(3.239.33.139) 您好!臺灣時間:2021/03/02 16:33
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:張育奇
研究生(外文):Yu-Chi Chang
論文名稱:高雄市藤枝地區野生茶成份之分析
論文名稱(外文):Analysis of wild tea ingredients in Tengjhih area of Kaohsiung
指導教授:曾志正曾志正引用關係
口試委員:張德卿郭賓崇林南海陳瑛宜
口試日期:2020-01-20
學位類別:博士
校院名稱:國立中興大學
系所名稱:生物科技學研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:70
中文關鍵詞:茶飢素野生茶生長激素受體
外文關鍵詞:teaghrelinwild teagrowth hormone receptor
相關次數:
  • 被引用被引用:0
  • 點閱點閱:49
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
野生茶樹泛指生長於自然界,沒經過栽培馴化以及被大量利用的茶樹。目前在市場流通的野生茶分為兩種,分別為自然型野生茶和栽培型野生茶。本實驗室對於青心烏龍茶種進行研究,發現此茶種中含有特殊醯化黃酮醇配醣體結構化合物,經實驗證實此化合物可與飢餓素接受器結合,造成腸胃蠕動、促進生長激素分泌等類似飢餓素誘導機制,故命名為茶飢素。本研究主要針對高雄藤枝地區之野生茶種進行分析,除比較其成分與茶園茶之差異外,更希望能從中找尋活性類似或更勝茶飢素之化合物。在使用液相層析串聯質譜儀分析後,發現其化學結構斷裂片段類似茶飢素之化合物之槲皮素或山柰酚的訊號結構片段,共有TGL1、TGL2、 TGL3及TGL4四種,初步推測皆為類茶飢素化合物。之後再使用styrene-divinylbenzene (ST-DVB)及Sephadex LH-20進行管柱純化及高效能液相層析儀及薄層層析法進行回收,再以Sephadex LH-20管柱純化去除殘留酸,最後以核磁共振儀(NMR)鑑定結構。NMR進行結構鑑定後,發現其中一種類茶飢素化合物TGL3的結構中鼠李糖基團(rhamnoside)接在黃酮醇主結構B環4’位羥基上。此化合物根據本實驗室過去分子對接模擬(molecular docking)的結果,由於4''位上接了鼠李糖基團,會阻礙類茶激素化合物進入生長激素受體(growth hormone receptor),故推測應該無明顯的刺激生長激素分泌的活性。
Wild tea trees are defined as tea plant grows in natural environment without domestication and heavy use. Currently, wild tea found in the market can be classified into two types, natural growth and human cultivation. we found a unique acylated flavone tetraglycoside in the "ching-hsing" oolong tea that increases gastrointestinal emptying and causes hunger. These compounds can bind ghrelin receptors and trigger physiological responses through the same molecular mechanisms as ghrelin. Due to these similar responses, these compounds were named Teaghrelins. Teaghrelin has shown high potential in developing treatments for growth hormone deficiency. My research is to find teaghrelin analogs from wild tea in Taiwan. In this study, tea samples were collected from in Tengjhih area of Kaohsiung and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS / MS). Four compounds from wild tea sample, TGL1, TGL2, TGL3 and TGL4, had similar ion fragments to teaghrelin. It is speculated that all four compounds have quercetin or kaempferol structure.Purified with styrene-divinylbenzene (ST-DVB) and Sephadex LH-20. Recovery using high performance liquid chromatography and thin layer chromatography, purified by Sephadex LH-20 column to remove acid. Finally, the structure was identified by nuclear magnetic resonance (NMR). It was found that in the structure of the compound TGL3, the rhamnose group is bonded to the hydroxyl group 4 ''of the B ring of the main structure of flavonol. This allows TGL3 to bind to the hungry receptor less than TG-1 and TG-2. According to the results of molecular docking in our laboratory, because 4'' is connected to rhamnoside, it is speculated that there should be no significant activity to stimulate growth hormone secretion.
中文摘要 i
Abstract iii
目錄 v
圖目錄 viii
表目錄 ix
第一章 前言 1
第二章 文獻回顧 3
第一節 茶葉介紹 3
一、臺灣茶介紹 3
二、茶葉製作 6
三、茶葉的分類 8
四、茶葉的保健功效 9
第二節 野生茶介紹 11
一、野生茶介紹 11
二、中國野生茶介紹 13
三、臺灣野生茶介紹 15
第三節 茶飢素 17
一、飢餓素的發現 17
二、茶飢素的發現 17
三、茶飢素之相關研究 18
第三章 材料與方法 21
第一節 研究流程 21
第二節 實驗試劑與材料 22
一、茶葉樣品來源 22
二、試驗試劑與材料 23
三、儀器設備 23
第三節 實驗方法 25
一、液相層析樣品處理及方法 25
二、串聯質譜儀樣品處理與分析方法 26
三、萃取醯化黃酮醇配糖體化合物及核磁共振鑑定結構 27
第四節 試驗試劑與儀器設備 29
一、試驗試劑與材料 29
二、儀器設備 29
第四章 結果 31
第一節 野生茶樣HPLC分析結果 31
第二節 串聯質譜儀分析結果 33
第三節 萃取類茶飢素化合物與核磁共振儀分析化合物結構 34
第五章 討論 37
第六章 圖表 39
第七章 參考文獻 65
1.阮逸明, 臺灣之茶文化及其科學. 臺灣茶業研究彙報, 1997. 16: p. 79-85.
2.Liu, S.L., et al., Classifying the Variety, Production Area and Season of Taiwan Partially Fermented Tea by Near. Journal of Food and Drug Analysis, 2010. 18(1): p. 34-43
3.Tipoe, G.L., et al., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection. Cardiovascular & Haematological Disorders-Drug Targets, 2007. 7(2): p. 135-144.
4.Weinreb, O., et al., Neurological mechanisms of green tea polyphenols in Alzheimer''s and Parkinson''s diseases. The Journal of nutritional biochemistry, 2004. 15(9): p. 506-516.
5.Lo, Y.H., et al., Teaghrelins, unique acylated flavonoid tetraglycosides in Chin-shin oolong tea, are putative oral agonists of the ghrelin receptor. Journal of agricultural and food chemistry, 2014. 62(22): p. 5085-5091.
6.Hsieh, S.K., et al., Identification of biosynthetic intermediates of teaghrelins and teaghrelin-like compounds in oolong teas, and their molecular docking to the ghrelin receptor. Journal of Food and Drug Analysis, 2015. 23(4): p. 660-670.
7.李欣潔, 陳冠亨, & 曾志正. (2014). 烏龍茶種植海拔高度與其茶湯澀度的關聯性. 農林學報, 63(2), 107-113.
8.李台強 and 張清寬, 臺灣茶樹種原圖誌. 205pp. 茶業改良場編印, 2003.
9.蔡俊明, et al., 年度命名茶樹新品種臺茶 19 號及臺茶 20 號試驗報告. 臺灣茶業研究彙報, 2004. 23: p. 57-78.
10.Wang, M.M., et al., Tzen Oolong Tea Converted from Oolong Tea by Baking and Aging Periodically. 農林學報, 2014. 63(2): p. 83-90
11.Tian, J., et al., Bacterial and fungal communities in Pu''er tea samples of different ages. Journal of food science, 2013. 78(8): p. M1249-M1256.
12.Fraser, K., et al., Analysis of metabolic markers of tea origin by UHPLC and high resolution mass spectrometry. Food research international, 2013. 53(2): p. 827-835.
13.Mukhtar, H. and Ahmad, N. Tea polyphenols: prevention of cancer and optimizing health. The American journal of clinical nutrition, 2000. 71(6): p. 1698-1702.
14.Kuhnert, N., et al., Mass spectrometric characterization of black tea thearubigins leading to an oxidative cascade hypothesis for thearubigin formation. Rapid Communications in Mass Spectrometry, 2010. 24(23): p. 3387-3404.
15.折改梅, et al., 茶氨酸和没食子酸在普洱茶中的含量变化. 云南植物研究, 2005. 27(5): p. 572-576.
16.Oi, Y., et al., Antiobesity effects of Chinese black tea (Pu-erh tea) extract and gallic acid. phytotherapy research, 2012. 26(4): p. 475-481.
17.Zeng, L., et al., Effects of Pu-erh tea aqueous extract (PTAE) on blood lipid metabolism enzymes. Food & function, 2015. 6(6): p. 2008-2016.
18.Moore, R.J., et al., Green tea (Camellia sinensis) catechins and vascular function. British Journal of Nutrition, 2009. 102(12): p. 1790-1802.
19.Yang, Y.-C., et al., The protective effect of habitual tea consumption on hypertension. Archives of internal medicine, 2004. 164(14): p. 1534-1540.
20.Arab, L., et al., Green and black tea consumption and risk of stroke. Stroke, 2009. 40(5): p. 1786-1792.
21.Murase, T., et al., Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. International journal of obesity, 2002. 26(11): p. 1459.
22.Zheng, G., et al., Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo, 2004. 18(1): p. 55-62.
23.Juneja, L.R., et al., L-theanine—a unique amino acid of green tea and its relaxation effect in humans. Trends in Food Science & Technology, 1999. 10(6): p. 199-204.
24.Fung, T.T., et al., Dietary patterns, meat intake, and the risk of type 2 diabetes in women. Archives of internal medicine, 2004. 164(20): p. 2235-2240.
25.van Dam, R.M., et al., Dietary patterns and risk for type 2 diabetes mellitus in US men. Annals of internal medicine, 2002. 136(3): p. 201-209.
26.Hayat, K., et al., Tea and its consumption: benefits and risks. Critical reviews in food science and nutrition, 2015. 55(7): p. 939-954.
27.Abeywickrama, K., et al., Oral hypoglycaemic, antihyperglycaemic and antidiabetic activities of Sri Lankan Broken Orange Pekoe Fannings (BOPF) grade black tea (Camellia sinensis L.) in rats. Journal of ethnopharmacology, 2011. 135(2): p. 278-286.
28.Panagiotakos, D.B., et al., Long-term tea intake is associated with reduced prevalence of (type 2) diabetes mellitus among elderly people from Mediterranean islands: MEDIS epidemiological study. Yonsei medical journal, 2009. 50(1): p. 31-38.
29. Bowers, C., et al., Structure-activity relationships of a synthetic pentapeptide that specifically releases growth hormone in vitro. Endocrinology, 1980. 106(3): p. 663-667. 

30. Momany, F., et al., Design, synthesis, and biological activity of peptides which release growth hormone in vitro. Endocrinology, 1981. 108(1): p. 31-39. 

31. Smith, R.G., et al., Peptidomimetic regulation of growth hormone secretion. Endocrine reviews, 1997. 18(5): p. 621-645.
32. Howard, A.D., et al., A receptor in pituitary and hypothalamus that functions in growth hormone release. Science, 1996. 273(5277): p. 974. 

33. Kojima, M., et al., Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature, 1999. 402(6762): p. 656-660. 

34. Ariyasu, H., et al., Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans. The Journal of Clinical Endocrinology & Metabolism, 2001. 86(10): p. 4753-4758. 

35.Lo, Y.H., et al., Teaghrelins, unique acylated flavonoid tetraglycosides in Chin-shin oolong tea, are putative oral agonists of the ghrelin receptor. Journal of agricultural and food chemistry, 2014. 62(22): p. 5085-5091.
36.Hsieh, S.K., et al., Identification of biosynthetic intermediates of teaghrelins and teaghrelin-like compounds in oolong teas, and their molecular docking to the ghrelin receptor. Journal of Food and Drug Analysis, 2015. 23(4): p. 660-670.
37.Dou, J., et al., Rapid identification of acylated flavonol tetraglycosides in oolong teas using HPLC‐MSn. Phytochemical analysis, 2008. 19(3): p. 251-257.
38.Lee, V.S.-Y., et al., Structural determination and DPPH radical-scavenging activity of two acylated flavonoid tetraglycosides in oolong tea (Camellia
39. Yang, S., Liu, W., Lu, S., Tian, Y. Z., Wang, W. Y., Ling, T. J., and Liu, R. T., A Novel multifunctional compound camellikaempferoside B decreases Aβ production, interferes with Aβ aggregation, and prohibits Aβ-mediated neurotoxicity and neuroinflammation. ACS Chem. Neurosci., 7(4), 505-518 (2016).
40. Hsieh, S. K., Lo, Y. H., Wu, C. C., Chung, T. Y., & Tzen, J. T. (2015). Identification of biosynthetic intermediates of teaghrelins and teaghrelin-like compounds in oolong teas, and their molecular docking to the ghrelin receptor. journal of food and drug analysis, 23(4), 660-670.
41. Mong-Huai Su, Chih-Hua Tsou and Chang-Fu Hsieh 2007 Morphological Comparisons of Taiwan Native Wild Tea Plant (Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura) and Two Closely Related Taxa Using Numerical Methods Taiwania 52(1):70-83
42. 秦燕春, 王迎新, & 王美怡. (2012). 神秘野生茶: 北回归线上的照亮. 中华文化画报, (8), 64-71.
43. 吕宁, 周玉璠, & 冯廷佺. (2013). 福建现存野生茶树群落分布(Doctoral dissertation).
44. 杨学荣, 鲁国伟, 杨家利, 刘桂仙, & 张利军. (2012). 镇康县野生茶资源保护现状, 存在问题及对策. 云南农业科技, 1.
45. 马柱芳, & 谷芸. (2012). 德宏州野生茶资源调查与保护. 云南农业科技, 1.
46. 郭彤, 王湘平, & 詹梓金. (1997). 福建野生茶的生化成分及其利用前景. 福建茶叶, 1, 54-56.
47. 刘玉芳, 林朝赐, 秦春玲, & 杨春. (2011). 浅谈广西野生茶资源的利用与保护 (Doctoral dissertation).
48. 朱秀眉, 叶清超, & 詹梓金. (2000). 平和野生茶的调查及其利用意见. 福建茶叶, 3, 12-13.
49. 李慧, 宋维希, 周萌, 李友勇, 马玲, 段志芬, ... & 刘本英. (2013). 云南省野生茶树资源的鉴定评价与筛选. 湖南农业科学, (10), 4.
50. 周鍾瑄,《諸羅縣志》。南投:臺灣省文獻委員會,民國八十二年,頁一九四。
51. 黃叔璥,《台海使槎錄》卷三〈赤崁筆談〉。南投:臺灣省文獻委員會,民國八十 年,頁六二。
52. Kuo, P. C., Li, Y. C., Wu, R. H., & Tzen, J. T. (2019). Characterization of teaghrelin-like compounds from tea cultivars. Natural product research, 1-6.
電子全文 電子全文(網際網路公開日期:20230204)
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
無相關期刊
 
無相關點閱論文
 
系統版面圖檔 系統版面圖檔