隱形眼鏡產品已經在我們的生活普及，但配戴過程中還是有許多問題。在配戴隱形眼鏡後會破壞淚液平衡並且與眼部組織接觸，過程中會與眼瞼與眼表產生摩擦運動。當我們戴上隱形眼鏡，淚液蛋白吸附鏡片會破壞淚膜平衡，造成許多不適以及後遺症，並且會提高淚液中水分蒸發而造成乾眼問題，這會導致摩擦刺激結膜而引起過敏反應與不適感，許多研究表明隱形眼鏡的配戴舒適度與鏡片在人眼中的摩擦係數有相關。最近，溶菌酶與隱形眼鏡的相互作用受到大家關注，除了溶菌酶，淚液中還有許多蛋白質。文獻指出白蛋白在眼中濃度變化很大，在乾眼症狀發生初期，白蛋白在淚液中濃度明顯上升，因此白蛋白在淚液中濃度的改變具有臨床意義，目前白蛋白對隱形眼鏡的交互作用和摩擦性質尚不明瞭。為了瞭解白蛋白對不同材質軟式隱形眼鏡的摩擦性質之影響，本研究利用體外測試隱形眼鏡摩擦潤滑性質的方法與蛋白質定量測試方法，探討白蛋白溶液對四種市售軟式隱形眼鏡摩擦情形的影響，量化不同材質隱形眼鏡之摩擦係數與白蛋白的吸附情形，來了解軟式隱形眼鏡對白蛋白的摩擦行為。

Contact lens products have become popular in our lives, and silicone gel lenses are also popular because of the comfort of wearing. After wearing contact lenses, it will destroy the tear balance and contact with eye tissues, and will cause frictional movement with the eyelid and eye surface during blinking. When we put on contact lenses, the tear protein adsorption lens will disrupt the tear film balance, causing many discomforts and sequelae, and will increase the evaporation of water in the tears and cause dry eyes. This will cause friction to stimulate the conjunctiva and cause allergic reactions and discomfort. Many Studies have shown that the wearing comfort of contact lenses is related to the coefficient of friction of lenses in human eyes. Recently, the interaction between lysozyme and contact lenses has attracted attention. In addition to lysozyme, there are many proteins in tears. The literature pointed out that in the early stage of dry eye symptoms, the concentration of albumin in tears increased significantly, so the change in the concentration of albumin in tears has clinical significance. At present, the interaction and friction properties of albumin on contact lenses are not clear. Therefore, in order to understand the friction properties of albumin on soft contact lenses of different materials, this study used in vitro test contact lens friction lubrication platform and protein quantitative test method to explore the effect of albumin solution on the friction effect of various commercially available soft contact lenses, Quantify the friction coefficient of different contact lenses and albumin adsorption to understand the friction behavior of soft contact lenses on albumin.

摘 要 i
ABSTRACT ii
誌 謝 iv
目 錄 v
表目錄 vii
圖目錄 viii
第 一 章 序論 1
1.1 前言 1
1.2 研究動機 2
第 二 章 文獻回顧 3
2.1 隱形眼鏡 3
2.1.1 水凝膠與矽水凝膠 3
2.1.2 軟式隱形眼鏡材質的演變 5
2.2 淚膜 7
2.3 軟性隱形眼鏡在眼表組織中的運動行為 8
2.4 蛋白質的溶解與沉澱 9
2.4.1雙電層與水化層的形成 9
2.4.2蛋白質的沉澱 9
2.5 蛋白質吸附與軟式隱形眼鏡摩擦係數的影響 10
2.5.1 蛋白質與生醫材料的吸附 10
2.5.2 蛋白質吸附鏡片後的結構變化 11
2.5.3 蛋白質對鏡片摩擦性質的相關研究 12
2.6 白蛋白 13
2.6.1 白蛋白在淚膜中的臨床意義與潛力 14
2.6.2 白蛋白的吸附研究進展 15
第 三 章 關鍵問題與研究方法 16
3.1 關鍵問題 16
3.1.1配戴隱形眼鏡引發之不適感 16
3.1.2白蛋白在淚膜中的特性 16
3.2 研究方法 17
3.2.1 摩擦實驗之隱形眼鏡載具 17
3.2.2 四種不同廠牌之軟式隱形眼鏡 18
3.2.3 實驗架構 18
第 四 章 實驗方法與材料 19
4.1 摩擦實驗 19
4.1.1 目的 19
4.1.2 實驗儀器 19
4.1.3 軟式隱形眼鏡摩擦平台 20
4.1.4 實驗材料及藥品 22
4.1.5 實驗步驟 23
4.2 白蛋白吸附實驗 24
4.2.1 目的 24
4.2.2 分光光度檢測法 24
4.2.3 利用紫外光-可見光光譜儀進行白蛋白定量 25
4.2.4 實驗材料及藥品 25
4.2.5實驗步驟 26
第 五 章 結果與分析 27
5.1 軟式隱形眼鏡材質的表面形貌 27
5.2 四種材質軟性隱形眼鏡之白蛋白吸附實驗結果 29
5.3 四種軟式隱形眼鏡材質與不同潤滑液之摩擦結果 31
5.3.1 摩擦係數量化分析 44
5.4 鏡片先吸附白蛋白後再進行摩擦之實驗結果 50
第 六 章 討論 57
第 七 章 結論 61
參考文獻 62

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