.
Yuk, J.-M., Yoshimori, T., & Jo, E.-K. (2012). Autophagy and bacterial infectious diseases. Experimental & Molecular Medicine, 44(2), 99.
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J., . . . Ding, S. (2017). Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging. Molecular Medicine Reports, 15(1), 111-116.
Zhang, Y., Li, S., Li, J., Han, L., He, Q., Wang, R., . . . Liu, K. (2018). Developmental toxicity induced by PM2.5 through endoplasmic reticulum stress and autophagy pathway in zebrafish embryos. Chemosphere, 197, 611-621.
Zhang, Y.-S., Li, W.-J., Zhang, X.-Y., Yan, Y.-X., Nie, S.-P., Gong, D.-M., . . . Xie, M.-Y. (2017). Ganoderma atrum polysaccharide ameliorates anoxia/reoxygenation-mediated oxidative stress and apoptosis in human umbilical vein endothelial cells. International Journal of Biological Macromolecules, 98, 398-406.
Zhao, H., & Luo, Y. (2017). Traditional Chinese Medicine and Aging Intervention. Aging and Disease, 8(6), 688.
Zhu, X.-L., Chen, A.-F., & Lin, Z.-B. (2007). Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. Journal of Ethnopharmacology, 111(2), 219-226.
Zou, Y., Zhang, Y., Han, L., He, Q., Hou, H., Han, J., . . . Liu, K. (2017). Oxidative stress‐mediated developmental toxicity induced by isoniazide in zebrafish embryos and larvae. Journal of Applied Toxicology, 37(7), 842-852.
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