野生茶樹泛指生長於自然界，沒經過栽培馴化以及被大量利用的茶樹。目前在市場流通的野生茶分為兩種，分別為自然型野生茶和栽培型野生茶。本實驗室對於青心烏龍茶種進行研究，發現此茶種中含有特殊醯化黃酮醇配醣體結構化合物，經實驗證實此化合物可與飢餓素接受器結合，造成腸胃蠕動、促進生長激素分泌等類似飢餓素誘導機制，故命名為茶飢素。本研究主要針對高雄藤枝地區之野生茶種進行分析，除比較其成分與茶園茶之差異外，更希望能從中找尋活性類似或更勝茶飢素之化合物。在使用液相層析串聯質譜儀分析後，發現其化學結構斷裂片段類似茶飢素之化合物之槲皮素或山柰酚的訊號結構片段，共有TGL1、TGL2、 TGL3及TGL4四種，初步推測皆為類茶飢素化合物。之後再使用styrene-divinylbenzene (ST-DVB)及Sephadex LH-20進行管柱純化及高效能液相層析儀及薄層層析法進行回收，再以Sephadex LH-20管柱純化去除殘留酸，最後以核磁共振儀(NMR)鑑定結構。NMR進行結構鑑定後，發現其中一種類茶飢素化合物TGL3的結構中鼠李糖基團(rhamnoside)接在黃酮醇主結構B環4’位羥基上。此化合物根據本實驗室過去分子對接模擬(molecular docking)的結果，由於4''位上接了鼠李糖基團，會阻礙類茶激素化合物進入生長激素受體(growth hormone receptor),故推測應該無明顯的刺激生長激素分泌的活性。

Wild tea trees are defined as tea plant grows in natural environment without domestication and heavy use. Currently, wild tea found in the market can be classified into two types, natural growth and human cultivation. we found a unique acylated flavone tetraglycoside in the "ching-hsing" oolong tea that increases gastrointestinal emptying and causes hunger. These compounds can bind ghrelin receptors and trigger physiological responses through the same molecular mechanisms as ghrelin. Due to these similar responses, these compounds were named Teaghrelins. Teaghrelin has shown high potential in developing treatments for growth hormone deficiency. My research is to find teaghrelin analogs from wild tea in Taiwan. In this study, tea samples were collected from in Tengjhih area of Kaohsiung and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS / MS). Four compounds from wild tea sample, TGL1, TGL2, TGL3 and TGL4, had similar ion fragments to teaghrelin. It is speculated that all four compounds have quercetin or kaempferol structure.Purified with styrene-divinylbenzene (ST-DVB) and Sephadex LH-20. Recovery using high performance liquid chromatography and thin layer chromatography, purified by Sephadex LH-20 column to remove acid. Finally, the structure was identified by nuclear magnetic resonance (NMR). It was found that in the structure of the compound TGL3, the rhamnose group is bonded to the hydroxyl group 4 ''of the B ring of the main structure of flavonol. This allows TGL3 to bind to the hungry receptor less than TG-1 and TG-2. According to the results of molecular docking in our laboratory, because 4'' is connected to rhamnoside, it is speculated that there should be no significant activity to stimulate growth hormone secretion.

中文摘要 i
Abstract iii
目錄 v
圖目錄 viii
表目錄 ix
第一章　前言 1
第二章　文獻回顧 3
第一節　茶葉介紹 3
一、臺灣茶介紹 3
二、茶葉製作 6
三、茶葉的分類 8
四、茶葉的保健功效 9
第二節　野生茶介紹 11
一、野生茶介紹 11
二、中國野生茶介紹 13
三、臺灣野生茶介紹 15
第三節　茶飢素 17
一、飢餓素的發現 17
二、茶飢素的發現 17
三、茶飢素之相關研究 18
第三章　材料與方法 21
第一節　研究流程 21
第二節　實驗試劑與材料 22
一、茶葉樣品來源 22
二、試驗試劑與材料 23
三、儀器設備 23
第三節　實驗方法 25
一、液相層析樣品處理及方法 25
二、串聯質譜儀樣品處理與分析方法 26
三、萃取醯化黃酮醇配糖體化合物及核磁共振鑑定結構 27
第四節　試驗試劑與儀器設備 29
一、試驗試劑與材料 29
二、儀器設備 29
第四章　結果 31
第一節　野生茶樣HPLC分析結果 31
第二節　串聯質譜儀分析結果 33
第三節　萃取類茶飢素化合物與核磁共振儀分析化合物結構 34
第五章 討論 37
第六章 圖表 39
第七章 參考文獻 65

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