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研究生:徐嘉蔚
研究生(外文):HSU, CHIA-WEI
論文名稱:生產抗神經壞死病毒 (NNV) 殼體蛋白之類單株多株抗體配合 光纖式粒子電漿共振生物感 生產抗神經壞死病毒 (NNV) 殼體蛋白之類單株多株抗體配合 光纖式粒子電漿共振生物感 (Fiber-Optic Particle Plasmon Resonance)檢測系統
論文名稱(外文):Production of monoclone-like polyclonal antibody against coatprotein of Nervous Necrosis Virus (NNV) for fiber optic plasmonresonance (FOPPR) detection system
指導教授:陳浩仁周禮君周禮君引用關係
指導教授(外文):Chen, Hau-RenChau, Lai-Kwan
口試委員:周禮君黃憲斌王少君
口試委員(外文):Chau, Lai-KwanHUANG,HSIEN-BINWang, Shau-Chun
口試日期:2020-07-29
學位類別:碩士
校院名稱:國立中正大學
系所名稱:生命科學系生物醫學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2021
畢業學年度:109
語文別:中文
論文頁數:82
中文關鍵詞:石斑魚神經壞死病毒生物感測器抗體
外文關鍵詞:FOPPRNervous Necrosis Virus (NNV)Immunosensor
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  • 點閱點閱:125
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1 緒論 8
1.1 台灣石斑魚養殖概況 8
1.2 病毒性神經壞死症之病史與病徵 9
1.3 神經壞死病毒 (Nervous Necrosis Virus, NNV)病毒介紹 10
1.4 神經壞死病毒檢測方法 11
1.5 光纖式粒子電漿共振檢測系統(Fiber optic particle plasmon resonance system, FOPPR) 13
1.6 線性陣列抗原決定區(Linear array epitope technique, LAE) 15
1.7 研究目的 16
2 實驗方法 17
2.1 實驗流程 17
2.2 實驗材料 17
2.2.1 生物材料 17
2.2.2 藥品、試劑 18
2.2.3 實驗儀器 19
2.3 轉型作用 (Transformation) 22
2.4 LAE抗原表現與純化 (Expression and purification of LAE antigen) 23
2.4.1 蛋白質小量表現(Protein expression with Small scale) 23
2.4.2 蛋白質大量表現 (Protein expression with large scale) 23
2.4.3 蛋白質分析 24
2.4.4 蛋白質純化 (Protein purification) 25
2.4.5 蛋白質定量 26
2.5 LAE多株抗體生產 (LAE Polyclonal antibody production) 26
2.6 LAE多株抗體純化 (LAE Polyclonal antibody purification) 26
2.7 LAE抗體性質分析 (properties analysis of LAE antibody) 27
2.7.1 酵素免疫吸附分析 (enzyme-linked immunosorbent assay, ELISA) 27
2.7.2 西方墨點法 (Western blotting) 27
2.7.3 FOPPR 28
2.7.3.1 於感測晶片中修飾抗體步驟 28
2.7.3.2 以FOPPR對NNV coat protein進行多濃度檢測 28
2.7.3.3 以FOPPR對NNV病毒液進行多濃度檢測 29
2.8 GF-1細胞株繼代 29
2.9 NNV 病毒株擴增 29
2.10 50%病毒感染效價 (50% Tissue Culture Infective Dose, TCID50/mL) 30
3 實驗結果 30
4 討論 35
5 未來展望 38
6 參考文獻 39
7 圖表 47
8 附圖表 71

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