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研究生:呂苡瑄
研究生(外文):LU,YI-HSUAN
論文名稱:蒲公英醇減緩巨噬細胞發炎反應
論文名稱(外文):Taraxeryl acetate attenuates inflammation of macrophages
指導教授:黃文忠黃文忠引用關係謝喜龍
指導教授(外文):HUANG,WEN-CHUNGHSIEH,HSI-LUNG
口試委員:吳淑如陳雅琳
口試委員(外文):WU,SHU-JUCHEN,YA-LING
口試日期:2020-07-02
學位類別:碩士
校院名稱:長庚科技大學
系所名稱:健康產業科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:82
中文關鍵詞:蒲公英醇發炎巨噬細胞細胞激素
外文關鍵詞:Taraxeryl acetateMacrophageInflammationCytokines
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碩士學位論文指導教授推薦書
碩士學位論文口試審定書
英文縮寫 i
中文摘要 iii
Abstract v
第一章緒論 1
第一節 前言 1
壹、活性氧自由基(Reactive Oxygen Species, ROS) 2
貳、脂質衍生物 3
參、細胞激素 ( cytokines )與趨化激素 ( chemokines ) 5
第二節 Toll-like receptor、脂多醣與Poly (I:C) 7
第三節 抗發炎藥物 10
第四節 巨噬細胞對發炎的影響 11
第五節 蒲公英醇 ( Taraxeryl acetate ) 12
第二章 假說與研究目的 14
第一節 假說 14
第二節 研究目的 15
第三章 材料與方法 16
第一節 細胞培養 16
壹、細胞株 16
貳、細胞繼代培養 16
第二節 樣品製備 18
壹、細胞中蛋白質萃取 18
貳、蛋白質定量 18
第三節 測定項目 19
壹、西方墨點法 ( Western Blot ) 19
貳、酵素免疫分析法 ( Enzyme-linked immunosorbent assay ;ELISA ) 22
參、免疫螢光染色法 ( Immunofluorescence ; IF ) 23
肆、 統計方法 24
第四章 實驗結果 25
第一節 TA影響LPS刺激RAW 264.7細胞之細胞激素及趨化激素分泌 25
第二節 TA對LPS刺激RAW 264.7細胞發炎媒介蛋白Stat3之影響 25
第三節 TA對LPS刺激RAW 264.7細胞發炎媒介蛋白之影響 26
第四節 TA對LPS刺激RAW 264.7細胞之發炎訊息表現 26
第五節 TA對Poly(I:C)刺激RAW 264.7細胞發炎細胞激素及趨化激素分泌之影響 28
第六節 TA對Poly(I:C)刺激RAW 264.7細胞發炎媒介蛋白之影響 29
第七節 TA對Poly(I:C)刺激RAW 264.7細胞發炎相關蛋白之影響 29
第五章 討論 31
第六章 結論 38
實驗結果圖 39
參考文獻 58
圖目錄
圖 1 TA處理LPS刺激RAW 264.7細胞之細胞激素與趨化激素表現 39
圖 2 TA處理LPS刺激RAW 264.7細胞之Stat3途徑蛋白表現 41
圖3  TA處理LPS刺激RAW 264.7細胞之發炎媒介蛋白表現 42
圖4  TA處理LPS刺激RAW 264.7細胞之MAPK途徑蛋白表現 43
圖5  TA處理LPS刺激RAW 264.7細胞之NF-kB途徑蛋白表現 44
圖6  TA處理LPS刺激RAW 264.7細胞之Immunofluorescence表現 45
圖7  TA處理LPS刺激RAW 264.7細胞之PI3K/Akt途徑蛋白表現 46
圖8  TA處理Poly(I:C)刺激RAW 264.7細胞之細胞激素與趨化激素表現 47
圖9  TA處理Poly(I:C)刺激RAW 264.7細胞之發炎媒介蛋白表現 48
圖10  TA處理Poly(I:C)刺激RAW 264.7細胞之之MAPK途徑蛋白表現 49
圖11  TA處理Poly(I:C)刺激RAW 264.7細胞之之MAPK途徑蛋白表現 50
附圖目錄
附圖 1為Taraxeryl acetate化學結構 51
附圖 2 為LPS誘導的MAPK及NF-kB的訊息傳導路徑 52
附圖 3 為Poly( I:C )誘導的NF-kB訊息傳導路徑 53
附圖 4 為Poly( I:C )誘導的MAPK訊息傳導路徑 54
附圖 5 為Toll-like receptor signaling 55
附圖 6 為TLR4信號通路示意圖 56
附圖 7 為TLR3信號通路示意圖 57


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