臺灣博碩士論文加值系統

本論文提出一種基於高分子與液晶複合材料的生物感測手段，可應用於牛血清蛋白之檢測。經過紫外光曝照高分子聚合後，實驗結果顯示吾人得以達到更佳之偵測極限及偵測靈敏度，背後的機制是摻雜低濃度光固化高分子預聚物於液晶中表現出特殊的預傾角調控特性及折射率不匹配導致的光散射。本研究採用高分子/液晶複合系統做為牛血清蛋白偵測手段，搭配介電常數的量測進行牛血清蛋白濃度定量分析，再利用介電常數值換算出液晶等效傾角後，該等效傾角與牛血清蛋白的一對一關係擬合出線性函數來做為定量分析的依據，此偵測手段所達最佳偵測極限為10-10 g/ml。

We propose to LC–prepolymer (E7–NOA65) as the sensing element for protein detection. After irradiating ultraviolet light to perform the photopolymerization process, our results indicate that the limit of detection can be promoted due to the enhancement of the optical signal, derived from the controlling of the LC’s pretilt angle and light scattering. In this study, the polymer–liquid crystal composite system was used as the detection method of bovine serum protein, combined with the measurement of dielectric constant for quantitative analysis of the concentration of bovine serum protein, and then the dielectric constant value was used to calculate the equivalent tilt angle of the liquid crystal. The one-to-one relationship between tilt angle and the concentration of bovine serum protein is fitted to a linear function as a basis for quantitative analysis. The best detection limit reached by this method is 10-10 g/ml.

摘　　　要 i
ABSTRACT ii
目　　　錄 iii
表　目　錄 v
圖　目　錄 vi
第1章 緒論 1
1.1 前言 1
1.2 液晶簡介 4
1.3 研究動機 7
1.4 論文架構 8
第2章 基礎背景 10
2.1 液晶生物感測沿革 10
2.2 高分子預聚物與液晶之複合材料 20
2.3 以光固化預聚物調控液晶預傾角 23
2.4 介電頻譜 25
2.4.1　物質的極化與介電 25
2.4.2　液晶的介電常數 27
第3章 樣品配製與實驗裝置 29
3.1實驗材料 29
3.2 實驗儀器 35
3.3 樣品製備 36
3.3.1 基板清洗與浸鍍配向膜 36
3.3.2 牛血清蛋白（BSA）附著之液晶合製作 37
3.4 實驗量測 40
3.4.1 牛血清蛋白之光學紋理觀測 40
3.4.2 介電實部數值之測量 41
3.4.3 圖像分析軟體ImageJ 43
第4章 實驗結果與討論 44
4.1 無電極液晶盒之光學紋理觀測 45
4.1.1 液晶與高分子前驅物生物檢測平台之材料參數選擇 45
4.1.2 蛋白質之檢測結果 48
4.2　ITO液晶盒之介電頻譜量測 57
4.2.1 光學玻璃與ITO玻璃對 UV光之吸收差異 57
4.2.2以介電實部訊號定量蛋白質濃度 59
第5章 結論與未來展望 66
參考文獻 69

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