臺灣博碩士論文加值系統

納米粒子作為治療和診斷劑，例如氧化釤，氧化钆和氧化鐵，其尺寸小於 20nm被包覆在
生物相容性聚合物像是聚多巴胺和藻酸鹽之中。目的是創造用於癌症治療球形或直徑約
20-40μm的治療型診斷生物材料。
一種簡便的方法，有經濟效率且環保地用於合成直徑約 250nm的聚多巴胺球體以及納
米粒子嵌入的聚多巴胺。關於金屬氧化物@聚多巴胺，利用水和乙醇的混合物（70％/
30％（V/V））含有分散的納米顆粒，然後加入一定量的多巴胺鹽酸鹽，並在 15分鐘內
攪拌稀釋。氨水溶液產生鹼性環境，導致形成黑色且水分散性的金屬氧化物@聚多巴胺複
合材料。另一方面，研究了溶劑體積，pH和單體濃度作為多巴胺鹽酸鹽對生物材料的影
響及其形態利用 TEM-SEM進行調查。最佳生物材料三金屬氧化物包覆在聚多巴胺中通過
STEM數據得知為球形且很小。
對於金屬氧化物@藻酸鹽@聚多巴胺，光學顯微鏡和動態光散射數據表明，藻酸鹽經
由超聲波處理得到了微米尺度。因此，利用能量（瓦特），藻酸鹽濃度或鈣離子濃度來研
究尺寸的變化。然而，超聲能量影響導致金屬氧化物藻酸鹽結構中的空腔不是球形的。，
因此，聚多巴胺塗層是必需的為了改善最佳生物材料的型態。單層塗佈或多層塗佈利用熱
重分析進行且定量。

Nanoparticles as therapeutic and diagnostic agents such as Samarium oxide, Gadolinium
oxide and Iron oxide got the size less than 20nm were encapsulated in biocompatible polymers, polydopamine and alginate. The purpose is to create the theranostics biomaterial either spherical or 20-40µm in diameter that utilized in cancer treatment.
A facile method, economic efficiency and eco-friendly, was applied to synthesis the
spheres of polydopamine around 250nm in diameter as well as nanoparticle-embedded
polydopamine. With respect to metal oxide@polydopamine, a mixture of water and ethanol as the ratio 70%/30% (volume/volume) contains dispersed nanoparticles followed by an amount of dopamine hydrochloride which added and stirred to dilute in 15 minutes. Ammonia solution created the alkaline ambience led to the formation of black water-dispersible metal oxide@polydopamine composite material. On the other hand, the influence of the volume of solvent, pH and concentration of monomer as dopamine hydrochloride in the morphology of biomaterial was investigated by TEM-SEM. The optimum biomaterial, tri-metal oxide encapsulated in polydopamine by STEM, obtained the spherical shape but the size is small.
In terms of metal oxide@alginate@polydopamine, the optical microscope and dynamic
light scattering data showed that alginate got the micrometer in size by the ultrasonic power. Herein, the energy (Watts), concentration of alginate or concentration of calcium ion was employed to investigate the change in size. However, the metal oxide@alginate wasn’t spherical because of the cavity in its structure owing to sonication energy. Hence, coating layer of polydopamine is necessary in improving the shape of optimum biomaterial. Single coating or multiple coating was conducted and quantified by thermogravitry analysis.

CONTENTS
CHAPTER 1-Introduction and Motivation 1
1.1. Introduction 1
1.1.1. Sm2O3- a therapeutic agent 1
1.1.2. Gd2O3 and Fe3O4- diagnostic agents 4
1.1.3. Polydopamine 6
1.1.4. Alginate 7
1.2. Motivation 10
CHAPTER 2-Experiemental section 12
2.1. Reagents 12
2.2. Synthesis of materials 12
2.2.1. Synthesis of nanoparticles 12
2.2.2. Synthesis of PDA and metal oxide@PDA 14
2.2.3. Synthesis of metal oxide@Alginate 15
2.2.4. Synthesis of metal oxide@alginate@PDA 15
2.3. Characterization 16
CHAPTER 3- Polydopamine and metal oxide@polydopamine 17
3.1. Polydopamine (PDA) 17
3.2. The core-shell metal oxide@PDA material 20
3.2.1. The volume of solution to the size and shape of Sm2O3@PDA 20
3.2.2. Effect of pH to the morphology of Sm2O3@PDA 22
3.2.3. The DA concentration influences the Sm2O3@PDA appearance 24
3.2.4. Gd2O3@PDA 26
3.2.5. Fe3O4@PDA 27
3.2.6. Sm2O3, Gd2O3 and Fe3O4 encapsulated by PDA 29
Chapter 4: Alginate and metal oxide@alginate@polydopamide 34
4.1. Alginate 34
4.1.1. The size vs ultrasonication energy 34
4.1.2. The size vs alginate concentration 36
4.1.3. The size vs CaCl2 concentration 38
4.2. Synthesize metal oxide@alginate@polydopamine 40
4.2.1. Single coating by PDA 40
4.2.2. Multiple coating by PDA 44
Chapter 5: Conclusions and recommendations 51
5.1. Conclusions 51
5.1.1. Metal oxide@PDA 51
5.1.2. Metal oxide@alginate@PDA 51
5.2. Recommendations 52
APPENDIX 58

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