臺灣博碩士論文加值系統

摘要 I
Abstract II
目錄 III
名詞縮寫表 XI
壹、 緒論 1
貳、 文獻回顧 3
一、 糖尿病 3
1.1 糖尿病前期 (Prediabetes) 3
1.2 糖尿病之診斷標準 4
1.3 糖尿病之分類 4
二、男性生殖與不孕症 6
2.1 男性不孕症之定義 6
2.2 男性不孕症之診斷 6
2.3 男性不孕症之成因 6
2.4 男性生殖內分泌調控 8
三、糖尿病與男性生殖 9
3.1 糖尿病與氧化壓力 9
3.2 糖尿病與生殖內分泌 9
3.3 動物誘導糖尿病模式探討 10
3.4 糖尿病與藥物 Metformin 10
四、海馬 11
4.1 海馬之簡介 11
4.2 海馬之成分分析 11
4.3 海馬之傳統功效 12
4.4 海馬之生理活性 12
4.5 海馬水解物 13
參、實驗流程 14
一、海馬水解物之製備 14
二、海馬水解物腸胃道分解物之製備 15
三、海馬水解物腸胃道分解物之純化 16
四、細胞實驗 17
五、動物實驗 18
肆、實驗材料與方法 19
一、實驗材料 19
1.1 乾燥海馬 19
1.2 細胞株 19
1.3 實驗動物 19
1.4 動物飼料 20
1.5 藥品 21
1.6 儀器設備 25
二、海馬成分分析 27
2.1 水分含量測定 27
2.2 粗脂肪含量測定 27
2.3 粗蛋白含量測定 27
2.4 灰分含量測定 28
三、海馬水解物之製備 28
四、海馬水解物之消化道試驗 28
4.1 腸胃道酵素水解物製備 28
五、海馬水解物腸胃道分解物之分析 29
5.1 蛋白質含量 (Bradford protein assay) 29
5.2 水解率測定 (Degree of hydrolysis, DH) 29
5.3 SDS 聚丙烯醯胺膠體電泳 (SDS-PAGE electrophoresis) 30
六、海馬水解物腸胃道分解物之純化 31
6.1 離子管柱層析 31
七、細胞實驗 31
7.1 細胞培養 31
7.2 細胞存活率測定 (MTT assay) 32
7.3 一氧化氮釋放量測定 32
7.4 睪固酮含量測定 33
八、動物實驗 33
8.1 糖尿病大鼠之誘導與分組 33
8.2 實驗動物飼養 34
8.3 血糖及胰島素含量測定 35
8.4 肝臟及腎臟生化數值分析 35
8.5 氧化壓力分析 36
8.6 發炎反應分析 38
8.7 生殖系統分析 40
8.8 生殖內分泌蛋白質表現量分析 41
九、統計分析 42
伍、結果 43
一、海馬水解物分析及純化 43
1.1 海馬水解物分析 43
1.2 海馬水解物之純化分離 44
二、細胞實驗 44
2.1 LPS 誘導 RAW 264.7 細胞發炎模式 44
2.2 H2O2 誘導 LC-540 細胞氧化壓力模式 45
三、動物實驗 46
3.1 SH 對於糖尿病大鼠血糖及胰島素含量之影響 46
3.2 SH 對於糖尿病大鼠臟器重量百分比及功能之影響 46
3.3 SH 對於糖尿病大鼠體內氧化壓力之影響 47
3.4 SH 對於糖尿病大鼠體內發炎反應之影響 48
3.5 SH 對於糖尿病雄性大鼠生殖系統之影響 48
3.6 SH 對於糖尿病雄性大鼠生殖內分泌蛋白表現量之影響 49
陸、討論 51
柒、結論 55
捌、參考文獻 56
玖、圖表 64
壹拾、附錄 92

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