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研究生:陳思叡
研究生(外文):Chen, Szu-Jui
論文名稱:快速微流體晶片整合光學與膠體電泳系統應用於錦鯉皰疹病毒檢測
論文名稱(外文):An integrated microfluidic loop-mediated isothermal amplification platform with optical and gel electrophoresis system for koi herpesvirus detection
指導教授:李佳言傅龍明
指導教授(外文):Lee, Chia-YenFu, Long-min
口試委員:林哲信王耀男劉展冏
口試委員(外文):Lin, Che-HsinWang, Yao-NanLiu, Chan-Chiung
口試日期:2020-07-17
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:材料工程研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:92
中文關鍵詞:錦鯉皰疹病毒微流體晶片恆溫環狀擴增膠體電泳
外文關鍵詞:Koi herpesvirus (KHV)microfluidic chipLoop-mediated isothermal amplification (LAMP)Gel electrophoresis
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  • 被引用被引用:0
  • 點閱點閱:108
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目錄
摘要 I
Abstract III
謝誌 IV
目錄 V
表目錄 VIII
圖目錄 IX
簡寫說明 XII
符號索引 XIV
第1章 緒論 1
1.1前言 1
1.2病毒 1
1.3微機電系統 2
1.4微流體技術 3
1.5微流體晶片 4
1.6研究目的 7
第2章 文獻回顧 8
2.1錦鯉皰疹病毒的簡介 8
2.1.1錦鯉皰疹病毒的特性 8
2.1.2錦鯉皰疹病毒的危害 9
2.1.3錦鯉皰疹病毒的檢測方式 10
2.2核醣核酸增幅法 11
2.2.1 DNA的介紹 11
2.2.2聚合酶連鎖反應 14
2.2.3環狀恆溫核酸增幅法 17
2.3膠體電泳 21
2.4生物晶片 24
2.4.1生物晶片歷史 24
2.4.2生物晶片基材 25
2.2.3晶片雷射加工 26
2.4.4晶片熱壓接合 30
2.5光學判讀系統 31
第3章 材料與實驗方法 34
3.1 材料與設備 34
3.1.1微流體晶片基材選擇 34
3.1.2實驗使用之試劑 34
3.1.3實驗使用之儀器 35
3.1.4實驗使用之引子序列 35
3.2實驗架構規劃 36
3.3實驗系統建立 37
3.3.1晶片設計與製程 37
3.3.2晶片檢測系統 39
3.3.2.1晶片檢測系統設計及原理 39
3.3.3膠體電泳製程 48
3.3.4膠體電泳系統 49
3.3.4.1膠體電泳系統設計及原理 49
3.3.5檢測機台製作 54
3.4實驗流程 59
3.4.1前處理純化實驗流程 59
3.4.2 PCR-LAMP實驗流程 59
3.4.3晶片檢測系統實驗流程 63
3.4.4膠體電泳檢測系統實驗流程 63
第4章 結果與討論 65
4.1應用於 LAMP機制之微流體晶片建置 65
4.1.1建置微流體晶片設計之選擇 65
4.1.1.1單槽三層側邊開孔微流體晶片設計 65
4.1.1.2單槽三層蛇型管道微流體晶片設計 66
4.1.1.3單槽三層上方開孔微流體晶片設計 67
4.2 PCR機制驗證整合式平台於 LAMP機制之可行性 68
4.3微流體晶片於 LAMP機制下不同反應溫度之影響 70
4.4微流體晶片於 LAMP機制下不同反應時間之探討 74
4.5 KHV於LAMP機制下之專一性探討 78
4.6微流體晶片整合系統於LAMP機制下之檢測極限探討 79
4.7膠體電泳整合式平台之可行性 81
第5章 結論與未來展望 83
5.1結論 83
5.2未來展望 84
參考文獻 85
作者簡介 92

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