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研究生:黃郁珊
研究生(外文):HUANG, YU-SHAN
論文名稱:對家庭廢水中菌相之抗生素抗藥性增加之探討
論文名稱(外文):Explore on the increase of antibiotic resistance of bacterial phase in domestic wastewater
指導教授:許哲奇
指導教授(外文):SHU, CHE-CHI
口試委員:魏暘謝之真許哲奇
口試委員(外文):WEI, YANGHSIEH, CHIH-CHENSHU, CHE-CHI
口試日期:2020-07-21
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:化學工程與生物科技系化學工程碩士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:中文
論文頁數:41
中文關鍵詞:抗生素多種耐藥性基因水平轉移汙水處理廠
外文關鍵詞:AntibioticMultiple drug resistanceHorizontal Gene transferWaste Water Treatment Plants
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抗生素耐藥性定義為微生物在抗生素的影響下生存的能力,全球範圍內抗生素被濫用的情形,即使在衛生組織不斷警告下,仍未改善。而這一情況,導致抗生素耐藥性在微生物間傳播,也逐漸的演變成多重耐藥性,而現代醫學則加快了這轉變的過程,優勝劣敗,淘汰掉對於藥物較敏感的微生物,而自然的留下了耐藥菌繼續進行繁衍及傳播。而抗生素的殘留物抑或是衍生物會經過許多渠道流入自然環境中,其中廢水處理廠更是隱憂之一,如果廢水處理的不完善,更可能會導致廢水中擁有多重耐藥性的微生物去汙染自然界中的微生物,又因為自然環境符合許多抗生素耐藥性傳播的必要條件,因此更會加快微生物間傳播耐藥性現象的加劇。
Antibiotic resistance is defined as the ability of microorganisms to survive under the influence of antibiotics. The situation of antibiotic abuse worldwide has not improved even with constant warnings from WHO. This situation has led to the spread of antibiotic resistance among microorganisms, and gradually evolved into multi-drug resistance, and modern medicine has accelerated the process of this transformation, eliminating the microorganisms that are sensitive to drugs. Naturally, drug-resistant bacteria are left to continue multiplying and spreading. Antibiotic residues or derivatives will flow into the natural environment through many channels, and plants of wastewater treatment are one of the worries. If the wastewater treatment is imperfect, it may pollute thenature by microorganisms with multiple drug resistance. The natural environment meets many necessary conditions for the spread of antibiotic resistance, so it will accelerate the spread of resistance among microorganisms.
摘要 i
ABSTRACT ii
誌謝 iii
目錄 iv
表目錄 vii
圖目錄 viii
第1章 緒論 1
1.1 前言 1
1.2 研究背景及動機 2
第2章 文獻回顧 3
2.1 抗生素的介紹 3
2.2抗生素的機制 5
2.3抗生素耐藥性 6
2.4水平基因轉移( Horizontal Gene Transfer , HGT ) 7
2.4.1接合作用 8
2.5抗生素汙染與汙水處理廠的關係 8
2.6抗生素在環境中的影響 9
2.7 Metagenomics Next generation Sequencing ( mNGS ) 10
2.8菌種鑑定 10
2.9綠色螢光蛋白(Green Fluorescent Protein, GFP) 11
2.10抑菌圈 11
2.11 pCF10 11
第3章 研究方法 12
3.1 實驗儀器與材料 12
3.1.1 實驗儀器 12
3.1.2 藥品成分 13
3.1.3 培養基配方 14
3.1.4 藥品配方 14
3.2 實驗材料 15
3.3 實驗方法 16
3.3.1大腸桿菌轉型方法(E.coli Transformation) 16
3.3.2抽取廢水中細菌DNA 18
3.3.3 Metagenomics Next Generation Sequencing 19
3.3.4 菌種鑑定 19
3.4 實驗流程 20
3.4.1 廢水處理 20
3.4.2圓盤擴散法( 抑菌圈 ) 20
3.4.3 廢水菌之接合作用 21
3.4.4 處理後之廢水菌與MG1655 pHL 1278接合作用 21
3.4.5 菌種鑑定後之大腸桿菌與HL4195 pHL1278接合作用 22
3.4.6 多種抗藥性 23
第4章 結果與討論 27
4.1廢水菌之間的接合作用實驗 27
4.2 處理後之廢水菌與MG1655 pHL 1278接合作用實驗 28
4.3菌種鑑定 29
4.3.1 菌種鑑定後之大腸桿菌與HL4195 pHL1278接合作用 29
4.4尋找廢水菌內抗藥性基因轉移無法再現原因 30
4.4.1 圓盤擴散法 30
4.4.2 多種抗藥性 31
4.5 Metagenomics Next Generation Sequencing 33
第五章 結論 37
參考文獻 38





[1]A. Karkman, T. T. Do, F. Walsh, and M. P. J. Virta, "Antibiotic-Resistance Genes in Waste Water," Trends in Microbiology, vol. 26, no. 3, pp. 220-228, 2018/03/01/ 2018.
[2]M. Pazda, J. Kumirska, P. Stepnowski, and E. Mulkiewicz, "Antibiotic resistance genes identified in wastewater treatment plant systems – A review," Science of The Total Environment, vol. 697, p. 134023, 2019/12/20/ 2019.
[3]K. de Sousa Oliveira, L. A. de Lima, N. B. Cobacho, S. C. Dias, and O. L. Franco, "Chapter 2 - Mechanisms of Antibacterial Resistance: Shedding Some Light on These Obscure Processes?," in Antibiotic Resistance, K. Kon and M. Rai, Eds.: Academic Press, 2016, pp. 19-35.
[4]A. B. J. E. r. Boxall, "The environmental side effects of medication: How are human and veterinary medicines in soils and water bodies affecting human and environmental health?," vol. 5, no. 12, pp. 1110-1116, 2004.
[5]A. Christou et al., "The potential implications of reclaimed wastewater reuse for irrigation on the agricultural environment: The knowns and unknowns of the fate of antibiotics and antibiotic resistant bacteria and resistance genes – A review," Water Research, vol. 123, pp. 448-467, 2017/10/15/ 2017.
[6]A. K. Jindal, K. Pandya, and I. D. Khan, "Antimicrobial resistance: A public health challenge," Medical Journal Armed Forces India, vol. 71, no. 2, pp. 178-181, 2015/04/01/ 2015.
[7]About Antibiotic Resistance.
[8]E. Peterson and P. J. F. i. m. Kaur, "Antibiotic resistance mechanisms in bacteria: relationships between resistance determinants of antibiotic producers, environmental bacteria, and clinical pathogens," vol. 9, p. 2928, 2018.
[9]G. Kapoor, S. Saigal, and A. J. J. o. a. Elongavan, clinical pharmacology, "Action and resistance mechanisms of antibiotics: A guide for clinicians," vol. 33, no. 3, p. 300, 2017.
[10]W. C. J. A. m. Reygaert, "An overview of the antimicrobial resistance mechanisms of bacteria," vol. 4, no. 3, p. 482, 2018.
[11]M. Max, "Antibiotics, antibiotic resistance and environment, Encyclopedia of the Environment," Encyclopedia of the Environment, 2020.
[12]G. D. J. B. b. Wright, "Q&A: Antibiotic resistance: where does it come from and what can we do about it?," vol. 8, no. 1, p. 123, 2010.
[13]G. Mikhailovsky and R. Gordon, "Shuffling type of biological evolution based on horizontal gene transfer and the biosphere gene pool hypothesis," Biosystems, vol. 193-194, p. 104131, 2020/06/01/ 2020.
[14]R. S. McInnes, G. E. McCallum, L. E. Lamberte, and W. van Schaik, "Horizontal transfer of antibiotic resistance genes in the human gut microbiome," Current Opinion in Microbiology, vol. 53, pp. 35-43, 2020/02/01/ 2020.
[15]J. Lederberg and E. L. J. N. Tatum, "Gene recombination in Escherichia coli," vol. 158, no. 4016, pp. 558-558, 1946.
[16]D. P. Clark, N. J. Pazdernik, and M. R. McGehee, "Chapter 28 - Bacterial Genetics," in Molecular Biology (Third Edition), D. P. Clark, N. J. Pazdernik, and M. R. McGehee, Eds.: Academic Cell, 2019, pp. 896-924.
[17]A. Y.-C. Lin, T.-H. Yu, and S. K. Lateef, "Removal of pharmaceuticals in secondary wastewater treatment processes in Taiwan," Journal of Hazardous Materials, vol. 167, no. 1, pp. 1163-1169, 2009/08/15/ 2009.
[18]C. Merlin, S. Bonot, S. Courtois, and J.-C. Block, "Persistence and dissemination of the multiple-antibiotic-resistance plasmid pB10 in the microbial communities of wastewater sludge microcosms," Water Research, vol. 45, no. 9, pp. 2897-2905, 2011/04/01/ 2011.
[19]I. Michael et al., "Urban wastewater treatment plants as hotspots for the release of antibiotics in the environment: A review," Water Research, vol. 47, no. 3, pp. 957-995, 2013/03/01/ 2013.
[20]K. Bondarczuk, A. Markowicz, and Z. Piotrowska-Seget, "The urgent need for risk assessment on the antibiotic resistance spread via sewage sludge land application," Environment International, vol. 87, pp. 49-55, 2016/02/01/ 2016.
[21]A. Lupo, S. Coyne, and T. U. J. F. i. m. Berendonk, "Origin and evolution of antibiotic resistance: the common mechanisms of emergence and spread in water bodies," vol. 3, p. 18, 2012.
[22]S. Yohe and B. Thyagarajan, "Review of Clinical Next-Generation Sequencing," vol. 141, no. 11, pp. 1544-1557, 2017.
[23]O. Shimomura, F. H. Johnson, Y. J. J. o. c. Saiga, and c. physiology, "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea," vol. 59, no. 3, pp. 223-239, 1962.
[24]陳. 陳逸民, "綠色螢光蛋白的發現,讓研究活體蛋白質的工作變得容易許多。," 科學發展, no. 440, pp. 60-65, 2009.
[25]M. Balouiri, M. Sadiki, and S. K. Ibnsouda, "Methods for in vitro evaluating antimicrobial activity: A review," Journal of Pharmaceutical Analysis, vol. 6, no. 2, pp. 71-79, 2016/04/01/ 2016.
[26]T. Bae and G. M. J. M. m. Dunny, "Dominant‐negative mutants of prgX: evidence for a role for PrgX dimerization in negative regulation of pheromone‐inducible conjugation," vol. 39, no. 5, pp. 1307-1320, 2001.
[27]E. Caserta et al., "In vivo and in vitro analyses of regulation of the pheromone-responsive prgQ promoter by the PrgX pheromone receptor protein," vol. 194, no. 13, pp. 3386-3394, 2012.
[28]Y. Chen et al., "Mechanisms of peptide sex pheromone regulation of conjugation in Enterococcus faecalis," vol. 6, no. 4, p. e00492, 2017.
[29]姚文成. (2014). 小水滴的公共浴場 - 迪化污水處理廠.
[30]J. Jutkina, C. Rutgersson, C.-F. Flach, and D. J. J. S. o. t. T. E. Larsson, "An assay for determining minimal concentrations of antibiotics that drive horizontal transfer of resistance," vol. 548, pp. 131-138, 2016.
[31]T. Vesth et al., "Veillonella, Firmicutes: microbes disguised as Gram negatives," vol. 9, no. 2, pp. 431-448, 2013.


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