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研究生:王世賢
研究生(外文):WANG,SHIH-HSIEN
論文名稱:快篩技術結合影像處理軟體及手機APP執行三聚氰胺及農藥定量檢測
論文名稱(外文):Rapid screening technology combined with image processing softwares and smartphone APP for quantitative detection of melamine and pesticides
指導教授:王哲釧
指導教授(外文):WANG,Je-Chuang
口試委員:賴耀祥汪成斌胡國瑞吳國輝王哲釧
口試委員(外文):LAI,YAO XIANGWANG,CHENG BINHU,GUO RUIWu,Kuo-HuiWANG,Je-Chuang
口試日期:2020-05-14
學位類別:碩士
校院名稱:國防大學理工學院
系所名稱:材料科學與工程碩士班
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:82
中文關鍵詞:側流免疫層析法智慧型手機比色法快篩試片三聚氰胺加保扶
外文關鍵詞:lateral flow immunochromatographysmart phonecolorimetric methodrapid test stripmelaminecarbofuran
相關次數:
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  • 下載下載:6
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農產品中常有農藥殘留,超標的危害不論是急性、長期或慢性毒性對人體或環境都會造成很大的影響,常見的殘留超標農藥包括加保扶、芬普尼、佈飛松、達滅芬、毆殺滅、貝芬替等。另外不肖食品業者將三聚氰胺添加於奶粉、食物、飼料中,造成嚴重的食品安全事故。針對農藥或是三聚氰胺殘留檢測,傳統的化學分析法可達到實驗數據準確,但其過程耗時、高成本設備、需要專業背景人員操作;定性分析則相較簡單、快速、不需要專業背景,人人皆可使用,然無法達到定量的效果。
相較於傳統檢測模式(化學檢驗法及生化檢驗法),本研究開發了一種簡單操作、低成本、便於攜帶,智慧型手機化學分析APP之三聚氰胺及加保扶農藥即時檢測的比色傳感器、執行比色法並與相關軟體作比較。對三聚氰胺快篩試片以ImageJ軟體分析光密度檢測極限為0.40 ppm,而化學分析APP分析RGB值檢測極限為0.07 ppm;農藥快篩試片以加保扶為檢測樣品,以Photoshop軟體分析RGB值檢測極限為0.020 ppm,而化學分析APP分析RGB值檢測極限為0.018 ppm;其檢測極限均低於衛福部所訂定殘留容許量之標準,且手機化學分析APP具即時檢測及資訊傳輸處理之特性,可應用於食品安全、環境監測及國防安全等領域。

Pesticide residues are often found in agricultural products, and their toxicity, whether acute, long-term or chronic, has a great impact on both humans and the environment. Common pesticide residues often found to exceed the maximum residue levels (MRLs) include carbofuran, fipronil, profenofos, dimethomorph, oxamyl and carbendazim. In addition, unscrupulous food manufacturers have added melamine to milk powder, food, and animal feed in the past, causing serious food safety incidents. For the detection of pesticide or melamine residues, traditional chemical analysis can achieve accurate readings, but expensive equipment operated by well-trained personnel is required, and the process is time-consuming. Qualitative analysis, on the other hand, is simpler, quicker, and does not require professional personnel, being able to be used by anyone; however, it cannot be used for quantitative analysis.
In comparison with conventional detection methods (chemical and biochemical), we developed an easy-to-use, low-cost, portable chemical analysis device that runs using a smart phone App. This tool can perform colorimetric measurement to detect toxic substances in real time. In this study, we tested melamine and carbofuran as the target toxic agents, and used imaging software to determine the sensitivity of our device. For melamine, the rapid test strip analyzed by ImageJ was identified to have an optical density detection limit of 0.40 ppm, and the RGB value detection limit of the chemical analysis App was found to be 0.07 ppm. For carbofuran, Photoshop identified that the strip had an optical density detection limit of 0.020 ppm, and the RGB value detection limit of the App was found to be 0.018 ppm. These detection limits are all below the MRLs set by the Ministry of Health and Welfare of Taiwan, and the chemical analysis App can be used in the areas of food safety, environmental monitoring, and defense security.

謝辭 ii
摘要 iii
ABSTRACT iv
目錄 vi
表目錄 ix
圖目錄 x
1. 緒論 1
1.1 前言 1
1.2 研究動機及目的 1
1.3 研究方法 3
2. 文獻回顧 4
2.1 三聚氰胺及農藥殘留影響概述 4
2.2 三聚氰胺及農藥殘留檢測分析方法概述 5
2.3 比色法 6
2.3.1 比色法理論概述 6
2.3.2 比色法結合檢測技術之應用 8
2.3.3 固定光源及暗箱之應用 17
2.3.4 比色法結合智慧型手機檢測技術之應用 21
2.4 檢測極限概述 28
2.4.1 光密度分析檢測極限概述 28
2.4.2 比色法分析檢測極限概述 31
3. 實驗 35
3.1 實驗材料 35
3.2 實驗儀器與分析軟體 35
3.3 實驗步驟 36
3.3.1 手機拍照條件設定 36
3.3.2 不同環境光源下拍照 36
3.3.3 拍照環境設計 37
3.3.4 化學分析APP設計 37
3.3.5 三聚氰胺定量檢測 38
3.3.6 加保扶定量檢測 40
3.3.7 模擬樣品檢測 41
4. 結果與討論 43
4.1 不同環境光源下相對光密度分析 43
4.2 自製暗箱及光源優化分析 44
4.3 化學分析APP開發 47
4.4 三聚氰胺定量分析 52
4.5 加保扶定量分析 56
4.6 模擬樣品分析 61
5. 結論 64
參考文獻 66
自傳 70

參考文獻

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