臺灣博碩士論文加值系統

本論文藉由編織技術及靜電紡絲技術製備出具有導電性及降解性之聚乙烯醇/奈米碳管神經導管。實驗中以紗線加撚製備出不同撚數之聚乙烯醇紗線，並透過編織技術製備出中空編織物，再經由綠槴子素對其進行交聯，最後以其機械特性、表面觀察及生物特性進行評估，在神經導管結構中作為主要支撐結構及機械強力提供。以靜電紡絲技術製備可導電之聚乙烯醇/奈米碳管靜電紡絲薄膜，並對其進行纖維直徑、導電性、親水性及生物特性等測試評估。由測試結果中可以得知，3 truns/ intch的聚乙烯醇中空編織物及奈米碳管含量為0.25 wt%之聚乙烯醇/奈米碳管有較佳的測試結果，並將其在進行複合，製備出聚乙烯醇/奈米碳管神經導管，並針對其機械特性、導電性及生物特性進行評估。由研究成果中可以得知聚乙烯醇/奈米碳管神經導管拉伸強力為21.9 N，且內層聚乙烯醇/奈米碳管靜電紡絲薄膜具有較低電阻值為9.2 ohm及水接觸角為74.2°，經由降解測試及體外細胞測試中可以得知，神經導管在五周內有穩定的降解速率約為0.85-0.9；與細胞共培養三天後細胞存活數量為對照組之1.03倍，即聚乙烯醇/奈米碳管神經導管之結構及設計符合神經導管之應用。

In this study, braiding technique and electrostatic spinning are employed to produce polyvinyl alcohol (PLA)/carbon nanotubes (CNT) nerve conduits. For the starter, PLA yarns are twisted at different twist counts, after which the PLA twisted yarns are braided into hollow tubular braids. Next, genipin is added to crosslink with PVA braids. The final materials are tested for mechanical properties, surface observation, and physical properties, thereby providing the nerve conduits with a support structure and better mechanical properties. In addition, electrostatic spinning is conducted to form conductive PLA/CNT electrospinning membranes that are then evaluated for the fiber diameter, electrical conductivity, hydrophilicity, and biological attributes. The test results indicate that the optimal PLA tubes are made with a twist count of 3 turns/inch while the optimal membranes are made of 0.25 wt% of CNT. Hence, the two parameters are combined to produce PLA/CNT nerve conduits, and the mechanical properties, conductivity, and biological attributes are evaluated. PLA/CNT nerve conduits exhibit tensile strength of 21.9 N, meanwhile, the internal PLA/CNT electrospinning membranes demonstrate a lower electrical resistivity of 9.2 ohm and a water angle being 74.2°. According to the biodegradation test and in vitro measurement, PLA/CNT nerve conduits have a stabilized degradation rate being15-20 % as well as 1.03 times greater cell survival counts greater than the control group. With the proposed structure and design, PLA/CNT nerve conduits are proved to be a good candidate for the use of nerve conduits.

目錄
第一章 前言 1
1.1 周圍神經 1
1.2 周圍神經的損傷 2
1.3 神經再生及修復 4
1.4 組織工程 7
1.5 神經導管 10
1.6 文獻回顧 14
1.7 相關專利 18
1.8 研究目的 25
第二章 製程設計 27
2.1 神經導管材料 27
2.2 神經導管製程 32
2.2.1中空編織技術 32
2.2.2靜電紡絲 35
2.3交聯機制 39
2.4神經導管特性評估 40
第三章 實驗 46
3.1實驗材料 46
3.2實驗儀器 47
3.3實驗流程說明 49
3.4測試項目 56
3.5實驗參數設計 61
第四章 結果與討論 63
4.1雙股聚乙烯醇撚紗之表面觀察及拉伸強力測試。 63
4.2 聚乙烯醇中空編織物在交聯前後之表面觀察 67
4.3 聚乙烯醇中空編織物之傅立葉變換紅外光譜(FTIR) 70
4.4 聚乙烯醇中空編織物之機械強度 72
4.5 PVA中空編織物之降解率 74
4.6 聚乙烯醇/奈米碳管靜電紡絲溶液之黏度及電導度 76
4.7 聚乙烯醇/奈米碳管靜電紡絲薄膜之表面觀察及纖維尺寸分析 78
4.8 PVA/CNT 靜電紡絲薄膜之傅立葉變換紅外光譜(FTIR) 81
4.9 PVA/CNT 靜電紡絲薄膜之水接觸角 82
4.10聚乙烯醇/奈米碳管靜電紡絲薄膜之導電性 84
4.11 聚乙烯醇/奈米碳管靜電紡絲薄膜之降解率影響 86
4.12 聚乙烯醇/奈米碳管靜電紡絲薄膜之細胞相容性 88
4.13 聚乙烯醇/奈米碳管神經導管之拉伸強度 91
4.14 聚乙烯醇/奈米碳管神經導管之表面電阻 93
4.15 聚乙烯醇/奈米碳管神經導管之降解測試 94
4.16 聚乙烯醇/奈米碳管神經導管之細胞存活率 95
第五章 結論 97
參考文獻 98

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