臺灣博碩士論文加值系統

目錄…………I
圖目錄…………III
縮寫表…………IV
摘要…………V
Abstract…………VI
第一章 緒論…………1
第一節 肝癌…………1
一、肝癌（Liver cancer）…………1
二、肝癌與肥胖…………1
三、肝癌與二乙基亞硝胺(diethylnitrosamine ,DEN)和四氯化碳(CCl4)…………2
四、肝癌與IL-6…………3
五、肝癌與TNF-α…………5
第二節 Microsomal triglyceride transfer protein(MTP)…………5
第三節Proline-rich acidic protein 1(PRAP1)…………6
第二章 實驗材料與方法…………9
第一節 實驗材料…………9
一、藥品試劑…………9
二、實驗儀器…………11
三、溶液配置…………12
第二節 實驗方法…………15
一、小鼠…………15
二、基因型分型(genotyping)…………16
三、石蠟切片…………18
四、Hematoxylin and eosin (H&E) 染色…………19
五、冷凍切片…………20
六、Oil Red O 染色…………20
七、酵素免疫分析法Enzyme-linked immunosorbent assay (ELISA)…………21
八、統計分析…………21
第三章 結果…………22
第一節 HFD誘導小鼠肥胖之模型…………22
一、建立基因剔除小鼠…………22
二、長期餵食HFD，PRAP1的缺乏不影響老鼠體重變化……………………22
三、只餵食HFD的小鼠不會自發性誘導肝臟腫瘤…………23
第二節 DEN與HFD誘導HCC之小鼠模型…………23
一、DEN與HFD誘導的HCC模型中，PRAP1缺失不影響體重變化…………23
二、DEN與HFD誘導的HCC模型中，PRAP1缺失不影響腫瘤發生…………24
第三節 使用DEN、CCl4與HFD誘發小鼠肝癌之模型…………25
一、小鼠模型的時間調整…………25
二、PRAP1-/-母鼠在DEN+CCl4+HFD處理的模型中腫瘤狀況有減輕的趨勢…………25
第四節 以DEN與餵食Chow diet誘發小鼠腫瘤發生之模型…………26
一、建構Chow diet + DEN小鼠模型…………26
二、Chow diet + DEN小鼠模型中PRAP1 缺失不影響腫瘤發展…………27
第四章 結論…………28
第五章 討論…………28
第六章 參考文獻…………30

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