臺灣博碩士論文加值系統

本研究以乾式球磨法及助研磨劑碳酸鈉製備碳量子點（Carbon quantum dots, CQDs），碳源的選擇導電碳黑（KJ），有著優異的特性，其粒徑小且呈球形的外貌，使其更容易製備出碳量子點。
本研究所製備碳量子點的粒徑約為3 nm，當激發光源波長為320 nm時，在激發光波長430 nm會有強度最強的藍綠色螢光，其螢光量子產率約為2.23 %。碳量子點經過透析後，鈉的含量僅剩0.7 %，在以海拉癌細胞（HeLa cells）進行毒性檢測時，得知碳量子點在200 μg / ml濃度下，HeLa cells仍有超過90 %的存活率，在365 nm紫外光下可以看到綠光的細胞輪廓。

We report a cost-effective ball-milling method for producing CQDs using a mixture of conductive carbon black (KJ) as the carbon source and sodium carbonate as an abrasive. The results show that the proposed exfoliation method produces CQDs with diameters 3 nm. When using 320 nm ultraviolet light, there will be a maxima blue light at 430 nm. The quantum yield is about 2.23 %. After dialysis, the CQDs only retain about 0.7 %. CQDs is low toxicity to HeLa cancer cells, with a survival rate of more than 90% at a concentration of 200 μg/ml. Because carbon quantum dots have the characteristics of stable light emission, small particle size and low toxicity, they are suitable for use in the visualization of biological cells.

中文摘要 I
Abstract II
目錄 III
圖目錄 IV
第一章、緒論 1
第二章、實驗方法 4
第三章、結果與討論 11
第四章、結論 24
參考文獻 25
附錄 30

El-Eskandarany (Ed.), Mechanical Alloying, William Andrew Publishing, Oxford, 2015, pp. 13-47.
[37] J.S. Lin, Y.W. Tsai, K. Dehvari, C.C. Huang, J.Y. Chang, A carbon dot based theranostic platform for dual-modal imaging and free radical scavenging, Nanoscale 11(43) (2019) 20917-20931.
[38] S. Kang, K.M. Kim, Y. Son, S. Mhin, J.H. Ryu, K.B. Shim, B. Lee, H. Han, T.J.S.r. Song, Graphene oxide quantum dots derived from coal for bioimaging: facile and green approach, Sci Rep 9(1) (2019) 1-7.
[39] T. Xing, T. Ramireddy, L.H. Li, D. Gunzelmann, H. Zeng, W. Qi, S. Zhou, Y.J.P.C.C.P. Chen, Lithium storage in disordered graphitic materials: a semi-quantitative study of the relationship between structure disordering and capacity, Physical Chemistry Chemical Physics 17(7) (2015) 5084-5089.
[40] B.Y. Han, M.B. Yu, T.T. Pen, Y. Li, X.X. Hu, R.C. Xiang, X.F. Hou, G.H. He, One-step extraction of highly fluorescent carbon quantum dots by a physical method from carbon black, New J Chem 41(13) (2017) 5267-5270.
[41] X. Feng, F. Zhang, Y. Wang, Y. Zhang, Y. Yang, X.J.A.P.L. Liu, Luminescent carbon quantum dots with high quantum yield as a single white converter for white light emitting diodes, Applied Physics Letters 107(21) (2015) 213102.
[42] M. Perikala, A.J.A.o. Bhardwaj, Highly stable white-light-emitting carbon dot synthesis using a non-coordinating solvent, Acs Omega 4(25) (2019) 21223-21229.
[43] E. ISO, ISO 10993-5: biological Evaluation of Medical Devices-Part 5: Tests for in vitro Cytotoxicity, International Organization for Standardization 10993-5:2009 ed (1999).
[44] R.I. Amann, In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes, in: A.D.L. Akkermans, J.D. Van Elsas, F.J. De Bruijn (Eds.), Molecular Microbial Ecology Manual, Springer Netherlands, Dordrecht, 1995, pp. 331-345.