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研究生:葉佳旻
研究生(外文):Yeh, Chia-Min
論文名稱:探討神經醯胺對CaMKII相關訊息傳遞路徑和學習記憶的影響
論文名稱(外文):Effects of Ceramides on CaMKII-related Signaling, Learning, and Memory
指導教授:林炎壽
指導教授(外文):Lin, Yenshou
口試委員:王慈蔚梁美智林炎壽
口試委員(外文):Wang, Tsu-WeiLiang, Mei-ChihLin, Yenshou
口試日期:2021-06-24
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2021
畢業學年度:109
語文別:中文
論文頁數:53
中文關鍵詞:神經醯胺CaMKII阿茲海默氏症學習與記憶
外文關鍵詞:ceramidesCaMKIIAlzheimer's diseaseLearning and memory
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第一章 緒論 1
一、神經醯胺 1
二、阿茲海默症 2
三、阿茲海默症之模式小鼠J20 3
四、記憶相關的訊息傳遞路徑 4
五、表觀遺傳相關之蛋白質 5
第二章 研究動機與目的 6
第三章 研究材料與實驗方法 7
一、藥劑 7
二、小鼠大腦皮質神經細胞初級培養 7
三、細胞免疫染色 (Immunocytochemistry, ICC) 8
四、蛋白質萃取、定量 8
五、電泳、西方墨點法 9
六、Fura-2 AM(Acetoxy-methyl-ester Fura-2)鈣離子染色實驗 10
七、基因型檢測 10
八、實驗動物與藥物給予 11
九、莫氏水迷津測試 (Morris water maze test) 11
十、開放空間測試 (Open field test) 12
十一、高架十字迷宮 (Elevated plus maze, EPM) 13
十二、腹腔注射葡萄糖耐受性分析 (Intraperitoneal glucose tolerance test, IPGTT) 13
十三、臉頰採血檢測血清中的GOT、GPT 13
十四、灌流(Perfusion)和肝臟、腎臟組織H&E染色 14
十五、統計分析 14
第四章 研究結果 15
一、神經醯胺可增加神經細胞中CaMKII之磷酸化 15
二、神經醯胺亦可增加CREB和Erk之磷酸化 15
三、神經醯胺於刺激的時間內未能觀察到細胞中的鈣離子濃度增加 16
四、神經醯胺具有可增加初級培養神經細胞中H3K4甲基化和Egr1表現量之趨勢 17
五、離乳後給予神經醯胺並不會影響小鼠的體重和成年後的一些生理參數 17
六、離乳後給予J20小鼠神經醯胺不會改善小鼠的焦慮行為 19
七、離乳後給予神經醯胺可以改善J20小鼠的空間記憶行為 20
第五章 討論 22
參考文獻 25
圖表 33
附錄 51
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