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研究生:黃郁涵
研究生(外文):Yu-Han Huang
論文名稱:腸膜明串珠菌(Leuconostoc mesenteroides )TBE-8作為蜜蜂腸道益生菌之特性探討與開發應用
論文名稱(外文):Development and application of Leuconostoc mesenteroides TBE-8 as a probiotics for honey bee, Apis mellifera
指導教授:吳明城吳明城引用關係
指導教授(外文):Ming-Cheng Wu
口試委員:乃育昕林春福
口試日期:2020-07-24
學位類別:碩士
校院名稱:國立中興大學
系所名稱:昆蟲學系所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:119
中文關鍵詞:西方蜂蜂群崩解症候群腸道益生菌腸膜明串珠菌蜜蜂營養蜜蜂免疫
外文關鍵詞:Apis melliferaCCDprobioticLeuconostoc mesenteroideshoney bee nutritionhoney bee immunity
相關次數:
  • 被引用被引用:0
  • 點閱點閱:19
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目 錄
中文摘要 i
ABSTRACT ii
目 錄 iv
圖 目 錄 vi
表 目 錄 vii
附 錄 之 圖 目 錄 viii
附 錄 之 表 目 錄 ix
前 言 1
前人研究 4
1. 蜜蜂的重要性及蜜蜂異常的消失 4
2. 蜜蜂腸道微生物 13
3. 營養基因及免疫基因在蜜蜂生理上的功能 15
4. 益生菌 ( Probiotics ) 16
5. 乳酸菌 ( Lactic acid bacteria, LAB ) 18
6. 明串珠菌屬 (Leuconostoc spp.) 21
材料與方法 25
1. 供試蟲種及室內飼養 25
2. 供試菌種 25
3. 菌株TBE-8之菌種鑑定 25
4. 菌株TBE-8的生長特性 29
5. 菌株TBE-8對蜜蜂的影響 31
6. TBE-8菌液及醋酸抑制幼蟲芽孢桿菌之體外試驗 36
7. 數據統計分析 37
結 果 38
1. 菌株TBE-8與其他乳酸菌株親緣關係分析 38
2. 菌株TBE-8之定量平台 39
3. 菌株TBE-8之蜜蜂毒理測試 40
4. 菌株TBE-8之生長特性 40
5. 菌株TBE-8之蔗糖溶液耐受性及pH耐受性測定 42
6. 菌株TBE-8之發酵產物測定 43
7. 菌株TBE-8在蜜蜂腸道中之滯留 44
8. 蜂場蜂群內工蜂腸道之腸膜明串珠菌菌量分佈 44
9. 菌株TBE-8對蜜蜂生理之影響 45
10. 菌株TBE-8抑制幼蟲芽孢桿菌之體外試驗 47
11. 菌株TBE-8之量產開發 47
討 論 48
1. 菌株TBE-8與其他乳酸菌株親緣關係與特徵比較 48
2. 菌株TBE-8之螢光探針(TaqMan○R probe) 設計 49
3. 菌株TBE-8之蜜蜂毒理測試 50
4. 菌株TBE-8之生長特性 50
5. 菌株TBE-8之蔗糖溶液耐受性及pH耐受性測定 51
6. 菌株TBE-8之發酵產物測定 52
7. 菌株TBE-8及腸膜明串珠菌在蜜蜂腸道中之滯留 53
8. 菌株TBE-8對蜜蜂生理之影響 54
9. 菌株TBE-8抑制幼蟲芽孢桿菌之體外試驗 56
結 論 58
參 考 文 獻 59
圖 表 80
附 錄 圖 表 99
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