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研究生:張哲榮
研究生(外文):Chang, Che-Jung
論文名稱:基於影像處理之斑馬魚運動軌跡分析
論文名稱(外文):Motion Trajectory Analysis of Zebrafish Based on Image Processing
指導教授:賀耀華林豊益
指導教授(外文):Ho, Yao-HuaLin, Li-Yih
口試委員:周銘翊陳伶志方瓊瑤賀耀華林豊益
口試日期:2021-10-12
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:資訊工程學系
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:2021
畢業學年度:109
語文別:中文
論文頁數:49
中文關鍵詞:斑馬魚行為影像處理MEIMHI
外文關鍵詞:Image ProcessingMotion Object TrackingZebrafish
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  • 下載下載:10
  • 收藏至我的研究室書目清單書目收藏:0
在過去的研究中,發現斑馬魚的基因與人類相近,其基因序列中可找到約八成與人類疾病相關的基因,且斑馬魚可以迅速繁殖,相較於過往實驗用的白老鼠所花費成本更低 。 在神經行為學的研究上科學家透過斑馬魚的行為,了解斑馬魚中樞神經系統的運作 不過由於斑馬魚個體間外型相近,在進行實驗時 科學家不易辨識分析 追蹤個體 且在 過去 研究上 都是利用肉眼觀看實驗過程 ,或是透過觀看錄影影片觀察斑馬魚行為,此過程須花費不少人力和時間成本,且使用者無法同時進行觀看多種斑馬魚實驗。
本研究利用影像動態追蹤的技術,運用運動歷史圖 ( Motion History Image, MHI ) 演算法提出一套斑馬魚影像分析系統 Fish Motion Tracking (FMT) System 且提出一個提高追蹤精準度的方法-方向向量推算法,此方法將用於斑馬魚影像重疊後,如何辨別追蹤斑馬魚個體為何 FMT 系統會將斑馬魚的影像數據轉換成實驗數據 (斑馬魚座標和游動方向向量 科學家可以利用這些數據分析斑馬魚游動與行為,進而減少過去研究中所需花費的人力和時間成本。
According to the previously conducted research, Zebrafish is a popular organism and widespread use for the study of vertebrate gene function and human genetic disease due to the similarity to human. In fact, the gene sequence of Zebrafish has approximately 80% related to human diseases. Therefore, Zebrafish are more useful than Albino mice to research experiments. In the Neuroethology of Zebrafish’s research, scientists use Zebrafish behavior to understand the operation of the Zebrafish’s Central Nervous System. However, due to the similar appearance of Zebrafish individuals, it is difficult for scientists to identify, analyze, and track individuals during experiments. In the past, the conducted experiments on the behavior of Zebrafish often use naked eyes or videotape to monitor the results. Therefore, a lot of labor and time are required to conduct multiple experiments. Also, it is difficult to analyze individual Zebrafish’s behavior simultaneously due to similar body shapes. In this research, we proposed the Fish Motion Tracking (FMT) system to dynamically object tracking a set of Zebrafish. By taking advantage of the image process approach, we are able to improve the accuracy of the tracking result with the Motion History Image (MHI) algorithm and Direction Vector Inference (DVI) algorithm. The contributions of the proposed FMT system are as follow: 1) it improves the accuracy of the tracking results of Zebrafish; 2) the system is able to identify the individual Zebrafish after multiple Zebrafish overlap each other; and 3) the FMT system converts the Zebrafish image data into experimental data (Zebrafish coordinates and swimming direction vectors) which scientists can use these data to analyze Zebrafish behavior by the individual of the Zebrafish data. The proposed FMT system can greatly reduce the labor and time costs required in the related research.
第一章 緒論 1
第一節 研究背景 1
第二節 研究動機 2
第三節 研究目的 3
第二章 文獻探討 4
第一節 斑馬魚行為分析 4
2.1.1 斑馬魚的游動行為 4
2.1.2 斑馬魚的社交行為 4
2.1.3 斑馬魚的追逐行為 5
2.1.4 斑馬魚的群游行為 5
2.1.5 斑馬魚的恐懼行為 5
第二節 斑馬魚軌跡分析 6
2.2.1 idTracker 6
2.2.2 Tracktor 7
第三節 影像閾值處理 7
2.3.1 二值化處理 ( Binary Thresholding ) 7
2.3.2 自適應閾值處理 ( Adaptive Thresholding ) 8
2.3.3 大津法 ( OTSU Thresholding ) 9
第四節 影像形態學 11
2.4.1 侵蝕 (Erosion) 11
2.4.2 膨脹 ( Dilation ) 12
2.4.3 開運算 ( Opening ) 13
2.4.4 閉運算 ( Closing ) 14
第五節 影像動態追蹤 14
第三章 研究方法 17
第一節 系統流程 17
第二節 系統初始化 18
第三節 影像動態偵測 19
3.3.1 影像幀間差法 19
3.3.2 運動能量圖 ( Motion Energy Image ) 20
3.3.3 運動歷史圖 ( Motion History Image ) 21
3.3.4 計算 MHI 影像特徵 22
第四節 影像數據分析 23
3.4.1 斑馬魚輪廓偵測 23
3.4.2 輪廓與 MHI 特徵數據做比對 24
3.4.3 檢查追蹤與校正 25
3.4.4 消失的斑馬魚偵測 26
3.4.4.1 伽馬校正 ( Gamma correction ) 27
3.4.5 哈根巴赫-比紹夫數額 ( Hagenbach-Bischoff Quota ) 29
3.4.6 匈牙利演算法 ( Hungarian Algorithm ) 30
第四章 實驗與分析 34
第一節 實驗設定 34
第二節 精準度結果 35
第三節 方向向量推算法結果 38
第四節 系統校正 41
第五節 開源軟體比較 42
第五章 結論與未來展望 45
參考文獻 46
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