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研究生:林鴻傑
研究生(外文):Lin, Hong-Jie
論文名稱:鹽類濃度及有機添加物對春石斛芽體增殖之影響
論文名稱(外文):Effect of salt strength and organic additives on shoot multiplication of nobile-type Dendrobium
指導教授:金石文
指導教授(外文):Chin, Shih-Wen
口試委員:陳福旗宋品慧
口試委員(外文):Chen, Fure-ChyiSong,Pin-Huei
口試日期:2020-06-23
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:75
中文關鍵詞:春石斛蛋白腖活性碳澱粉組織培養
外文關鍵詞:Dendrobium nobilePeptoneActive carbonStarchTissue culture
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春石斛蘭(Nobile-type Dendrobium)是新興蘭科作物之一,並在出口市場上具有經濟價值和外銷潛力。本實驗室以30 MS培養基(30 g/L蔗糖+0.4 mg/L BA + 0.1 mg/L NAA 之MS培養基)對多個春石斛品系進行繼代培養後,部分品系的小植株呈現矮小或黃褐化等情形。因此,本研究目的以30 MS培養基為基礎,挑選12個春石斛蘭品系之莖節培養並改良培養基配方;調整大量元素濃度、使用澱粉作為替代碳源、添加有機添加物和活性碳等,改善春石斛組織培養之培養基。結果顯示,大量元素鹽濃度調整試驗,Den. ‘9013’培養於四個處理組(全量、3/4、2/4、1/4)之30MS培養基中,3/4處理組以根系發育較其他處理組為佳,植株存活率高達90%以上;而另以四個品系609 (09129)、FL-009 (11281)、NPUD-27 (10061)、NPUD-66 (11322)於兩個處理組(全量、3/4)30 MS培養基中,不定芽數、株高、葉和根數均無差異,但FL-009 (11281)的不定芽產生於3/4處理組較為快速;替代碳源試驗,Den. ‘9013’於添加10 g/L馬鈴薯澱粉+15 g/L蔗糖之3/4 MS培養基中,根系發育較另外兩組(糯米粉、蓬萊米粉)為佳;並以馬鈴薯澱粉作為替代碳源,命名為PS培養基;另以上述四個品系於有無馬鈴薯澱粉(PS、30MS)之培養基中,四個品系的整體植株發育均無差異,但於PS培養基中根系較短小、培植體無黃褐化;上述五個品系於有無有機添加物和活性碳之培養基中,均於PPS+AC培養基(1 g/L Peptone 和1 g/L AC之PS培養基)中植株發育較為佳;並依此結果,另以七個品系於PPS+AC培養基中;其中以NPUD-20 (09074-910-14)、NPUD-9 (09001-14-3)、NPUD-2 NPUD-71 (11241)植株生長發育為最佳。
The Dendrobium(Nobile-type)is a new genus of orchids, and it has great potential to be economically significant and important in the export market. In our laboratory, Den. nobile strains were subcultured in 30 MS (MS medium with 30 g/L sucrose, 0.4 mg/L BA and 0.1 mg/L NAA ). Although this medium supported rapid growth, however, some strains showed signs of abnormal growth. Therefore, in this study, select 12 strains of Dendrobium's shoots were cultured and improved 30 MS medium recipe. Includes adjustment of macro salts strength, added starch as an alternative carbon source, as well as the addition of organic matter and activated carbon. The result in the adjustment of macro salts strength test showed that the Den. ‘9013’ cultivated in four treatments (Full, 3/4, 2/4, 1/4) of 30MS medium, 3/4 30 MS had a better root development than other treatments, and survival rate of explants reached up to 90%. Another four strains 609 (09129), FL-009 (11281), NPUD-27 (10061), NPUD-66 (11322) were cultivated in (Full, 3/4) 30 MS medium showed no significant difference in growth, with the exception of the bud regeneration of FL-009 (11281) was faster in the 3/4 treatment. In the alternative carbon source test, Den.‘9013’was cultivated in 3/4 30MS medium containing 15 g/L sucrose, and 10 g/L potato starch(PS medium), root development is better than the other two treatments (glutinous rice, japonica rice). Therefore, the use of potato starch can be an alternative carbon source. In addition, the above four strains cultivated in PS and 30 MS medium(with or without potato starch) showed no significant difference in growth, however, the explants’ roots were relatively shorter. Furthermore, there was no sign of browning in the PS medium. The above five strains were cultivated in four mediums (with or without organic matter and activated carbon, AC). The results showed that PPS+AC medium (PS medium with 1 g/L Peptone and 1 g/L AC) yielded the best growth development. Based on this result, another 7 strains were cultivated in PPS+AC medium for comparison. The growth development of NPUD-20 (09074-910-14), NPUD-9 (09001-14-3), and NPUD-2NPUD-71 (11241) were relatively better in this medium.
摘要 I
Summary II
謝誌 IV
目錄 V
圖表目錄 VIII
培養基代號 X
壹、前言 1
貳、前人研究 2
一、 石斛蘭屬之介紹 2
二、 春石斛蘭之介紹 2
(一)植株型態 2
(二)生育特性 3
(三)產業概況 3
(四)繁殖 4
三、 培養基 7
(一)氮源 7
(二)碳源 8
(三)維生素和氨基酸 9
(四)植物生長調節劑 9
(五)有機添加物 10
(六)其他物質 11
參、材料與方法 13
一、試驗材料 13
二、植株材料準備 13
(一)扦插消毒與高芽誘導 13
(二)高芽繼代 13
(三)莖節培植體 14
(四)培養基備製和培養容器 14
(五)培養室條件 14
二、不同培養基成分對春石斛品系之芽體增殖影響 14
(一) 大量元素鹽濃度調整之試驗 14
(二) 食用澱粉作為替代碳源之試驗 15
(三) 添加有機添加物之試驗 16
(四) 添加活性碳之試驗 17
四、 試驗設計和統計分析 18
肆、結果 20
一、大量元素鹽濃度調整之試驗 20
(一)春石斛‘9013’於不同大量元素鹽濃度之30 MS培養基中芽體增殖之影響 20
(二) 四個春石斛品系於兩種大量元素鹽濃度(4/4、3/4)之30 MS培養基中芽體增殖之比較 20
二、食用澱粉作為替代碳源之試驗 21
(一)春石斛‘9013’於不同馬鈴薯澱粉濃度之3/4 30 MS培養基中芽體增殖之影響 21
(二)春石斛‘9013’於不同澱粉於3/4 30MS培養基中芽體增殖之影響 22
(三)四個春石斛品系於有無馬鈴薯澱粉之培養基中芽體增殖之比較 22
三、添加有機添加物之試驗 23
(一)春石斛‘9013’於不同蛋白腖濃度之PS培養基中芽體增殖之影響 23
(二)五個春石斛品系於不同有機添加物之PS培養基中芽體增殖之影響 23
四、添加活性碳之試驗 25
(一)五個春石斛品系於有無添加活性碳之PPS培養基中芽體增殖之影響25
(二)七個春石斛品系於PPS+AC培養基中芽體增殖之影響 26
伍、討論 27
陸、結論 31
柒、參考文獻 64
捌、作者簡介 75

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