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研究生:黃郁辰
研究生(外文):Yu Chen Huang
論文名稱:探討GSL對B淋巴細胞之免疫調控活性
論文名稱(外文):Study of the immune-modulating activity of glycosphingolipids on B lymphocytes
指導教授:梅雅俊陳鈴津
指導教授(外文):Y. J. MeirL. J. Chen
學位類別:碩士
校院名稱:長庚大學
系所名稱:生物醫學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:57
中文關鍵詞:糖神經鞘脂質GD2腫瘤免疫B淋巴細胞免疫球蛋白類型轉換
外文關鍵詞:glycosphingolipidGD2tumor immunityB lymphocytesclass switch
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目錄
指導教授推薦書
口試委員會審定書
致謝………………………………………………………………………...…………iii
中文摘要………………………………………………………………………………iv
英文摘要………………………………………………………………………………v
目綠……………………………………………………………………………………vi
圖目錄……………………………………………………………..………………...viii
表目錄…………………………………………………………………………….…...ix
第一章 前言……………………………………………………………………….…1
第一節 癌症免疫……………………………………………………….…1
第二節 B淋巴細胞………………………………………………….….…2
第三節 Glycosphingolipids- GD2…………………………………………3
第四節 研究目的……………………………………………………….…6
第二章 材料與方法…………………………………………………………….……7
第一節 實驗動物…………………………………………………….……7
第二節 細胞培養…………………………………………………….……7
第三節 小鼠抗體…………………………………………………….……7
第四節 腫瘤浸潤淋巴細胞分離…………………………………….……8
第五節 B淋巴細胞之純化與培養…………………………….…….……9
第六節 GD2-Cer …………………………………………………….…..10
第七節 酵素連結免疫吸附分析法………………………………….…..11
第八節 流式細胞技術……………………………………………….…..12
第九節 即時聚合酶鏈鎖反應……………………………………….…..12
第十節 統計分析…………………………………………………….…..13

第三章 實驗結果………………………………………………………..….………14
第四章 討論………………………………………….…………………………..…21
參考文獻………………………………………………….…………………..………27
圖…………………………………………………….…………………..……………33
表……………………………………………………….……………..………………46


圖目錄
圖一、自 EL4 細胞株萃取的 gangliosides。………………………….….33
圖二、EL4 細胞株的 gangliosides 和萃取後的 GD2-Cer 之質譜分析。..34
圖三、純化的 GD2-Cer 於不同溶劑中之溶解度。……………….……..35
圖四、EL4 與 TIL 之表現型。…………………………………………...36
圖五、小鼠模式下 GD2-Cer 可自腫瘤細胞釋放、黏附至腫瘤環境中不
同的免疫細胞。…………………………………………………...37
圖六、小鼠模式下 GD2-Cer 可自腫瘤細胞釋放、黏附至脾臟內不同的
免疫細胞。………………………………………………….........38
圖七、細胞實驗下 GD2-Cer 可自腫瘤細胞釋放、黏附至不同的免疫細
胞。………………………………………………….......................39
圖八、純化的 GD2-Cer 可以黏附至不同的免疫細胞。…………….…40
圖九、純化的 GD2-Cer 對 T 淋巴細胞之免疫調控。……………….…41
圖十、未活化與活化後的 B 淋巴細胞上 GD2 的表現。…………….…42
圖十一、純化的 GD2-Cer 對 B 淋巴細胞之免疫調控。…………….…43
圖十二、純化的 GD2-Cer 對 B 淋巴細胞分化與免疫球蛋白釋放之
影響。…………………………………………………………….…44
圖十三、純化的 GD2-Cer 對 B 淋巴細胞 mRNA 表現之影響。…….…45

表目錄
表一、本篇研究使用的引子序列。…………………………………….46
表二、健康 C57B6 小鼠血清中多種 IgG 亞型含量。………………….47
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