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研究生:蘇益正
研究生(外文):Su, Yi-Cheng
論文名稱:掃描式高光譜顯微影像系統之研發
論文名稱(外文):Development of the scanning hyperspectral microscope imaging sysyem
指導教授:陳永富陳永富引用關係
指導教授(外文):Chen, Yung-Fu
口試委員:蘇冠暐梁興弛余彥廷
口試委員(外文):Su, Kuan-WeiLiang, Hsin-ChihYu, Yan-Ting
學位類別:碩士
校院名稱:國立交通大學
系所名稱:電子物理系所
學門:自然科學學門
學類:物理學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:62
中文關鍵詞:高光譜顯微影像
外文關鍵詞:HyperspectralMicroscopic image
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本論文之目的為透過更改高光譜顯微掃描系統之架構,使其可以應用於更多層面。首先,針對高光譜顯微系統分別針對兩種不同掃描模式分別為步進式與連續式,並透過兩種不同光源重複實驗證明,其兩種不同掃描模式擁有相同之垂直與水平空間解析度。藉由掃描具空間解析反射率之樣品,得知在小範圍下連續式之掃描速度較步進式提升了近4倍之掃描速度與獲得更加連續的掃描成像。而後為了將高光譜系統應用於穿透率與螢光之量測,因此將高光譜反射式架構變更使其成為高光譜穿透式架構,並且針對Nd:YVO4 a-cut、Nd:YVO4 c-cut與Nd:GdVO4三種不同樣品分別進行穿透率與螢光之掃描。從結果得知即便是同一科晶體其穿透率與螢光於不同位置大小亦不相同,並且於晶體中穿透率與螢光強度呈現反相關。
The purpose of this article is to change the structure of the hyperspectral microscanning system, which is applied to different measurements. First, the hyperspectral scanning system has step by step scanning and continuous scanning. Experiments with two different light sources prove that these two different scanning modes have the same axial and lateral resolutions . The Continuous scanning is four times faster than step scanning and can improve the continuity of scanned images in a small range . In order to apply the hyperspectral system to transmittance and fluorescence measurement, the reflection structure is changed to a transmission structure. The three samples Nd: YVO4 a-cut, Nd: YVO4 c-cut and Nd: GdVO4 were scanned for transmission and fluorescence. Even the same crystal has different transmittance and fluorescence intensity at different positions. The fluorescence intensity is higher at the position where the transmittance in the crystal is poor.
摘要 i
ABSTRACT ii
誌謝 iii
目錄 iv
圖目錄 vi

第一章 1
緒論
1.1 研究背景與動機 1
1.2 論文架構 2
第二章 3
高光譜影像顯微鏡之掃描模式簡介
2.1 高光譜影像技術與應用 3
2.2 自動化系統掃描影像模式之發展 5
第三章 13
高光譜反射式顯微鏡之掃描模式比較
3.1 反射式顯微鏡實驗架構與方法 13
3.2 步進與連續掃描模式之影像解析度比較 16
3.3 具空間解析反射率之樣品量測結果與討論 24
3.4 步進與連續掃描模式之掃描範圍最佳化 29

第四章 31
高光譜穿透式顯微鏡應用於穿透率之量測
4.1 穿透率掃描穿透式顯微鏡實驗架構與方法 31
4.2 Nd:YVO4之穿透光譜與高光譜穿透率影像分析 34
4.3 Nd:GdVO4之穿透光譜與高光譜穿透率影像分析 40
第五章 44
高光譜穿透式顯微鏡應用於螢光之量測
5.1 螢光掃描穿透式顯微鏡實驗架構與方法 44
5.2 Nd:YVO4之螢光光譜與高光譜螢光影像分析 49
5.3 Nd:GdVO4之螢光光譜與高光譜螢光影像分析 55
第六章 59
總結與未來展望
參考文獻 61
[1] A. F. H. Goetz, G. Vane, J. Solomon, and B. N. Rock, “Imaging Spectrometry for Earth Remote Sensing,” Science 228(4704), 1147-1153 (1985).
[2] S. Selci, “Phase and index of refraction imaging by hyperspectral reflectance confocal microscopy,” Molecules 21(12), 1727(2016).
[3] G. Bellisola, and M. Sorio, “Infrared spectroscopy and microscopy in cancer research and diagnosis,” Am. J. Cancer Res. 2(1), 1-21 (2012).
[4] D. C. Fernandez, R. Bhargava, S. M. Hewitt, and I. W. Levin, “Infrared spectroscopic imaging for histopathologic recognition,” Nat. Biotechnol. 23(4), 469-474 (2005).
[5] A. H. Colagar, M. J. Chaichi, and T. Khadjvand, “Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue,” Biosciences 36(4), 669-677 (2011).
[6] V. Farley, M. Chamberland, P. Lagueux, A. Vallie`res, A. Villemaire, and J. Giroux, “Chemical agent detection and identification with a hyperspectral imaging infrared sensor,” Proc. SPIE 6661(2007).
[7] Ravn, C., Skibsted, E., & Bro, R. “Near-infrared chemical imaging (NIR-CI) on pharmaceutical solid dosage forms—comparing common calibration approaches, ”Journal of Pharmaceutical and Biomedical Analysis, 48(3), 554-561 (2008).
[8] M. Booth, R. Juškaitis, and T. Wilson, “Spectral confocal reflection microscopy using a white light source,” J. Eur. Opt. Soc., 3 8026 –8026 (2008).
[9] R. W. Havener, C. J. Kim, L. Brown, J. W. Kevek, J. D. Sleppy, P. L. McEuen, and J. Park, “Hyperspectral Imaging of Structure and Composition in Atomically Thin Heterostructures,” Nano Lett. 13(8), 3942-3946(2013).
[10] J. Bini, J. Spain, K. Nehal, V. Hazelwood, C. DiMarzio, and M. Rajadhyaksha, “Confocal mosaicing microscopy of human skin ex vivo: spectral analysis for digital staining to simulate histology-like appearance,” J. Biomed. Opt. 16(7), 076008 (2011).
[11] Denstedt, M., Pukstad, B. S., Paluchowski, L. A., Hernandez-Palacios, J. E., & Randeberg, L. L. “Hyperspectral imaging as a diagnostic tool for chronic skin ulcers,” In Photonic Therapeutics and Diagnostics IX (Vol. 8565, p. 85650N). International Society for Optics and Photonics (2013, March).
[12] Al Kafi, M. A., Mwidu, U., & Moftah, B. “Continuous versus step-by-step scanning mode of a novel 3D scanner for CyberKnife measurements.” Applied Radiation and Isotopes, 105, 88-91 (2015).
[13] H. Grahn, and P. Geladi, “Techniques and Applications of Hyperspectral Image Analysis,” John Wiley & Sons, (2007).
[14] A. H. Colagar, M. J. Chaichi, and T. Khadjvand, “Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue,” Biosciences 36(4), 669-677 (2011).
[15] https://www.global-optosigma.com/en_jp/software/motorize/controller-manual_en.html
[16] A.J. Singh, S.K. Sharma, P.K. Mukhopadhyay, S.M. Oak, “Dual wavelength operation in diode-end-pumped hybrid vanadate laser,” Pramana J. Phys. 75, 929 (2010).
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