臺灣博碩士論文加值系統

表目錄 V
圖目錄 VI
謝誌 VIII
摘要 X
Abstract XI
壹、前言 1
貳、文獻回顧 2
一、粥狀動脈硬化 2
1.1粥狀動脈硬化之病理機制 2
1.2粥狀動脈硬化之發展過程 2
1.3粥狀動脈硬化與泡沫細胞 3
二、以褐藻為基質之發酵物特性 3
2.1發酵物成分變化 3
2.2發酵物抗氧化能力變化 4
三、以B. subtilis為菌種之發酵物特性 8
3.1成分變化 8
3.2發酵物抗氧化能力的變化 9
3.3以B. subtilis為菌種之發酵物對粥狀動脈硬化之影響 10
四、巨噬細胞轉化泡沫細胞及發炎反應之蛋白質體分析 13
4.1利用蛋白質體學分析ox-LDL誘導泡沫細胞形成之蛋白質變化 13
4.2利用蛋白質體學分析鼠尾草酸抑制LPS刺激巨噬細胞發炎之蛋白質變化 13
參、實驗架構 15
肆、實驗材料與方法 16
一、實驗材料 16
1.1 試藥及耗材 16
1.2 儀器設備 18
1.3 實驗菌株來源 19
1.4 菌株培養基 19
1.5 海帶來源 19
1.6 細胞株來源 19
1.7 細胞培養基 20
二、實驗方法 20
2.1 Bacillus subtilis B2活化與保存 20
2.2 海帶培養基製備 20
2.3 海帶發酵物製備與處理 20
2.4 海帶發酵物物性分析 21
2.5 海帶發酵物上清液化性分析 21
2.6 細胞培養 23
2.7 製備低密度脂蛋白 24
2.8 SDS-PAGE 膠體電泳 25
2.9 質譜分析 25
伍、結果與討論 29
一、 JFP在不同發酵時間點之物性探討 29
1.1 pH值之影響 29
1.2 黏度及水解率探討 29
二、JFP在不同發酵時間點之化性探討 30
2.1 JFP總糖變化探討 30
2.2 JFP可溶性蛋白質變化探討 30
2.3 JFP總酚含量影響抗氧化能力探討 31
2.4 JFP分子量變化探討 31
2.5 JFP單醣組成探討 33
三、JFP抑制ox-LDL誘導泡沫細胞形成之蛋白質體分析 35
3.1 巨噬細胞經ox-LDL與JFP處理後所產生的蛋白質變化 35
3.2 JFP抑制被ox-LDL上調的蛋白質體學變化 36
3.3 JFP上調被ox-LDL抑制的蛋白質體學變化 37
陸、結論 39
柒、參考文獻 41
柒、附圖 46

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