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研究生(外文):Fiorency Santoso
論文名稱(外文):Establish a Simple ImageJ-Based Method for Cardiovascular Function Assessment in Fish and Daphnids
指導教授(外文):Chung-Der Hsiao
外文關鍵詞:Zebrafishwater fleasImageJheart rateblood flow velocity
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Cardiovascular disease, one of the potential disease which have an impact on human health. It can cause blood flow disruption to the heart, heart muscle injury, heart rate irregularity, and even death. This are rapidly becoming a major threat to the world due to its mortality rate on humans. Drugs and compounds, sometimes have missing-target properties that produce undesired side effects which lead into lethal consequences. Cardiotoxicity is one of the most common issues. Therefore, assessment of the drugs and compounds in animal model is needed. Zebrafish, as a low-level vertebrate is the emerging animal model that has many advantages. In the initial stage, the zebrafish body is transparent and the heart and blood circulation system already fully developed, hence the observation can be done. Moreover, zebrafish shares the same action potential, molecular, and physiological mechanism of heart like human. It also has closed blood circulation system same as human, making it possible to use zebrafish as blood model for human disease. Water fleas, an invertebrate species are among the most favored animals for aquatic toxicity testing due to their short life span, can be easily cultivated, and sensitive to chemicals. It is also a major component of fish zooplankton diet and a key user of freshwater food chains. Water fleas also have transparent body and large hearts, which allows studying the effects of toxicants on their cardiovasular system. Moreover, they are the first crustacean to have their genome sequenced and their most genes are identical to humans. This allows water fleas to be used as a reference model to address issues which directly relevant to human health. Previous methods have been established to calculate the heart rate and blood flow velocity. However, expensive and complicated apparatus is one of the limitations for the other scientists to conduct the experiment. Complicated manual script and programing language is another difficulty. Moreover, the effect of drug treatments on other cardiovascular function parameters, such as stroke volume and cardiac output remains to be explored. Therefore, the specific aim of this thesis is to provide low cost and easy setup for cardiovascular function assessment. By adapting ImageJ as an open source platform and establishing some mathematic formulation, we have successfully established method to measure the cardiovascular function and applied this method to evaluate and quantify the potential adverse effect of chemicals in zebrafish embryos and water fleas.
摘要 I

PART I: Development of a Simple ImageJ-Based Method for Dynamic Blood Flow Tracking in Zebrafish Embryos and Its Application in Drug Toxicity Evaluation 1-21

PART II: Utilization of High-Speed Video Recording Setup to Perform Cardiovascular Function Assessment in Water Fleas 22-38


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Figure 11 38

Table 1 19
1.Anton H, Harlepp S, Ramspacher C, Wu D, Monduc F, Bhat S, et al. Pulse propagation by a capacitive mechanism drives embryonic blood flow. Development. 2013:dev. 096768.
2.Boselli F, Freund JB, Vermot J. Blood flow mechanics in cardiovascular development. Cellular molecular life sciences. 2015;72(13):2545-59.
3.Isogai S, Horiguchi M, Weinstein BM. The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. Developmental biology. 2001;230(2):278-301.
4.Berman J, Hsu K, Look AT. Zebrafish as a model organism for blood diseases. British journal of haematology. 2003;123(4):568-76.
5.Dooley K, Zon LI. Zebrafish: a model system for the study of human disease. Current opinion in genetics development. 2000;10(3):252-6.
6.McGrath P, Li C-Q. Zebrafish: a predictive model for assessing drug-induced toxicity. Drug discovery today. 2008;13(9-10):394-401.
7.Dong W, Teraoka H, Yamazaki K, Tsukiyama S, Imani S, Imagawa T, et al. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo: local circulation failure in the dorsal midbrain is associated with increased apoptosis. Toxicological Sciences. 2002;69(1):191-201.
8.Martins T, Diniz E, Félix LM, Antunes L. Evaluation of anaesthetic protocols for laboratory adult zebrafish (Danio rerio). PloS one. 2018;13(5):e0197846.
9.Huang W-C, Hsieh Y-S, Chen I-H, Wang C-H, Chang H-W, Yang C-C, et al. Combined use of MS-222 (tricaine) and isoflurane extends anesthesia time and minimizes cardiac rhythm side effects in adult zebrafish. Zebrafish. 2010;7(3):297-304.
10.Basnet RM, Zizioli D, Guarienti M, Finazzi D, Memo M. Methylxanthines induce structural and functional alterations of the cardiac system in zebrafish embryos. BMC Pharmacology and Toxicology. 2017;18(1):72.
11.Basnet R, Guarienti M, Memo M. Zebrafish embryo as an in vivo model for behavioral and pharmacological characterization of Methylxanthine drugs. International journal of molecular sciences. 2017;18(3):596.
12.Pan X, Yu H, Shi X, Korzh V, Wohland T. Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy. Journal of biomedical optics. 2007;12(1):014034.
13.Iftimia NV, Hammer DX, Ferguson RD, Mujat M, Vu D, Ferrante AA. Dual-beam Fourier domain optical Doppler tomography of zebrafish. Optics express. 2008;16(18):13624-36.
14.Zeng Y, Xu J, Li D, Li L, Wen Z, Qu JY. Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging. Optics letters. 2012;37(13):2490-2.
15.Malone MH, Sciaky N, Stalheim L, Hahn KM, Linney E, Johnson GL. Laser-scanning velocimetry: a confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae. BMC biotechnology. 2007;7(1):40.
16.Anton H, Harlepp S, Ramspacher C, Wu D, Monduc F, Bhat S, et al. Pulse propagation by a capacitive mechanism drives embryonic blood flow. Development. 2013;140(21):4426-34.
17.Avdesh A, Chen M, Martin-Iverson MT, Mondal A, Ong D, Rainey-Smith S, et al. Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction. Journal of visualized experiments: JoVE. 2012(69).
18.Karlsson J, von Hofsten J, Olsson P-E. Generating transparent zebrafish: a refined method to improve detection of gene expression during embryonic development. Marine Biotechnology. 2001;3(6):522-7.
19.Watkins SC, Maniar S, Mosher M, Roman BL, Tsang M, St Croix CM. High resolution imaging of vascular function in zebrafish. PloS one. 2012;7(8):e44018.
20.Sampurna BP, Audira G, Juniardi S, Lai Y-H, Hsiao C-DJI. A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos. Inventions. 2018;3(2):21.
21.Moore FB, Hosey M, Bagatto B. Cardiovascular system in larval zebrafish responds to developmental hypoxia in a family specific manner. Frontiers in zoology. 2006;3(1):4.
22.Jacob E, Drexel M, Schwerte T, Pelster B. The influence of hypoxia and of hypoxemia on the development of cardiac activity in zebrafish larvae. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2002.
23.Marr D, Hildreth E. Theory of edge detection. Proceedings of the Royal Society of London Series B Biological Sciences. 1980;207(1167):187-217.
24.Jamison RA, Fouras A, Bryson-Richardson RJ. Cardiac-phase filtering in intracardiac particle image velocimetry. Journal of biomedical optics. 2012;17(3):036007.
25.Sato Y. Dorsal aorta formation: separate origins, lateral‐to‐medial migration, and remodeling. Development, growth differentiation. 2013;55(1):113-29.
26.Collymore C, Tolwani A, Lieggi C, Rasmussen S. Efficacy and safety of 5 anesthetics in adult zebrafish (Danio rerio). Journal of the American Association for Laboratory Animal Science. 2014;53(2):198-203.
27.Matthews M, Varga ZM. Anesthesia and euthanasia in zebrafish. ILAR journal. 2012;53(2):192-204.
28.Lockwood N, Parker J, Wilson C, Frankel P. Optimal anesthetic regime for motionless three-dimensional image acquisition during longitudinal studies of adult nonpigmented zebrafish. Zebrafish. 2017;14(2):133-9.
29.Ramlochansingh C, Branoner F, Chagnaud BP, Straka H. Efficacy of tricaine methanesulfonate (MS-222) as an anesthetic agent for blocking sensory-motor responses in Xenopus laevis tadpoles. PloS one. 2014;9(7):e101606.
30.Cho G, Heath D. Comparison of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the physiology of juvenile chinook salmon Oncorhynchus tshawytscha (Walbaum). Aquaculture research. 2000;31(6):537-46.
31.Schwartz FJ. Use of MS 222 in anesthetizing and transporting the sand shrimp. The Progressive Fish-Culturist. 1966;28(4):232-4.
32.Polese G, Winlow W, Di Cosmo A. Dose-dependent effects of the clinical anesthetic isoflurane on Octopus vulgaris: a contribution to cephalopod welfare. Journal of aquatic animal health. 2014;26(4):285-94.
33.De Luca E, Zaccaria GM, Hadhoud M, Rizzo G, Ponzini R, Morbiducci U, et al. ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos. Scientific Reports. 2014;4:4898.
34.Huertas A, Medioni G. Detection of intensity changes with subpixel accuracy using Laplacian-Gaussian masks. IEEE Transactions on Pattern Analysis and Machine Intelligence. 1986(5):651-64.
35.Gore AV, Monzo K, Cha YR, Pan W, Weinstein BM. Vascular development in the zebrafish. Cold Spring Harbor perspectives in medicine. 2012:a006684.
36.Sun P, Zhang Y, Yu F, Parks E, Lyman A, Wu Q, et al. Micro-electrocardiograms to study post-ventricular amputation of zebrafish heart. Annals of biomedical engineering. 2009;37(5):890-901.
37.Attili S, Hughes SMJPo. Anaesthetic tricaine acts preferentially on neural voltage-gated sodium channels and fails to block directly evoked muscle contraction. 2014;9(8):e103751.
38.Yazaki Y, Mochizuki S. Cellular Function and Metabolism: Springer Science & Business Media; 2012.
39.Klabunde R. Cardiovascular physiology concepts: Lippincott Williams & Wilkins; 2011.
40.Schwerte T, Überbacher D, Pelster B. Non-invasive imaging of blood cell concentration and blood distribution in zebrafish Danio rerio incubated in hypoxic conditions in vivo. Journal of Experimental Biology. 2003;206(8):1299-307.
41.Shi X, Foo YH, Sudhaharan T, Chong S-W, Korzh V, Ahmed S, et al. Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy. Biophysical journal. 2009;97(2):678-86.
42.Shi X, Shin Teo L, Pan X, Chong SW, Kraut R, Korzh V, et al. Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy. Developmental Dynamics. 2009;238(12):3156-67.
43.Craig MP, Gilday SD, Dabiri D, Hove JR. An optimized method for delivering flow tracer particles to intravital fluid environments in the developing zebrafish. Zebrafish. 2012;9(3):108-19.
44.Fieramonti L, Foglia EA, Malavasi S, D''Andrea C, Valentini G, Cotelli F, et al. Quantitative measurement of blood velocity in zebrafish with optical vector field tomography. Journal of biophotonics. 2015;8(1-2):52-9.
45.Lee SJ, Choi W, Seo E, Yeom E. Association of early atherosclerosis with vascular wall shear stress in hypercholesterolemic zebrafish. PloS one. 2015;10(11):e0142945.
46.Chen T, Huang Y. Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo. Analytical chemistry. 2017;89(19):10120-3.
47.Moreman J, Takesono A, Trznadel M, Winter MJ, Perry A, Wood ME, et al. Estrogenic mechanisms and cardiac responses following early life exposure to bisphenol A (BPA) and its metabolite 4-methyl-2, 4-bis (p-hydroxyphenyl) pent-1-ene (MBP) in zebrafish. Environmental science & technology. 2018;52(11):6656-65.
48.Weijts B, Gutierrez E, Saikin SK, Ablooglu AJ, Traver D, Groisman A, et al. Blood flow-induced Notch activation and endothelial migration enable vascular remodeling in zebrafish embryos. Nature communications. 2018;9(1):5314.
49.Xing Q, Huynh V, Parolari TG, Maurer-Morelli CV, Peixoto N, Wei Q. Zebrafish larvae heartbeat detection from body deformation in low resolution and low frequency video. Medical & biological engineering & computing. 2018;56(12):2353-65.
50.Zickus V, Taylor JM. 3D+ time blood flow mapping using SPIM-microPIV in the developing zebrafish heart. Biomedical optics express. 2018;9(5):2418-35.
51.Benslimane FM, Yalcin HC. Adaptation of a Mice Doppler Echocardiography Platform to measure cardiac flow velocities for embryonic chicken and adult Zebrafish. Frontiers in Bioengineering and Biotechnology. 2019;7:96.
52.Offem BO, Ayotunde EO. Toxicity of lead to freshwater invertebrates (Water fleas; Daphnia magna and Cyclop sp) in fish ponds in a tropical floodplain. Water, air, and soil pollution. 2008;192(1-4):39-46.
53.Kundu A, Singh G. Dopamine synergizes with caffeine to increase the heart rate of Daphnia. F1000Research. 2018;7.
54.Chung W, Song JM, Lee J. The Evaluation of Titanium Dioxide Nanoparticle Effects on Cardiac and Swimming Performance of Daphnia magna. International Journal of Applied Environmental Sciences. 2016;11(6):1375-85.
55.Jardim GM, Armas EDd, Monteiro RTR. Ecotoxicological assessment of water and sediment of the Corumbataí River, SP, Brazil. Brazilian Journal of Biology. 2008;68(1):51-9.
56.Co-operation OfE, Development. Test No. 211: Daphnia magna reproduction test: OECD Publishing; 2012.
57.Guilhermino L, Diamantino T, Silva MC, Soares A. Acute toxicity test with Daphnia magna: an alternative to mammals in the prescreening of chemical toxicity? Ecotoxicology and Environmental Safety. 2000;46(3):357-62.
58.Rodgher S, Espíndola ELG, Lombardi AT. Suitability of Daphnia similis as an alternative organism in ecotoxicological tests: implications for metal toxicity. Ecotoxicology. 2010;19(6):1027-33.
59.Iwai CB, Somparn A, Noller B. Using zooplankton, Moina micrura Kurz to evaluate the ecotoxicology of pesticides used in paddy fields of Thailand. Pesticides in the Modern World—Risks and Benefits INTECH Open Access Publisher. 2011:267-80.
60.Greene M, Pitts W, Dewprashad B. Using Videography to Study the Effects of Stimulants on Daphnia magna. The American Biology Teacher. 2017;79(1):35-40.
61.Colbourne JK, Pfrender ME, Gilbert D, Thomas WK, Tucker A, Oakley TH, et al. The ecoresponsive genome of Daphnia pulex. Science. 2011;331(6017):555-61.
62.Zang C, Laia N, Gruse A, Comfort C, Felder M. Caffeine and acetylcholine decrease Daphnia magna heart rate. Journal of Undergraduate Biology Laboratory Investigations. 2019;2(2).
63.Fekete-Kertész I, Stirling T, Ullmann O, Farkas É, Kirchkeszner C, Feigl V, et al. How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test?─ Analyses of Factors Affecting the Sensitivity of the Test System. Periodica Polytechnica Chemical Engineering. 2018;62(3):257-64.
64.Kaas B, Krishnarao K, Marion E, Stuckey L, Kohn R. Effects of melatonin and ethanol on the heart rate of Daphnia magna. Impulse: the premier journal for undergraduate publications in the neurosciences. 2009.
65.Colmorgen M, Paul RJ. Imaging of physiological functions in transparent animals (Agonus cataphractus, Daphnia magna, Pholcus phalangioides) by video microscopy and digital image processing. Comparative Biochemistry and Physiology Part A: Physiology. 1995;111(4):583-95.
66.Downing E, Meddins R. A stroboscopic technique for measuring Daphnia heart-beat rate. Journal of Biological Education. 1983;17(3):257-9.
67.Paul RJ, Colmorgen M, Hüller S, Tyroller F, Zinkler D. Circulation and respiratory control in millimetre-sized animals (Daphnia magna, Folsomia candida) studied by optical methods. Journal of Comparative Physiology B. 1997;167(6):399-408.
68.Hoage T, Ding Y, Xu X. Quantifying cardiac functions in embryonic and adult zebrafish. Cardiovascular Development: Springer; 2012. p. 11-20.
69.Maceira AM, Prasad SK, Khan M, Pennell DJ. Reference right ventricular systolic and diastolic function normalized to age, gender and body surface area from steady-state free precession cardiovascular magnetic resonance. European heart journal. 2006;27(23):2879-88.
70.Bäumer C, Pirow R, Paul R. Circulatory oxygen transport in the water flea Daphnia magna. Journal of Comparative Physiology B. 2002;172(4):275-85.
71.Sampurna B, Audira G, Juniardi S, Lai Y-H, Hsiao C-D. A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos. Inventions. 2018;3(2):21.
72.Santoso F, Sampurna BP, Lai Y-H, Liang S-T, Hao E, Chen J-R, et al. Development of a Simple ImageJ-Based Method for Dynamic Blood Flow Tracking in Zebrafish Embryos and Its Application in Drug Toxicity Evaluation. Inventions. 2019;4(4):65.
73.GOWDA C. Don''t be puzzled by cardiovascular concepts. Nursing made Incredibly Easy. 2008;6(4):27-30.
74.Piskorski J, Guzik P. Filtering poincare plots. Computational methods in science and technology. 2005;11(1):39-48.
75.Khan Q, Khan M. Effect of temperature on waterflea Daphnia magna (Crustacea: Cladocera). Nature Precedings. 2008:1-.
76.Özdemir S, Altun S, Arslan H. Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.). Toxicology reports. 2018;5:125-33.
77.Bownik A, Pawłocik M, Sokołowska N. Effects of neonicotinoid insecticide acetamiprid on swimming velocity, heart rate and thoracic limb movement of Daphnia magna. Pol J Nat Sci. 2017;32:481-93.
78.Chorro FJ, Such-Belenguer L, López-Merino V. Animal models of cardiovascular disease. Revista Española de Cardiología (English Edition). 2009;62(1):69-84.
79.Thorp JH, Covich AP. Ecology and classification of North American freshwater invertebrates: Academic press; 2009.
80.Monahan‐Earley R, Dvorak AM, Aird WC. Evolutionary origins of the blood vascular system and endothelium. Journal of Thrombosis and Haemostasis. 2013;11:46-66.
81.Molnar C, Gair J. Concepts of Biology: 1st Canadian Edition. 2015.
82.Ebert D. Ecology, epidemiology, and evolution of parasitism in Daphnia: National Library of Medicine; 2005.
83.Colmorgen M, Paul RJJCB, Physiology PPA. Imaging of physiological functions in transparent animals (Agonus cataphractus, Daphnia magna, Pholcus phalangioides) by video microscopy and digital image processing. 1995;111(4):583-95.
84.Usanov DA, Skripal AV, Usanov AD, Skripal AV, Abramov AV, editors. Laser diagnostics of daphnia oscillations. Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II; 2001: International Society for Optics and Photonics.
85.Baylor EJTBB. Cardiac pharmacology of the cladoceran, Daphnia. 1942;83(2):165-72.
86.Otto CM. Textbook of Clinical Echocardiography E-Book: Elsevier Health Sciences; 2013.
87.Chaudhuri K, Selvaraj S, Pal A. Studies on the genotoxicity of endosulfan in bacterial systems. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 1999;439(1):63-7.
88.Matsuda K, Buckingham SD, Kleier D, Rauh JJ, Grauso M, Sattelle DB. Neonicotinoids: insecticides acting on insect nicotinic acetylcholine receptors. Trends in pharmacological sciences. 2001;22(11):573-80.
89.Tomizawa M, Casida JE. Neonicotinoid insecticide toxicology: mechanisms of selective action. Annu Rev Pharmacol Toxicol. 2005;45:247-68.
90.Tišler T, Jemec A, Mozetič B, Trebše P. Hazard identification of imidacloprid to aquatic environment. Chemosphere. 2009;76(7):907-14.
91.Ma X, Li H, Xiong J, Mehler WT, You J. Developmental toxicity of a neonicotinoid insecticide, acetamiprid to zebrafish embryos. Journal of agricultural and food chemistry. 2019;67(9):2429-36.
92.Jemec A, Tišler T, Drobne D, Sepčić K, Fournier D, Trebše P. Comparative toxicity of imidacloprid, of its commercial liquid formulation and of diazinon to a non-target arthropod, the microcrustacean Daphnia magna. Chemosphere. 2007;68(8):1408-18.
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