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研究生:陳怡如
研究生(外文):CHEN, YI-JU
論文名稱:探討丹參酮IIA對小鼠星狀細胞受到廣東住血線蟲第五期幼蟲排泄分泌產物處理後之治療效果
論文名稱(外文):Studies on the therapeutic effect of Tanshinone IIA in astrocytes after Angiostrongylus cantonensis fifth stage larval excretory-secretory products treatment
指導教授:陳光耀陳光耀引用關係
指導教授(外文):CHEN, KUANG-YAO
口試委員:林雅玲鄭尉弘
口試委員(外文):LIN, YA-LINGCHENG, WEI-HUNG
口試日期:2022-07-08
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:生物醫學研究所碩士班
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2022
畢業學年度:110
語文別:中文
論文頁數:49
中文關鍵詞:廣東住血線蟲排泄分泌產物星狀細胞丹參酮IIA(TSIIA)
外文關鍵詞:Angiostrongylus cantonensisExcretory-secretory products (ESPs)AstrocytesTanshinone IIA (TSIIA)
IG URL:yiruchen830318
Facebook:陳怡如 (Judy Chen)
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廣東住血線蟲是一種寄生性線蟲,可引起人類廣東住血線蟲症,臨床上在東南亞和太平洋島嶼常造成嗜酸性腦膜炎,嚴重時會導致死亡。排泄分泌產物(ESPs)是研究寄生性蠕蟲與宿主之間相互作用的重要標的,其中包含許多分子被認為是寄生蟲發育、攝食、入侵和蛻皮的關鍵因素。丹參酮IIA (TSIIA) 是一種血管活性心臟保護藥物,被廣泛用於評估潛在治療機制的研究,包括單核細胞粘附、巨噬細胞膽固醇積累和發炎細胞因子之表達。本研究擬釐清丹參酮IIA的分子機轉並評估受到廣東住血線蟲排泄分泌產物刺激下對於小鼠星狀細胞之治療結果。首先,研究結果星狀細胞在排泄分泌產物刺激後,丹參酮IIA可以提高細胞活力來來達到治療效果。另一方面,在丹參酮IIA治療中,細胞凋亡相關分子的表現量有明顯下降的趨勢。然而,抗氧化壓力、自噬作用和內質網壓力等相關分子的表現量則是顯著性上升。最後,結果顯示丹參酮IIA也能刺激超氧化物歧化酶(SOD)、穀胱甘肽 S-轉移酶(GST)和過氧化氫酶(Catalase)等抗氧化相關蛋白的活化並抑制排泄分泌產物所引起的 ROS 生成。此研究結果證實星狀細胞在廣東住血線蟲排泄分泌產物刺激下,丹參酮IIA確實能降低其所引起的損傷或死亡,並釐清相關分子機轉。
Angiostrongylus cantonensis is a parasitic nematode that causes human angiostrongyliasis, such as eosinophilic meningitis in Southeast Asia and the Pacific Basin. Excretory-secretory products (ESPs) is the important factor for studying the interaction between parasitic helminths and hosts. It has been known as a key factor for parasite development, feeding, invasion and molting. Tanshinone IIA (TSIIA), the vasoactive cardioprotective drug, is used in a wide variety of studies to assess the potential therapeutic mechanism, including monocyte adhesion, macrophage cholesterol accumulation, and proinflammatory cytokine expression. This study was designed to investigate the molecular mechanisms of TSIIA and to evaluate the therapeutic consequent in astrocytes after A. cantonensis ESPs. First, our results found that TSIIA can protect the astrocytes via increasing the cell viability after ESPs treatment. Furthermore, the expression of cell apoptosis related molecules were significantly reduced in TSIIA treatment. However, the expression of oxidative stress, autophagy, and ER stress related molecules were significantly increased. Finally, the results showed that TSIIA can inhibit the ESPs-induced ROS generation through stimulation of the antioxidant activation, such as Superoxide dismutase, Glutathione S-transferase, and Catalase. Collectively, these results demonstrate that TSIIA has the astrocytes protective function after A. cantonensis ESPs treatment.
誌謝辭 I
中文摘要 II
英文摘要 III
目錄 IV
圖目錄 V
一、前言 1
二、實驗材料與方法 10
三、結果 16
四、討論 19
五、結論 22
六、參考文獻 23
七、圖表 29
八、附錄(試劑列表) 46
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