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研究生:張孝森
研究生(外文):Hsiao-Sen Chang
論文名稱:頸部脊髓挫傷後舌肌活性對於肺C纖維活化後之反應
論文名稱(外文):Modulation of the extrinsic tongue muscle activity in response to pulmonary C-fiber activation following cervical spinal cord contusion
指導教授:李昆澤李昆澤引用關係
指導教授(外文):Kun-Ze Lee
學位類別:碩士
校院名稱:國立中山大學
系所名稱:生物科學系研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:60
中文關鍵詞:舌骨舌肌辣椒素肺C纖維頦舌肌中段頸部脊髓挫傷
外文關鍵詞:pulmonary C-fibershyoglossuscapsaicingenioglossusmid-cervical spinal cord contusion
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頸部脊髓損傷不僅會導致嚴重的呼吸障礙,也可能會影響上呼吸道阻力進而改變呼吸功能。舌外肌在維持上呼吸道暢通時扮演著重要的角色,其中頦舌肌控制舌頭向前伸;舌骨舌肌則控制舌頭往後縮。因此,本研究的目的在探討舌外肌的呼吸活性是否會受到中段頸部脊髓 (C3~C4) 挫傷影響。經麻醉之自發性呼吸大鼠在損傷後急性期 (3天)、亞慢性期 (13.6 ± 1.3 天) 及慢性期 (55.9 ± 3.1 天),給予吸入霧化辣椒素 (25、100 μg/ml) 以刺激肺C纖維活化,並測量舌外肌肌電圖、食道壓力、血壓與心跳的反應。結果顯示,在損傷後三天,中段頸部脊髓挫傷動物的舌外肌前吸氣放電活性明顯高於偽損傷動物。在這個時期給予吸入霧化辣椒素會誘導偽損傷動物的舌外肌吸氣活性大幅下降,但此反應並未表現在損傷動物身上。值得注意的是,在損傷後慢性時期,高劑量的霧化辣椒素 (100 μg/ml) 會誘導損傷動物的頦舌肌前吸氣放電活性抑制作用更為嚴重。上述的結果表明,中段頸部脊髓挫傷可能導致舌下運動輸出 (Hypoglossal motor outputs) 的改變,此變化與肺C纖維的反射調控有關。另一方面,頸部脊髓挫傷後的急性期,舌外肌呼吸活性的代償性增強可能有助於維持上呼吸道暢通。但慢性期時,肺C纖維誘導的抑制舌肌吸氣反射增強則會導致上呼吸道堵塞的風險增加。這些結果顯示頸部脊髓損傷會伴隨著舌肌呼吸活性的改變以及肺C纖維活化後呼吸反射的變化,說明舌肌的呼吸調控在頸部脊髓挫傷後的呼吸功能改變中可能扮演極重要的角色。此論文推測調控舌肌活性可能有助於舒緩頸部脊髓損傷後呼吸功能障礙的狀況。
Mid-cervical spinal cord injury not only results in severe respiratory dysfunction but may also affect the airway resistance. Extrinsic tongue muscles (i.e., genioglossus and hyoglossus) play an important role in the maintenance of upper airway patency. Activation of the genioglossus can protrude the tongue; while hyoglossus is responsible for the retraction of the tongue. The purpose of this study was to investigate whether respiratory activity of the tongue muscle was influenced by cervical (C3~C4) spinal cord injury. Extrinsic tongue muscle electromyogram and esophageal pressure were measured in response to inhalation of capsaicin-induced (25 and 100 μg/ml) pulmonary C-fiber activation at the acute (three days), subchronic (13.6 ± 1.3 days) and chronic (55.9 ± 3.1 days) stages in anesthetized and spontaneously breathing adult rats. The results demonstrated that pre-inspiratory burst activity of the extrinsic tongue muscle during the baseline was significantly greater in mid-cervical spinal contused animals than sham animals at the acute injured stage. Pre-inspiratory and inspiratory activity were significantly decreased during capsaicin inhalation in sham animals; however, this inhibitory response of the tongue muscle was blunted in contused animals at the acute injured stage. Surprisingly, high dose of capsaicin-induced inhibition of pre-inspiratory genioglossus muscle activity was more severe in chronically contused animals. On the above basis, cervical spinal cord injury alters upper airway motor outputs and their reflex modulation induced by activation of pulmonary C-fibers. The compensatory increase in pre-inspiratory and inspiratory activity of the extrinsic tongue muscle may help to maintain upper airway patency at the acute injury stage. However, robust attenuation of genioglossus activity may increase a risk of upper airway obstruction at the chronic injured stage. We suspected that modulation of respiratory tongue muscle activity could be a potential therapeutic target to relieve the respiratory compromise following cervical spinal cord injury.
論文審定書 i
中文摘要 ii
英文摘要 iii
目錄 v
圖次 vii
表次 ix
縮寫表 x
1.1 呼吸系統的調控機制 1
1.2 頸部脊髓損傷對呼吸功能的影響 2
1.3 頸部脊髓損傷對舌肌的影響 3
1.4 目的 4
第二章、材料與方法 5
2.1 實驗動物 5
2.2 頸部脊髓挫傷手術 5
2.3 辣椒素配置 6
2.4 麻醉手術及測量 6
2.5實驗流程 7
2.6 組織學 7
2.7 統計及數據分析 8
第三章、結果 10
3.1 頸部脊髓損傷後呼吸參數及體重變化 10
3.2 頸部脊髓損傷對於舌肌活性的影響 10
3.3 霧化辣椒素與溶劑對於呼吸模式的影響 11
3.4 霧化辣椒素對於舌肌的影響 11
3.5 組織學 12
第四章、討論 14
4.1 方法討論 14
4.2 中段頸部脊髓挫傷對於舌肌的影響 14
4.3 中段頸部脊髓挫傷對於吸入性辣椒素引起之肺化學反射的影響 15
4.4 生理影響 15
參考文獻 17
1.Del Negro CA, Funk GD, Feldman JL. Breathing matters. Nature reviews Neuroscience. 2018;19(6):351-367.
2.Inoue T, Nakayama K, Ihara Y, et al. Coordinated control of the tongue during suckling-like activity and respiration. Journal of oral science. 2017;59(2):183-188.
3.Pitts T, Rose MJ, Mortensen AN, et al. Coordination of cough and swallow: a meta-behavioral response to aspiration. Respiratory physiology & neurobiology. 2013;189(3):543-551.
4.Bautista TG, Sun QJ, Pilowsky PM. The generation of pharyngeal phase of swallow and its coordination with breathing: interaction between the swallow and respiratory central pattern generators. Progress in brain research. 2014;212:253-275.
5.Oo T, Watt JW, Soni BM, Sett PK. Delayed diaphragm recovery in 12 patients after high cervical spinal cord injury. A retrospective review of the diaphragm status of 107 patients ventilated after acute spinal cord injury. Spinal cord. 1999;37(2):117-122.
6.Bezdudnaya T, Hormigo KM, Marchenko V, Lane MA. Spontaneous respiratory plasticity following unilateral high cervical spinal cord injury in behaving rats. Experimental neurology. 2018;305:56-65.
7.Warren PM, Campanaro C, Jacono FJ, Alilain WJ. Mid-cervical spinal cord contusion causes robust deficits in respiratory parameters and pattern variability. Experimental neurology. 2018;306:122-131.
8.Wen MH, Lee KZ. Diaphragm and Intercostal Muscle activity after mid-cervical spinal cord contusion in the rat. Journal of neurotrauma. 2018;35(3):533-547.
9.Lee KZ, Kuo HC. Vagal control of breathing pattern after midcervical contusion in rats. Journal of neurotrauma. 2017;34(3):734-745.
10.Alvarez-Argote S, Gransee HM, Mora JC, et al. The Impact of Midcervical contusion injury on diaphragm muscle function. Journal of neurotrauma. 2016;33(5):500-509.
11.Toh CC, Lee TS, Kiang AK. The pharmacological actions of capsaicin and analogues. British journal of pharmacology and chemotherapy. 1955;10(2):175-182.
12.Lee KZ, Xu KJ. The impact of cervical spinal cord contusion on the laryngeal resistance in the rat. Journal of neurotrauma. 2019;36(3):448-459.
13.Gilliam EE, Goldberg SJ. Contractile properties of the tongue muscles: effects of hypoglossal nerve and extracellular motoneuron stimulation in rat. Journal of neurophysiology. 1995;74(2):547-555.
14.Peever JH, Duffin J. Respiratory control of hypoglossal neurons during sleep and wakefulness. In: Binder MD, Hirokawa N, Windhorst U, eds. Encyclopedia of Neuroscience. 2009:3441-3446.
15.Saboisky JP, Butler JE, Fogel RB, et al. Tonic and phasic respiratory drives to human genioglossus motoneurons during breathing. Journal of neurophysiology. 2006;95(4):2213-2221.
16.Lee KZ, Sandhu MS, Dougherty BJ, Reier PJ, Fuller DD. Influence of vagal afferents on supraspinal and spinal respiratory activity following cervical spinal cord injury in rats. Journal of applied physiology (Bethesda, Md : 1985). 2010;109(2):377-387.
17.Sauerland EK, Harper RM. The human tongue during sleep: electromyographic activity of the genioglossus muscle. Experimental neurology. 1976;51(1):160-170.
18.Burns SP, Little JW, Hussey JD, Lyman P, Lakshminarayanan S. Sleep apnea syndrome in chronic spinal cord injury: associated factors and treatment. Archives of physical medicine and rehabilitation. 2000;81(10):1334-1339.
19.Jelev A, Sood S, Liu H, Nolan P, Horner RL. Microdialysis perfusion of 5-HT into hypoglossal motor nucleus differentially modulates genioglossus activity across natural sleep-wake states in rats. The Journal of physiology. 2001;532(Pt 2):467-481.
20.Fenik VB, Davies RO, Kubin L. REM sleep-like atonia of hypoglossal (XII) motoneurons is caused by loss of noradrenergic and serotonergic inputs. American journal of respiratory and critical care medicine. 2005;172(10):1322-1330.
21.Zhurakovskaya E, Paasonen J, Shatillo A, et al. Global functional connectivity differences between sleep-like states in urethane anesthetized rats measured by fMRI. PloS one. 2016;11(5):e0155343.
22.Powell GL, Rice A, Bennett-Cross SJ, Fregosi RF. Respiration-related discharge of hyoglossus muscle motor units in the rat. Journal of neurophysiology. 2014;111(2):361-368.
23.Lee KZ, Lu IJ, Ku LC, Lin JT, Hwang JC. Response of respiratory-related hypoglossal nerve activity to capsaicin-induced pulmonary C-fiber activation in rats. Journal of biomedical science. 2003;10(6 Pt 2):706-717.
24.John J, Bailey EF, Fregosi RF. Respiratory-related discharge of genioglossus muscle motor units. American journal of respiratory and critical care medicine. 2005;172(10):1331-1337.
25.Wasicko MJ, Giering RW, Knuth SL, Leiter JC. Hypoglossal and phrenic nerve responses to carotid baroreceptor stimulation. Journal of applied physiology (Bethesda, Md : 1985). 1993;75(3):1395-1403.
26.Edwards IJ, Lall VK, Paton JF, et al. Neck muscle afferents influence oromotor and cardiorespiratory brainstem neural circuits. Brain structure & function. 2015;220(3):1421-1436.
27.Shevtsova NA, Marchenko V, Bezdudnaya T. Modulation of respiratory system by limb muscle afferents in intact and injured spinal cord. Frontiers in neuroscience. 2019;13:289.
28.Lee KZ, Chang YS. Recovery of the pulmonary chemoreflex and functional role of bronchopulmonary C-fibers following chronic cervical spinal cord injury. Journal of applied physiology (Bethesda, Md : 1985). 2014;117(10):1188-1198.
29.Tsai IL, Lee KZ. Attenuation of the pulmonary chemoreflex following acute cervical spinal cord injury. Journal of applied physiology (Bethesda, Md : 1985). 2014;116(7):757-766.
30.Zwetsloot KA, John CS, Lawrence MM, Battista RA, Shanely RA. High-intensity interval training induces a modest systemic inflammatory response in active, young men. Journal of inflammation research. 2014;7:9-17.
31.Trazzi S, Fuchs C, Viggiano R, et al. HDAC4: a key factor underlying brain developmental alterations in CDKL5 disorder. Human molecular genetics. 2016;25(18):3887-3907.
32.Sivilia S, Mangano C, Beggiato S, et al. CDKL5 knockout leads to altered inhibitory transmission in the cerebellum of adult mice. Genes, brain, and behavior. 2016;15(5):491-502.
33.Berlowitz DJ, Brown DJ, Campbell DA, Pierce RJ. A longitudinal evaluation of sleep and breathing in the first year after cervical spinal cord injury. Archives of physical medicine and rehabilitation. 2005;86(6):1193-1199.
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