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研究生:葉佳文
研究生(外文):Yeh, Chia-Wen
論文名稱:養肝丸(神立清)之抗氧化及肝臟保護藥效評估
論文名稱(外文):Efficacy Assessment of Yang-Gan-Wan(Shen-Li-Qing) in Anti-oxidation and Liver Protection
指導教授:吳忠信吳忠信引用關係
指導教授(外文):Wu, Chung-Hsin
學位類別:碩士
校院名稱:國立臺灣師範大學
系所名稱:生技醫藥產業碩士學位學程
學門:醫藥衛生學門
學類:藥學學類
論文出版年:2021
畢業學年度:109
語文別:中文
論文頁數:65
中文關鍵詞:中藥養肝丸抗氧化自由基肝臟保護
外文關鍵詞:Traditional Chinese MedicineYang Gan WanAnti-oxidant free radicalsLiver protection
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中文摘要
在臺灣,民眾罹患肝病盛行率居高不下,而肝病造成之死亡率,多年來亦高居十大死因之前幾位,因此開發有效保護肝臟功能的藥物,一直是生技醫藥產業積極投注資金及研發的重點項目。過去西方醫學對於肝病的治療多採取支持性的保守療法,對於具體促進肝功能的西藥開發進程更是緩慢。從中醫學古籍中草藥相關之記載中,對於保護肝臟的中藥方已有相當清楚的認識,惟肝病中醫藥雖然源遠流長數千年,但由於西方科學盛起之故,未經科學研究證實功效的中醫藥反而屢受質疑。為此,本論文主要研發具保護肝臟功能的傳統中藥,以科學實驗的研究證實相關藥物功效。本研究選用順天堂中藥養肝丸(神立清)顆粒劑探討其抗氧化及肝臟保護的藥效。利用清除DPPH自由基能力測定實驗,本實驗研究結果發現養肝丸(神立清)顆粒劑具備顯著的清除DPPH自由基能力;利用活體外細胞實驗,結果發現養肝丸(神立清)顆粒劑可以緩解因革蘭陰性菌外膜成分脂多糖(LPS)誘發肝臟細胞的損傷。此外,利用動物餵食實驗,結果發現養肝丸(神立清)顆粒劑可以保護小鼠對乙醯胺酚(APAP)誘發的肝毒性。綜觀順天堂中藥養肝丸(神立清)顆粒劑,效能確實並具有抗氧化的功效,再者目前經由科學學理實驗研究證實養肝丸(神立清)顆粒劑確實具有保護肝臟機能功效,因此應該可以作為保肝的中藥。
Abstract
In Taiwan, the prevalence rate of liver disease among the people remains high, and the death rate caused by liver disease has been the highest among the top ten causes of death for many years. Therefore, the development of drugs that effectively protect liver function has always been an active investment and key projects for R&D of the biotechnology and pharmaceutical industry. In the past, Western medicine used supportive conservative treatments for liver disease, and the development of western medicines that specifically promote liver function was slow. From ancient records of Chinese herbal medicines, there is a clear understanding of traditional chinese medicines (TCM) for protecting the liver functions. However, although TCM for liver disease has a long history of thousands of years, due to the rise of Western science, TCM that have not been proven by scientific research, thus TCM have been challenged. In this study, a scientific Chinese medicine of Sun Ten Pharmaceutical Co., Ltd. " Yang Gan Wan (Shen Li Qing) " (YGW) granules were used to study its anti-oxidation and liver protection effects. By using the DPPH free radical scavenging ability measurement experiment, our results found that YGW granules have significant DPPH free radical scavenging ability. By using cell experiments in vitro, our results found that YGW granules could relieve lipopolysaccharide (LPS) -induced damage in liver cells. In addition, by using animal feeding experiments, our results found that YGW granules can protect mice from liver toxicity induced by Acetaminophen (APAP).To summary the function of YGW granules that have anti-oxidant effects and can indeed protect liver function. We suggested that YGW granules could be an ideal TCM for liver protection.
第一章 緒論 1
第一節 研究背景與動機 1
第二節 文明疾病引發氧化壓力 3
第三節 氧化壓力誘發肝臟損傷 5
第四節 中醫藥學理論與肝臟的生理病理聯繫 9
第五節 中醫藥學理論與肝臟保護有關的中藥 12

第二章 研究目的與設計 18
第一節 研究目的 18
第二節 實驗設計 18

第三章 材料與方法 19
第一節 順天堂中藥養肝丸(神立清)顆粒劑成份分析 19
第二節 清除1,1-二苯基-2-三硝基苯肼(DPPH)氧化自由基能力測定 19
第三節 脂多醣(LPS)誘發肝臟巨噬細胞發炎模式 20
第四節 乙醯胺酚(APAP)誘發肝損傷動物模式 23
第五節 血清生化值分析 24
第六節 肝臟組織染色 24
第七節 統計與資料分析 26

第四章 研究結果 27
第一節 養肝丸(神立清)顆粒劑的中藥指紋圖譜 27
第二節 養肝丸(神立清)顆粒劑抗氧化、清除自由基的能力 27
第三節 養肝丸(神立清)顆粒劑對肝臟巨噬細胞損傷的保護作用 28
第四節 養肝丸(神立清)顆粒劑對小鼠肝損傷的保護作用 29

第五章 討論與結論 32
第一節 養肝丸(神立清)顆粒劑的抗氧化作用 32
第二節 養肝丸(神立清)顆粒劑對肝細胞損傷的保護作用 32
第三節 養肝丸(神立清)顆粒劑對動物誘發肝損傷的保護作用 34
第四節 結論 37

參考文獻 38
附錄圖表 45
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