臺灣博碩士論文加值系統

目錄.....................................................i
摘要....................................................iii
Abstract.................................................iv
1.緒論....................................................1
1.1口型蛋白質(Stomatin)....................................1
1.2口型蛋白的分布與功能.....................................1
1.3脂肪組織與脂肪新生(Adipogenesis)........................2
1.4細胞外調節蛋白激酶(extracellular signal‑regulated kinases ,ERK).....................................................3
1.5表皮生長因子接受器(EGFR) 與表皮生長因子接受器內化作用(EGFR internalization)..........................................4
2.實驗材料與方法...........................................6
2.1細胞培養與3T3-L1脂肪前驅細胞分化.......................6
2.2架構質體(plasmid)和細胞轉染(transfection).............6
2.3慢病毒(Lentivirus)系統與感染3T3-L1 脂肪前驅細胞........7
2.4共軛交顯微鏡螢光影像..................................8
2.5西方墨點與分析........................................9
2.6油紅O (Oil Red O)染色與定量油滴累積方法................9
2.7生長因子接受器(EGFR)降解實驗............................10
2.8 MAPK pathway 抑制劑刺激實驗...........................11
2.9 Reverse transcription PCR (RT-PCR) 與 Real-time PCR (Quantitative PCR,qPCR ).................................11
2.10統計分析..............................................12
3.結果...................................................13
3.1降低口型蛋白質表達可增加脂肪分化後期ERK的活化作用.........13
3.2口型蛋白降低所調控的ERK的活化可能具有調控分化之能力.......13
3.3口型蛋白與表皮生長因子接受器之相關性.....................14
3.4口型蛋白無法調控表皮生長因子接受器之降解作用..............15
3.5口型蛋白調控表皮生長因子接受器之內化作用（Internalization）......................................16
4. 討論..................................................20
4.1探討口型蛋白質敲弱組別ERK活化上升與MAPK路徑抑制劑實驗結果...20
4.2探討口型蛋白質與表皮生長因子接受器的關聯性................21
5.參考文獻................................................24
6.圖.....................................................26
7.附錄...................................................63
附錄表格一...............................................63
附錄表格二...............................................64
附錄圖表.................................................65

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