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研究生:林佩蓉
研究生(外文):Lin, Pei-Long
論文名稱:臺灣芋細菌性軟腐病的病因探討、病原檢測和可能的防治方法之研究
論文名稱(外文):Etiology, detection and potential control measures of bacterial soft rot of taro in Taiwan.
指導教授:黃健瑞
指導教授(外文):Huang, Chien-Jui
學位類別:碩士
校院名稱:國立嘉義大學
系所名稱:植物醫學系
學門:農業科學學門
學類:植物保護學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:78
中文關鍵詞:芋細菌性軟腐病Dickeya fangzhongdaiPectobacterium sp.軟腐細菌病原檢測嘉賜銅
外文關鍵詞:Taro bacterial soft rot diseaseDickeya fangzhongdaiPectobacterium sp.pathogen detectionkasuran
相關次數:
  • 被引用被引用:1
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  • 收藏至我的研究室書目清單書目收藏:1
摘要.............................................i
Abstract........................................ii
誌謝...........................................iii
目錄............................................iv
1.前言...........................................1
2.前人研究.......................................2
2.1.芋..........................................2
2.2.軟腐細菌....................................4
2.3.芋軟腐細菌..................................7
3.材料與方法.....................................8
3.1.樣本採集、分離..............................8
3.2.細菌培養和保存..............................8
3.3.分離菌株對芋致病性測定...................... 9
3.4.病原細菌分子鑑定............................9
3.5.病原細菌形態特徵...........................10
3.6.軟腐細菌之生理生化特性分析..................10
3.7.芋軟腐細菌致病能力..........................11
3.8.芋軟腐細菌對不同植物的致病力.................11
3.9.分子檢測芋軟腐細菌..........................12
3.10.芋軟腐細菌在不同氧氣含量下對氮素源的利用.....13
3.11.芋軟腐細菌對藥劑敏感性檢測..................13
3.12.芋軟腐細菌對銅劑抗性程度....................14
4.結果...........................................15
4.1.芋軟腐病....................................15
4.2.芋細菌性軟腐病...............................15
4.3.芋軟腐細菌分子鑑定...........................15
4.4.芋軟腐細菌在不同培養基上之形態特徵............16
4.5.芋軟腐細菌生理生化特性分析...................16
4.6.不同種芋軟腐細菌致病能力.....................19
4.7.芋軟腐細菌對不同植物的致病力.................19
4.8.分子檢測芋軟腐細菌..........................19
4.9.芋軟腐細菌在不同氧氣含量下對氮素源的利用......21
4.10.芋軟腐細菌對藥劑敏感性檢測..................22
4.11.芋軟腐細菌對銅劑抗性程度....................22
5.討論...........................................23
5.1.芋細菌性軟腐病...............................23
5.2.芋軟腐細菌分子鑑定............................24
5.3.芋軟腐細菌在不同培養基上形態特徵...............25
5.4.芋軟腐細菌生理生化特性分析.....................26
5.5.不同種芋軟腐細菌致病能力.......................27
5.6. 芋軟腐細菌對不同植物的致病力..................28
5.7.分子檢測芋軟腐細菌............................29
5.8.芋軟腐細菌在不同氧氣含量下對氮素源的利用........29
5.9.芋軟腐細菌對藥劑敏感性檢測.....................30
5.10.芋軟腐細菌對銅劑抗性程度......................31
參考文獻...........................................32
表.................................................41
圖.................................................64
安寶貞。1995。台灣農家要覽農作篇(三)。葉瑩編。160。
林富雄。1998。高雄區農土蔬菜。高雄區農業改良場編印。
吳正宗。2005。 認識化學肥料。肥料特性及合理化施肥。台南區農業改良場技術專刊,94:24–63。
曹伽瑜。2020。由Neocosmospora 及 Fusarium 屬病原真菌引起的芋軟腐病與可能的防治技術之探討。國立嘉義大學碩士論文。
黃祥益、陳明昭、黃賢喜。2006。水芋栽培管理技術。行政院農業委員會高雄區農業改良場農技報導,78:2–15。
黃祥益。2010。避免水芋連作障礙之輪作模式。高雄區農業專訊,73:12–13。
楊秀珠。2012。芋頭之病蟲害發生與管理。行政院農業委員會動植物防疫檢局、行政院農業委員農業藥物毒物試驗所編印。
趙佳鴻、沈原民、劉興隆、白桂芳。2015。芋苗期病害之發生與防治技術研究。臺中區農業改良場特刊:106–110。
賴文龍, 郭雅紋, 陳玟瑾。2012。臺中地區水芋肥培管理技術。臺中區農業專訊,79:6–10。.
蔡正賢、吳添益。2017。苗栗低區水田栽培土壤特性與施肥管理。行政院農業委員會苗栗區農業改良場。
謝文陽。2006。細菌分類的濫觴和演變。科學發展,48:59–66。
Alcorn, S.M., and Orum, T.V. (1988) Rejection of the names Erwinia carnegieana Standring 1942 and Pectobacterium carnegieana (Standring 1942) Brenner, Steigerwalt, Miklos and Fanning 1973: Request for an Opinion. International Journal of Systematic and Evolutionary Microbiology, 38: 132–134.
Alcorn, S.M., Orum, T.V., Steigerwalt, A.G., Foster, J.L., Fogleman, J.C., and Brenner, D.J. (1991) Taxonomy and pathogenicity of Erwinia cacticida sp. nov. International Journal of Systematic Bacteriology, 41: 197–212.
Alič, Š., Van Gijsegem, F., Pédron, J., Ravnikar, M., and Dreo, T. (2018) Diversity within the novel Dickeya fangzhongdai sp., isolated from infected orchids, water and pears. Plant Pathology, 67: 1612–1620.
Alič, Š., Pédron, J., Dreo, T., and Van Gijsegem, F. (2019) Genomic characterisation of the new Dickeya fangzhongdai species regrouping plant pathogens and environmental isolates. BMC genomics,20: 34.
Baghaee-Ravari, S., Rahimian, H., Shams-Bakhsh, M., Lopez-Solanilla, E., Antúnez-Lamas, M., and Rodríguez-Palenzuela, P. (2011) Characterization of Pectobacterium species from Iran using biochemical and molecular methods. European Journal of Plant Pathology, 129: 413–425.
Bhat, R. (2017) Sustainability Challenges in the Agrofood Sector. Wiley Blackwell Publishers, West Sussex, UK.
Brady, C.L., Cleenwerck, I., Denman, S., Venter, S.N., Rodríguez-Palenzuela, P., Coutinho, T.A., and De Vos, P. (2012) Proposal to reclassify Brenneria quercina (Hildebrand and Schroth 1967) Hauben et al. 1999 into a new genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria , reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii. International journal of systematic and evolutionary microbiology, 62: 1592–1602.
Brenner, D.J., Steigerwalt, A.G., Miklos, G.V., and Fanning, G.R. (1973) Deoxyribonucleic acid relatedness among erwiniae and other Enterobacteriaceae: the soft-rot organisms (genus Pectobacterium Waldee). International Journal of Systematic and Evolutionary Microbiology, 23: 205-216.
Charkowski, A.O. (2018) The changing face of bacterial soft – rot disease. Annual Reviews of Phytopathology, 56: 269–288.
Chao Y.C., Feng C.T., Ho W.C. (2006) First report of Aglaonema bacterial blight caused by Erwinia chrysanthemi in Taiwan. Plant Disease, 10: 1358–1358.
Chen, L.R., Lin, P.R., and Huang, C.J. (2020) First report of Pectobacterium aroidearum causing soft rot disease of white calla lily in Taiwan. Plant Disease, 104: 563.
Chuang, M.F., Tzeng, K.C., and Hsu, S.T. (1989) Soft rot of radish caused by Erwinia carotovora subsp. carotovora and Erwinia chrysanthemi. Plant Protection Bulletin, 31: 358–365.
Cigna, J., Dewaegeneire, P., Beury, A., Gobert, V., & Faure, D. (2017) A gapA PCR-sequencing assay for identifying the Dickeya and Pectobacterium potato pathogens. Plant Disease, 101: 1278–1282.
Cuppels, D., and Kelman, A. (1974) Evaluation of selective media for isolation of soft-rot bacteria from soil and plant tissue. Phytopathology, 64: 468–475.
Czajkowski, R., Pe´rombelon, M.C.M. , van Veen, J.A., and van der Wolf, J.M. (2011) Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathology, 60: 999–1013.
Czajkowski, R., Pérombelon, M.C.M., Jafra, S., Lojkowska, E., Potrykus, M., van der Wolf, J.M., and Sledz, W. (2015) Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review. Annals of Applied Biology, 166: 18–38.
Darrasse A., Priou S., Kotoujansky A., and Bertheau Y. (1994) PCR and restriction fragment length polymorphism of a pel gene as a tool to identify Erwinia carotovora in relation to potato diseases. Applied and Environmental Microbiology, 60: 1437–1443.
Dauga, C. (2002) Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies. International Journal of Systematic and Evolutionary Microbiology, 52: 531–547.
Dees, M.W., Lysøe, E., Rossmann, S., Perminow, J., and Brurberg, M.B. (2017) Pectobacterium polaris sp. nov., isolated from potato (Solanum tuberosum). International journal of systematic and evolutionary microbiology, 67: 5222–5229.
Duarte, V., De Boer, S.H., Ward, L.J., de Oliverira, M.C. (2004) Characterization of atypical Erwinia carotovora strains causing blackleg of potato in Brazil. Journal of Applied Microbiology, 96: 535–545.
Eden, P.A., Schmidt, T.M., Blakemore, R.P., and Pace, N.R. (1991) Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA-specific DNA. International Journal of Systematic and Evolutionary Microbiology, 41: 324–325.
Fessehaie, A., De Boer, S.H., and Lévesque, C.A. (2002) Molecular characterization of DNA encoding 16S-23S rRNA intergenic spacer regions and 16S rRNA of pectolytic Erwinia species. Canadian Journal of Microbiology, 48: 387–398.
Frank, J.A., Reich, C.I., Sharma, S., Weisbaum, J.S., Wilson, B.A., and Olsen, G.J. (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Applied Environmental Microbiology, 74: 2461–2470.
Gardan, L., Gouy, C., Christen, R., and Samson, R. (2003) Elevation of three subspecies of Pectobacterium carotovorum to species level:Pectobacterium atroseptium sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp, nov. International Journal of Systematic and Evolutionary Microbiology, 53: 381–391.
Grenier, A.M., Duport, G., Pagès, S., Condemine, G., and Rahbé, Y. (2006) The phytopathogen Dickeya dadantii (Erwinia chrysanthemi 3937) is a pathogen of the pea aphid. Applied Environmental Microbiology, 72: 1956–1965.
Govaerts, R. (2014) World checklist of Araceae. Royal Botanic Gardens, Kew, London.
Hanben, L., Moore, E.R.B, Vauterin, L., Steenackers, M., Mergaert, J. Verdonck, L., and Swings, J. (1998) Phylogenetic position of phytopathogens within the Enterobacteriaceae. Systematic and applied microbiology, 21: 384–397.
Hélias, V., Hamon, P., Huchet, E., van der Wolf, J.M., and Andrivon, D. (2011) Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya. Plant Pathology, 61: 339–345.
Keer, J.T., and Birch, L. (2003) Molecular methods for the assessment of bacterial viability. Journal of Microbiological Methods, 53, 175–183.
Khayi, S., Cigna, J., Chong, T.M., Quetu-Laurent, A., Chan, K.G., Hélias, V., and Faure, D. (2016) Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. International Journal of Systematic and Evolutionary Microbiology, 66: 5379–5383.
Kim, H.S., Ma, B., Perna, N.T., and Charkowski, A.O. (2009). Phylogeny and virulence of naturally occurring type III secretion system-deficient Pectobacterium strains. Applied Environmental Microbiology, 75: 4539–4549.
Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and Evolution, 35: 1547–1549.
Lee, Y.A., and Yu, C.P. (2006) A differential medium for the isolation and rapid identification of a plant soft rot pathogen, Erwinia chrysanthemi. Journal of Microbiological Methods, 64: 200–206.
Liao, S.W., Chiu, K. Y., and Hou, H.H. (1979) A yellow strain of Erwinia carotovora var. carotovora. Memoirs of the College of Agriculture National Taiwan University. 19: 19–27.
Lim, T.K. (2015) Araceae. Modified stems, roots, bulbs edible medicinal and non medicinal plants (Ed. 9): 454-492.
Lin, Y.H., Lee, P.J., Shie, W.T., Chern, L.L., and Chao, Y.C. (2015) Pectobacterium chrysanthemi as the dominant causal agent of bacterial soft rot in Oncidium “Grower Ramsey”. European Journal of Plant Pathology, 142: 331-343.
Lin, C.Y., Huang, C.W., Yang, H.R., Tsai C.H., Hsu, S.L., and Ni, H.F. (2016) Rice Bacterial Foot Rot Disease Caused by Dickeya zeae in Taiwan. Journal of Taiwan Agricultural Research, 65: 207–217.
Ma, B., Hibbing, M.E., Kim, H.S., Reedy, R.M., Yedidia, I., Breuer, J., Glasner, J.D., Perna, N.T., Kelman, A., and Charkowski, A.O. (2007) Host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya. Phytopathology, 97: 1150–1163.
Mahamoud, A., Chevalier, J., Alibert-Franco, S., Kern, W. V., and Pagès, J. M. (2007) Antibiotic efflux pumps in Gram-negative bacteria: the inhibitor response strategy. Journal of Antimicrobial Chemotherapy, 59: 1223–1229.
Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., DOW, M., Verdier, V., Beer, S.V., Machado, M.A., Toth, I., Salmond, G., and Toth, I.A.N. (2012) Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13: 614–629.
Moretti, C., Fakhr, R., Cortese, C., De Vos, P., Cerri, M., Geagea, L. Cleenwerck, I., and Buonaurio, R. (2016) Pectobacterium aroidearum and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Lebanon. European Journal of Plant Pathology, 144: 205–211.
Nabhan, S., Wydra, K., Linde, M., and Debener, T. (2012) The use of two complementary DNA assays, AFLP and MLSA, for epidemic and phylogenetic studies of pectolytic enterobacterial strains with focus on the heterogeneous species Pectobacterium carotovorum. Plant Pathology, 61: 498–508.
Nabhan, S., De Boer, S.H., Maiss, E., and Wydra, K. (2013) Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants. International Journal of Systematic and Evolutionary Microbiology, 63: 2520–2525.
Ooka, J.J. (1990) Taro diseases. A Guide for Field Identification, 9: 51–59.
Ozturk, M., Aksoy, H. M., Potrykus, M., and Lojkowska, E. (2018) Genotypic and phenotypic variability of Pectobacterium strains causing blackleg and soft rot on potato in Turkey. European Journal of Plant Pathology, 152: 143–155.
Parkinson, N., DeVos, P., Pirhonen, M., and Elphinstone, J. (2014) Dickeya aquatica sp. nov., isolated from waterways. International Journal of Systematic and Evolutionary Microbiology, 64: 2264-2266.
Pritchard, L., Humphris, S., Saddler, G.S., Parkinson, N.M., Bertrand, V., Elphinstone, J.G., and Toth, I.K. (2012) Detection of phytopathogens ofthe genus Dickeya using a PCR primer prediction pipeline for draft bacterial genome sequences. Plant Pathology, 62: 587 - 596.
Reverchon, S., and Nasser, W. (2013) Dickeya ecology, environment sensing and regulation of virulence programme. Environmental Microbiology Reports, 5: 622 - 636.
Samson, R., Legendre, J.B., Christen, R., Fischer-Le Saux, M., Achouak, W., and Gardan, L. (2005) Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. International Journal of Systematic and Evolutionary Microbioloy, 55: 1415–14 27.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual (Ed. 2). Cold spring harbor laboratory press.
Schneider, K.L., Marrero, G., Alvarez, A.M., and Presting, G.G. (2011) Classification of plant associated bacteria using RIF, a computationally derived DNA marker. PLoS One, 6: e18496.
Segall, R.H. (1971) Selective medium for enumerating Erwinia species commonly found in vegetable packinghouse waters. Phytopathology, 61: 425–426.
Smid, E.J., Jansen, A.H., and Tuijn, C.J. (1993) Anaerobic nitrate respiration by Erwinia carotovora subsp. atroseptica during potato tuber invasion. Applied Environmental Microbiology, 59: 3648–3653.
Su, C.C., and Leu, L.S. (1992) Soft rot of Oncidium "Gower Ramsey" and Cymbidium sp. caused by Erwinia carotovora subsp. carotovora. Plant Pathology Bulletin, 1: 190–195.
Tian, Y., Zhao, Y., Yuan, X., Yi, J., Fan, J., Xu, Z., Hu, B., Boer, S.H., and Li, X. (2016) Dickeya fangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (Pyrus pyrifolia). International Journal of Systematic and Evolutionary Microbiology, 66: 2831–2835.
Toth, I.K., Bertheau, Y., Hyman, L.J., Laplaze, L., López, M.M., McNicol, J., Niepold, F., Persson, P., Salmond, G.P.C., Sletten, A., van der Wolf, J.M., and Pérombelon, M.C.M. (1999) Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subsp. atroseptica. Journal of Applied Microbiology, 87: 770–781.
Tsay, J.G., and Tung, B.K. (1993) The diagnosis and control of melon diseases. Taiwan Agriculture, 29: 12–20.
Tsai, W.A., Lin, P.R. and Huang, C.J. (2019) First report of Dickeya fangzhongdai causing soft rot disease of Welsh onion in Taiwan. Journal of Plant Pathology, 101: 797–798.
Tzeng, K.C., and Hsu S.T. (1981) Identification and characterization of soft-rotting Erwinia in Taiwan. Plant Protection Bulletin, 23: 77-85.
Van Der Merwe, J., Coutinho, T., Korsten, L., and Van Der Waals, J.E. (2010) Pectobacterium carotovorum subsp. brasiliensis causing blackleg on potatoes in South Africa. European Journal of Plant Pathology, 126: 175–185.
Van Der Wolf, J. M., Nijhuis, E. H., Kowalewska, M. J., Saddler, G. S., Parkinson, N., Elphinstone, J. G., Pritchard, L., Toth, I.K., Lojkowska, E., Potrykus, M., Waleron, M., Vos, P., Cleenwerck, I., Pirhonen, M., Garlant, L., lias, V.H., Pothier, J.F., Pfluger, V., Duffy, B., Tsror, L., and Manulis, S. (2014) Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). International Journal of Systematic and Evolutionary Microbiology, 64: 768–774.
Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J. (1991) 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703.
Wei, X.Y., Chiu, Y.H., Deng, W. L., and Chu, C.C. (2019) First report of Dickeya dadantii causing stem rot of poinsettia in Taiwan. Plant Disease, 103: 143.
Zhong, F.X., Fan, Q.R., and Loria, R. (2016) A re-evaluation of the taxonomy of phytopathogenic genera Dickeya and Pectobacterium using whole – genome sequencing data. Systematic and Applied Microbiology, 39: 252–259.
Zhang, J., Hu, J., Shen, H., Zhang, Y., Sun, D., Pu, X., Yang Q., Fan, Q., and Lin, B. (2018) Genomic analysis of the Phalaenopsis pathogen Dickeya sp. PA1, representing the emerging species Dickeya fangzhongdai. BMC Genomics, 19: 782.

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