本篇論文主要是利用微波輔助合成的方式合成不同的聚合物點，由聚合物主體聚乙烯亞胺(PEI)與三種不同的分子結合而成的聚合物點，對其做最適化並比較三種聚合物點的螢光強度及其量子產率，並從中選出螢光強度、量子產率表現最優異的聚合物點，透過EDC/NHS Coupling reaction使磁性及螢光奈米顆粒結合，探討不同pH值反應下磁螢奈米顆粒的螢光強度。

This thesis pertains to the synthesis of various polymer dots (PDs) via microwave-assisted technique. In this study, three different polymer dots are generated by combining PEI--major component of polymer dots--with three different molecules. Then the three different PDs are optimized and compared in order to identify which PDs have the best performance regarding their fluorescence intensity and quantum yield. Finally, the polymer dots with the best fluorescence intensity and quantum yield are combined with magnetic nanoparticles via EDC/NHS coupling reaction in order to examine the fluorescence intensity under different pH values.

目次
謝誌 i
摘要 ii
Abstract iii
目次 iv
表目次 vii
圖目次 viii
第一章 緒論 1
1.1 前言 1
1.2 研究動機 2
第二章 研究背景 3
2.1 碳點簡介 3
2.2 聚合物點 6
2.2.1 聚合物點介紹 6
2.2.2 共軛型聚合物點 7
2.2.3 非共軛型聚合物點 8
2.3 微波輔助合成 9
第三章 合成各種聚合物點 11
3.1 實驗藥品與器材 11
3.1.1 實驗藥品 11
3.1.2 儀器器材 13
3.2 實驗步驟 14
3.2.1 微波輔助製備Glycerol-PEI PDs 14
3.2.2 微波輔助製備Glutathione-PEI PDs 14
3.2.3 微波輔助製備Citric acid-PEI PDs 15
第四章 實驗結果與討論 16
4.1 最適化研究 16
4.1.1 Glycerol-PEI PDs 16
4.1.2 Glutathione-PEI PDs 19
4.1.3 Citric acid-PEI PDs 22
4.2 表面分析 25
4.2.1 Glycerol-PEI PDs 25
4.2.2 Glutathione-PEI PDs 27
4.2.3 Citric acid-PEI PDs 29
4.3 量子產率 31
4.3.1 量子產率公式 31
4.3.2 量子產率計算 32
4.3.3 量子產率比較 35
第五章 磁性螢光奈米顆粒 36
5.1 磁性奈米顆粒 36
5.1.1 磁性奈米顆粒的合成 37
5.1.2 表面分析 38
5.2 磁螢奈米顆粒 42
5.2.1 磁螢奈米顆粒的合成 43
5.2.1 磁螢奈米顆粒的光學特性 44
5.2.2 表面分析 45
5.2.3 最適化研究 48
第六章 聚合物點離子探針 49
6.1 聚合物點鎘離子探針 49
6.1.1 聚合物點陰陽離子選擇性 50
6.1.2 聚合物點陰陽離子干擾測試 51
6.1.3 聚合物點對Cd2+離子偵測 52
第七章 未來展望與結論 53
參考文獻 54

表目次
表 4. 1 Glycerol-PEI 聚合物點之官能基表 26
表 4. 2 Glutathione-PEI 聚合物點之官能基表 28
表 4. 3 Citric acid-PEI 聚合物點之官能基表 30
表 4. 4 Glycerol-PEI PDs 計算量子產率之數據 32
表 4. 5 Glutathione-PEI PDs 計算量子產率之數據 33
表 4. 6 Citric acid-PEI PDs 計算量子產率之數據 34
表 5. 1 二氧化矽包覆氧化鐵(Iron oxide@SiO2)之官能基表 39
表 5. 2 磁螢奈米顆粒(Iron oxide@SiO2-NH2@PDs)之官能基表 47

圖目次
圖 1. 1 碳點的歷史 3
圖 1. 2 Top-down method 和Botton-up method 的示意圖 4
圖 2. 1 聚合物點(Polymer dots)示意圖 6
圖 2. 2 微波輔助合成與傳統水熱法差異圖 10
圖 4. 1 不同反應溫度合成 Glycerol-PEI 之螢光強度長條圖 16
圖 4. 2 不同反應時間合成 Glycerol-PEI 之螢光強度長條圖 17
圖 4. 3 不同莫耳數的甘油合成 Glycerol-PEI 之螢光強度長條圖 18
圖 4. 4 不同反應溫度合成 Glutathione-PEI 之螢光強度長條圖 19
圖 4. 5 不同反應時間合成 Glutathione-PEI 之螢光強度長條圖 20
圖 4. 6 不同莫耳數的穀胱甘肽合成 Glutathione-PEI 之螢光強度長條圖 21
圖 4. 7 不同反應溫度合成 Citric acid-PEI 之螢光強度長條圖 22
圖 4. 8 不同反應時間合成 Citric acid-PEI 之螢光強度長條圖 23
圖 4. 9 不同莫耳數的檸檬酸合成 Citric acid-PEI 之螢光強度長條圖 24
圖 4. 10 Glycerol-PEI 之FT-IR圖 25
圖 4. 11 Glutathione-PEI 之FT-IR圖 27
圖 4. 12 Citric acid-PEI 之FT-IR圖 29
圖 4. 13 Glycerol-PEI 之UV-VIS圖 32
圖 4. 14 Glutathione-PEI 之UV-VIS圖 33
圖 4. 15 Citric acid-PEI 之UV-VIS圖 34
圖 5. 1 氧化鐵及二氧化矽包覆氧化鐵之FT-IR圖 38
圖 5. 2 氧化鐵顆粒 (Iron oxide) 之TEM 圖 40
圖 5. 3 氧化鐵顆粒 (Iron oxide) 粒徑分布長條圖 40
圖 5. 4 二氧化矽包覆氧化鐵顆粒 (Iron oxide@SiO2) 之TEM 圖 41
圖 5. 5 二氧化矽包覆氧化鐵顆粒 (Iron oxide@SiO2) 粒徑分布長條圖 41
圖 5. 6 Fe3O4@SiO2-NH2@PDs 之UV-VIS 圖 44
圖 5. 7 磁螢奈米顆粒(Iron oxide@SiO2-NH2@PDs) 之FT-IR圖 46
圖 5. 8 在不同的pH值反應條件下磁螢奈米顆粒之螢光強度長條圖 48
圖 6. 1 聚合物點對陽離子選擇性測試 50
圖 6. 2 聚合物點對陰離子選擇性測試 50
圖 6. 3 陽離子干擾測試 51
圖 6. 4 陰離子干擾測試 51
圖 6. 5 聚合物點對不同濃度的Cd2+做偵測 52
圖 6. 6 聚合物點對不同濃度的Cd2+偵測線性關係圖 52

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