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研究生:凌銘暘
研究生(外文):Ling, Ming-Yang
論文名稱:短期高糖合併發炎微環境前處理促使間葉幹細胞活化並強化糖尿病性肝損傷的治療
論文名稱(外文):Short-term hyperglycemic coupling with inflammatory microenvironment preconditioning increases mesenchymal stem cell ability in the treatment of diabetic hepatopathy
指導教授:陳冬生陳冬生引用關係
指導教授(外文):Chen, Tung-Sheng
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:生技醫藥產業碩士學位學程
學門:醫藥衛生學門
學類:藥學學類
論文出版年:2020
畢業學年度:109
語文別:中文
論文頁數:37
中文關鍵詞:糖尿病肝損傷間葉幹細胞發炎微環境
外文關鍵詞:diabetes mellitusliver injurymesenchymal stem cellsinflammatory microenvironment
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謝誌 ii
中文摘要 iv
英文摘要 v
目錄 vi
圖與表格 ix

第壹章. 緒論 1
第一節. 糖尿病現況 1
第二節. 糖尿病與併發症 1
第三節. 糖尿病與非酒精性脂肪肝 1
第四節. 小分子核糖核酸與發炎機制 2
第五節. 糖尿病性肝損傷現行治療方式 4
第六節. 幹細胞治療糖尿病性肝損傷 5
第七節. 微環境與幹細胞能力 6
第八節. 高糖合併發炎微環境促使間葉幹細胞活化 7

第貳章. 研究的重要性與目的 8

第參章. 研究材料與方法 9
第一節. 研究材料來源 9
第二節. 模擬糖尿病性肝損傷 9
第三節. 發炎微環境強化幹細胞 10
第四節. 細胞共培養技術 10
第五節. Micro RNA(miRNA)短暫轉殖 10
第六節. 共培養技術-嵌入式共培養 11
第七節. 細胞轉染小分子干擾核糖酸 11
第八節. 流式細胞儀分析(Flow Cytometry) 11
第九節. 細胞存活率檢測-MTT分析法 11
第十節. 實驗動物來源與飼養環境 12
第十一節. 實驗動物分組與誘發糖尿病 12
第十二節. 實驗動物分組與幹細胞治療 12
第十三節. 病理組織化學染色IHC 13
第十四節. 硫代巴比妥酸反應測試 13
第十五節. 西方墨點法 14
第十六節. 統計分析 14

第肆章. 實驗結果 15
第一節. 高糖環境誘導肝臟細胞損傷細胞存活率檢測 15
第二節. 高糖環境誘導肝臟細胞損傷蛋白質表現量檢測 15
第三節. 高糖環境誘導肝臟細胞損傷氧化壓力檢測 15
第四節. miRNA-3580-5p 短暫轉殖後西方墨點法檢測 15
第五節. 高糖合併發炎微環境預處理WJSCs細胞存活率檢測 16
第六節. WJSCs活化後共培養Clone 9 cells cells細胞存活率 16
第七節. 高糖合併發炎微環境預處理WJSCs流式細胞儀分析 16
第八節. 轉染不同濃度si-c-kit細胞存活率檢測和西方墨點法測試 16
第九節. WJSCs活化後後轉染si-c-kit細胞存活率檢測 17
第十節. WJSCs活化後後轉染si-c-kit幹細胞共培養治療受損肝臟細胞細胞存活率檢測 17
第十一節. WJSCs活化後後轉染si-c-kit幹細胞共培養治療受損肝臟組織西方墨點法測試 17
第十二節. 實驗動物誘發糖尿病後血糖變化 17
第十三節. 實驗動物體重變化 17
第十四節. 動物實驗生化指標 18
第十五節. 肝臟組織切片染色 18
第十六節. 肝臟組織化學染色IHC 18
第十七節. 肝臟組織西方墨點法測試 19

第伍章. 討論 20
第一節. 高糖環境誘導肝臟細胞損傷 20
第二節. miRNA-3580-5p 短暫轉殖 21
第三節. 高糖合併發炎微環境預處理活化WJSCs 22
第四節. 高糖合併發炎微環境預處理活化WJSCs流式細胞儀分析 22
第五節. 轉染不同濃度si-c-kit細胞存活率檢測和西方墨點法測試 22
第六節. WJSCs活化後後轉染si-c-kit 23
第七節. WJSCs活化後通過共培養治療Clone 9 cells cells 23
第八節. 實驗動物生理和生化指標 24
第九節. 肝臟組織 24

第陸章. 結論 25

參考文獻 26
附錄 31
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