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研究生(外文):TSAI, CHUN-YI
論文名稱(外文):Effects of theaflavin-3,3\'-digallate on trapping of methylglyoxal and antiglycative activities in MDCK epithelial cells induced by advanced glycation end products
指導教授(外文):Lo, Chih-Yu
外文關鍵詞:black teaantiglycationtheaflavin-33'-digallateadvanced glycation end productsMadin-Darby canine kidney epithelial cells
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摘要 I
Abstract III
謝誌 V
目錄 VI
圖次 IX
表次 X
壹、前言 1
貳、文獻回顧 2
一、 糖化反應 2
1. 反應途徑 2
2. 進階糖化終產物的形成與來源 4
3. 進階糖化終產物的代謝與疾病 6
4. 抗糖化作用 8
二、 糖尿病 10
1. 糖尿病分類 10
2. 相關症狀 12
三、 茶 14
1. 茶葉製程 14
2. 茶種類 14
3. 主要成分 16
4. 生物活性 18
參、實驗目的 19
肆、實驗架構 20
伍、材料與方法 21
一、 材料 21
1. 樣品來源 21
2. 細胞株來源 21
3. 化學藥品、溶劑 21
三、 實驗方法 25
1. MGO 清除能力測定 25
2. AGEs 的製備 27
3. 螢光 AGEs 的測定 27
4. 細胞培養 27
5. AGEs、TF3 及 AG 樣品配製 28
6. MDCK 細胞株細胞存活率測定(MTT assay) 28
7. MDCK 細胞株活性氧分子含量測定 29
8. 西方墨點法(Western blot) 31
9. 統計分析 36
陸、結果與討論 37
一、 MGO 清除能力測定 37
二、 螢光 AGEs 的測定 40
三、 MDCK 細胞株細胞存活率測定(MTT assay) 42
四、 MDCK 細胞株活性氧分子含量測定(ROS) 44
五、 MDCK 細胞對糖化相關蛋白質表現量之影響 46
六、 MDCK 細胞對抗氧化相關蛋白質表現量之影響 49
七、 MDCK 細胞對發炎相關蛋白質表現量之影響 52
柒、結論 54
捌、參考文獻 55
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