Hippo 訊息傳導路徑 (The Hippo pathway) 是一條在許多物種間具高度保留性 的訊息傳導路徑，其功能在於調控細胞生長、細胞凋亡，使正確的組織器官能夠 形成。儘管 Hippo 訊息傳導路徑的主要核心因子，在先前的研究中已經被找出， 然而其他參與調控此條路徑的因子，仍有待研究透徹。
由於 Hippo 訊息傳導路徑的主要調控了轉錄共同活化子(transcriptional coactivator)──Yorkie (Yki) 的活化，為了找出其他調控Hippo pathway的因子， 我將研究重心擺在與 Yki 有物理性交互作用的蛋白質上，包括 Brm、Cpsf5 及 Cpsf6。由於 Hippo 訊息傳導路徑在卵子生成過程 (oogenesis) 調控了許多重要 的程序，為了探討這些蛋白質是否在 Hippo 訊息傳導路徑中扮演重要的角色，我 使用了果蠅的卵巢作為研究系統。
在本篇研究中，我發現抑制 brm 的表現會造成早期的 egg chamber 融合，這代 表在卵子生成過程的早期，形成正常的 egg chamber 是需要 brm 的。抑制 Cpsf5 或 Cpsf6 的表現會阻礙 border cells 的移動、增加 polar cell 以及 border cell 的數目， 並影響 M-E transition。另外，我也發現抑制了 hrg, Clp 以及 Cpsf160 的表現後， 會觀察到和抑制 Cpsf5 或 Cpsf6 相似的表現型。綜合以上結果，我的研究探討了 brm、Cpsf5 及 Cpsf6 在卵子生成過程中所扮演的角色。

The Hippo signaling pathway controls organ size by regulating cell proliferation and programmed cell death in a highly conserved manner from Drosophila to mammals. Although the core components of the Hippo pathway have been identified, regulators of the Hippo pathway remain incomplete. Since Yorkie (Yki) is a major effector of the Hippo pathway. To find more regulators of the Hippo pathway, I focused on Yki-interacting proteins, Brm, Cpsf5 and Cpsf6. To investigate whether they interact with Yki functionally, I used follicle cells in the Drosophila ovary as a model system because the Hippo pathway controls multiple steps of follicle cell differentiation. In this study, I found that knockdown of brm in follicle cell precursors resulted in egg chamber fusion at early stages, indicating that brm is required for proper egg chamber formation. Knockdown of Cpsf5 or Cpsf6 attenuated border cell migration, increased both polar cells and outer border cells, and affected M-E transition at stage 6. Furthermore, knockdown of hrg, Clp and Cpsf160 showed similar phenotypes to knockdown of Cpsf5 or Cpsf6. Together, my data uncovered novel roles of brm, Cpsf5, Cpsf6 in follicle cells during Drosophila oogenesis.

中文摘要......................2
Abstract............................3 Introduction..........................4
Materials & Methods........8 Results...........................10 Discussion........................14 References........................16
Figures & Legends.......18
Tables.........................43

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