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研究生:林冠宇
研究生(外文):Lin, Kuan-Yu
論文名稱:杜鵑花酸提升間葉幹細胞旁分泌效應調節抗老化因子miRNA-7850-5p 延緩皮膚衰老
論文名稱(外文):Azelaic acid increases paracrine effect on mesenchymal stem cells to regulate anti-senescent marker miRNA-7850-5p in aging skin
指導教授:陳冬生陳冬生引用關係
指導教授(外文):Chen, Tung-Sheng
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:生技醫藥產業碩士學位學程
學門:醫藥衛生學門
學類:藥學學類
論文出版年:2020
畢業學年度:109
語文別:中文
論文頁數:90
中文關鍵詞:杜鵑花酸幹細胞皮膚老化微小分子核糖核酸
外文關鍵詞:Azelaic acidStem cellsSkin AgingMicroRNA
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目錄 1
中文摘要 6
Abstracts 8
1.緒論 10
1-1皮膚 10
1-2皮膚光老化 11
1-3 microRNA(miRNA)調控老化機制 14
1-4目前皮膚抗老化的治療方式 16
1-5幹細胞用來改善皮膚老化的基本原理 17
1-6幹細胞種類 20
1-7幹細胞強化 20
1-8杜鵑花酸(Azelaic acid,AzA) 22

2.研究動機 25

3.研究材料與方法 26
3-1 化學藥品與試劑 26
3-2 人類角質細胞(Keratinocytes、HaCaT)培養 26
3-3 臍帶膠質間葉幹細胞(Wharton's Jelly Stem Cells,WJSC) 27
3-4 雙氧水(Hydrogen peroxide,H2O2)傷害HaCaT 27
3-5 AzA強化幹細胞 28
3-6 細胞存活率檢測(MTT assay) 28
3-7 蛋白質定量(細胞) 29
3-8 蛋白質定量(動物組織) 29
3-9 西方墨點法檢測(Western blot assay)(細胞) 30
3-10 西方墨點法檢測(Western blot assay) (動物組織) 31
3-11 小分子干擾核糖核酸(small interfering RNA,siRNA)轉染WJSC 33
3-12 miRNA轉染HaCaT 33
3-13 流式細胞儀分析(Flow Cytometry) 34
3-14 細胞分組 35
3-15 實驗動物 35
3-16 動物分組 35
3-17 皮膚老化動物模式的誘發 36
3-18 馬森三色染色法(Masson's trichrome stain) 36
3-19 病理組織化學染色(Hematoxylin and eosin stain、H&E stain) 37
3-20 病理組織免疫染色(Immunohistochemistry, IHC) 37
3-21 統計分析 38

4.實驗結果 39
4-1用H2O2誘導 HaCaT細胞老化細胞存活率檢測 39
4-2用H2O2誘導 HaCaT細胞老化西方墨點法檢測 39
4-3 miRDB網站搜尋miRNA 40
4-4 miRNA-7850-5p 短暫轉殖後西方墨點法檢測 40
4-5用AzA處理WJSC細胞存活率檢測 40
4-6用AzA處理WJSC流式細胞儀分析 41
4-7用AzA處理WJSC後轉染siCXCR4細胞存活率檢測 41
4-8用AzA處理WJSC後轉染siCXCR4西方墨點法檢測 42
4-9用AzA處理WJSC的條件培養液救援老化的HaCaT細胞存活率檢測 43
4-10用AzA和siCXCR4處理WJSC的條件培養液救援老化的HaCaT西方墨點法檢測 43
4-11 UVB誘導小鼠皮膚老化後用AzA處理 WJSC的條件培養液救援老化小鼠皮膚實驗設計 44
4-12 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚外觀改善情況 45
4-13 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚毛囊數目改善情況 45
4-14 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚表皮層增厚情況 46
4-15 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚真皮層的膠原蛋白流失情況 46
4-16 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚膠原蛋白標記Collagen 1 A1表現程度 47
4-17 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚老化標記p53表現程度 48
4-18 UVB誘導小鼠皮膚老化後用AzA處理WJSC的條件培養液救援老化小鼠皮膚老化標記p21表現程度 49

5.討論 50

6.結論 53

7.未來工作 54
7-1 AzA-WJSC-CM的可專利性 54
7-2是否能將AzA-WJSC-CM製成商業配方 54
7-3除了治療衰老,AzA-WJSC-CM是否可以治愈其他皮膚疾病 55
7-4探索幹細胞旁分泌蛋白途徑。 55

8.參考文獻 56

9.圖與表格 62
1.Desmond J. Tobin., Biochemistry of human skin-our brain on the outside. Chemical Society Reviews. 2006,35 ,52-67
2.MG Kosmadaki., B.A Gilchrest., The role of telomeres in skin aging/photoaging. Micron. 2004,35(3) ,155-9
3.Shreya Shanbhag., Akshatha Nayak., Reema Narayan., Usha Yogendra Nayak., Anti-aging and Sunscreens: Paradigm Shift in Cosmetics. Adv Pharm Bull. 2019,9(3) ,348-359
4.Wesuk Kang., Dabin Choi., Taesun Park., Dietary Suberic Acid Protects Against UVB-Induced Skin Photoaging in Hairless Mice. Nutrients. 2019,11(12) ,2948
5.Di Zhang., Chengtao Lu., Zhe Yu., Xiayin Wang., Li Yan., Juanli Zhang., 1 Hua Li., Jianbo Wang., Aidong Wen., Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis. Oxidative Medicine and Cellular Longevity. 2017,3 ,1-15
6.Yongxiang Liu., Ming Wangc., Xin Liu., Jia Quan., Yuan Fang., Yujie Liu., Yang Qiu., Yang Yu., Xi Zhou., Drosophila Trf4-1 involves in mRNA and primary miRNA transcription. Biochem Biophys Res Commun. 2019,511(4) ,806-812
7.Ayse Elif Erson-Bensan., Introduction to MicroRNAs in Biological Systems. Methods in Molecular Biology. 2014,1107 ,1-14
8.Mara Manciniet., Gaelle Saintigny., Christian Mahé., Margherita Annicchiarico-Petruzzelli., Gerry Melino., Eleonora Candi1., MicroRNA-152 and -181a participate in human dermal fibroblasts senescence acting on cell adhesion and remodeling of the extra-cellular matrix. Aging (Albany NY). 2012,11 ,843-53
9.Daniel Rudman, M.D., Daniel Rudman., Axel G. Feller., Hoskote S. Nagraj., Gregory A. Gergans., Pardee Y. Lalitha., Allen F. Goldberg., Robert A. Schlenker., Lester Cohn., Inge W. Rudman., Dale E. Mattson., Effects of Human Growth Hormone in Men over 60 Years Old. The New England Journal Of Medicine. 1990,323 ,1-6
10.Nikolaos Samaras., Maria-Aikaterini Papadopoulou., Dimitrios Samaras., Filippo Ongaro., Off-label use of hormones as an antiaging strategy: a review. Clinical Interventions in Aging. 2014,9 ,1175–1186.
11.Ki-Bae Hong., Yooheon Park., Jae Hwan Kim., Jin Man Kim., Hyung Joo Suh1., Effects of Porcine Placenta Extract Ingestion on Ultraviolet B-induced Skin Damage in Hairless Mice. Korean Journal for Food Science Animal Resources. 2015,35(3) ,413-420
12.Mingwu Deng., Yuda Xu., Ziyou Yu., Xiangsheng Wang., Yizuo Cai., Hongjie Zheng., Wei Li., Wenjie Zhang., Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity v. 2019,2019 ,1-11
13.Maheva Andriatsilavo., Marine Stefanutti., Katarzyna Siudeja., Carolina N. Perdigoto., Benjamin Boumard., Louis Gervais., Alexandre Gillet-Markowska., Lara Al Zouabi., François Schweisguth., Allison J. Bardin., Spen limits intestinal stem cell self-renewal. PLoS Genetics. 2018,14(11) ,e1007773
14.Aligholi Sobhani., Neda Khanlarkhani., Maryam Baazm., Farzaneh Mohammadzadeh., Atefeh Najafi., Shayesteh Mehdinejadiani., Fereydoon Sargolzaei Aval., Multipotent Stem Cell and Current Application. Acta Medica Iranica. 2017,55(1) ,6-23
15.Gina D. Kusuma., James Carthew., Rebecca Lim., Jessica E Frith., Effect of the Microenvironment on Mesenchymal Stem Cell Paracrine Signaling: Opportunities to Engineer the Therapeutic Effect. Stem Cells and Development. 2017,26(9) ,617-631
16.Huirong Li., Ling Hou., Regulation of melanocyte stem cell behavior by the niche microenvironment. Pigment Cell Melanoma Research. 2018,31(5) ,556-569
17.Zhonghao Tao., Shihua Tan., Wen Chen., Xin Chen., Stem Cell Homing: a Potential Therapeutic Strategy Unproven for Treatment of Myocardial Injury. Journal of Cardiovascular Translational Research. 2018,11(5) ,403-411
18.Shu Guo., Ting Wang., Shuangyi Zhang., Peng Chen., Zheng Cao., Wenqin Lian., Jiayan Guo., Yue Kang., Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages. Molecular and Cellular Biochemistry. 2020,463(1-2) ,67-78
19.Chien-Huang Wu., Jen-Shin Song., Hsuan-Hao Kuan., Szu-Huei Wu., Ming-Chen Chou., Jiing-Jyh Jan., Lun K Tsou., Yi-Yu Ke., Chiung-Tong Chen., Kai-Chia Yeh., Sing-Yi Wang., Teng-Kuang Yeh., Chen-Tso Tseng., Chen-Lung Huang., Mine-Hsine Wu., Po-Chu Kuo., Chia-Jui Lee., Kak-Shan Shia., Development of Stem-Cell-Mobilizing Agents Targeting CXCR4 Receptor for Peripheral Blood Stem Cell Transplantation and Beyond. Journal of Medicinal Chemistry. 2018,61(3) ,818-833
20.Laurie A.Boyer., Tong Ihn Lee., Megan F Cole., Sarah E Johnstone., Stuart S Levine., Jacob P Zucker., Matthew G Guenther., Roshan M Kumar., Heather L Murray., Richard G Jenner., David K Gifford, Douglas A Melton., Rudolf Jaenisch., Richard A Young., Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005,122(6) ,947–956.
21.Cristina Olgasi., Maria Talmon., Simone Merlin., Alessia Cucci., Yvonne Richaud-Patin., Gabriella Ranaldo., Donato Colangelo., Federica Di Scipio., Giovanni N. Berta., Chiara Borsotti., Federica Valeri., Francesco Faraldi., Maria Prat., Maria Messina., Piercarla Schinco., Angelo Lombardo., Angel Raya., Antonia Follenzi1., Patient-Specific iPSC-Derived Endothelial Cells Provide Long-Term Phenotypic Correction of Hemophilia A.Stem Cell Reports. 2018, 11(6) ,1391-1406
22.Zehong Chen., Xiaoyan Han., Xi Ouyang., Jiafeng Fang., Xuna Huang., Hongbo Wei., Transplantation of induced pluripotent stem cell-derived mesenchymal stem cells improved erectile dysfunction induced by cavernous nerve injury. Theranostics. 2019,9(22) ,6354-6368
23.Gwang-Bum Im., Yeong Hwan Kim., Yu-Jin Kim., Sung-Won Kim., Euiyoung Jung., Gun-Jae Jeong., Ke Wang., Jinheung Kim., Dong-Ik Kim., Tae-Hyung Kim., Gi-Ra Yi., Taekyung Yu., Suk Ho Bhang., Enhancing the Wound Healing Effect of Conditioned Medium Collected from Mesenchymal Stem Cells with High Passage Number Using Bioreducible Nanoparticles. Molecular sciences. 2019,20(19) , 4835
24.Tung Sheng Chen., Da-Tong Ju., Cecilia-Hsuan Day., Yu-Lan Yeh., Ray-Jade Chen., Vijaya Padma Viswanadha., Ruey-Lin Chang., Yuan-Chuan Lin., Chun-Hsu Yao., Chih-Yang Huang., Protective effect of autologous transplantation of resveratrol preconditioned adipose-derived stem cells in the treatment of diabetic liver dysfunction in rat model. Journal of Tissue Engineering and Regenerative Medicine. 2019,13(9) ,1629-1640
25.Andreas Matthäus Bader., Kristin Klose., Karen Bieback., Dirk Korinth., Maria Schneider., Martina Seifert., Yeong-Hoon Choi., Andreas Kurtz., Volkmar Falk., Christof Stamm., Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro. PLoS One. 2015,10(9) ,e0138477.
26.Tung‐Sheng Chen., Da-Tong Ju., Cecilia-Hsuan Day., Yu-Lan Yeh., Ray-Jade Chen., Vijaya Padma Viswanadha., Ruey-Lin Chang., Yuan-Chuan Lin., Chun-Hsu Yao., Chih-Yang Huang., Protective effect of autologous transplantation of resveratrol preconditioned adipose-derived stem cells in the treatment of diabetic liver dysfunction in rat model. Jurnal of Cellular Physiology. 2019,13(9) ,1629-1640
27.Nurshafira Khairudin., Mahiran Basri., Hamid Reza Fard Masoumi., Shazwani Samson., Siti Efliza Ashari., Enhancing the Bioconversion of Azelaic Acid to Its Derivatives by Response Surface Methodology. Molecules. 2018,23(2) ,397
28.MarcellaNazzaro-Porro., Siro Passi., Giuseppe Zina., Aidon S. Breathnach., The Depigmenting Effect of Azelaic Acid. Journal of the American Academy of Dermatology. 1990,126(12) ,1649-1650
29.Zhang Dongdong., Yanxia Jin., Tian Yang., Qian Yang., Balu Wu., Yanling Chen., Ziyi Luo., Li Liang., Yunjiao Liu., Anjie Xu., Xiqin Tong., Can Can., Lu Ding., Honglei Tu., Yuxin Tan., Hongqiang Jiang., Xiaoyan Liu., Hui Shen., Li Liu., Yunbao Pan., Yongchang Wei., Fuling Zhou., Antiproliferative and Immunoregulatory Effects of Azelaic Acid Against Acute Myeloid Leukemia via the Activation of Notch Signaling Pathway. Frontiers in Pharmacology. 2019,10 ,1396
30.Arianna Mastrofrancesco., Monica Ottaviani., Nicaela Aspite., Giorgia Cardinali., Enzo Izzo., Klaus Graupe., Christos C Zouboulis, Emanuela Camera., Mauro Picardo., Azelaic acid modulates the inflammatory response in normal human keratinocytes through PPARgamma activation. Experimental Dermatology. 2010,19(9) ,813-20
31.Stefania Briganti., Enrica Flori., Arianna Mastrofrancesco., Daniela Kovacs., Matteo Ludovici., Giorgia Cardinali., Mauro Picardo., Azelaic acid reduced senescence‐like phenotype in photo‐irradiated human dermal fibroblasts: possible implication of PPARγ. Experimental Dermatology , 2013,22(1) ,41-7
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