臺灣博碩士論文加值系統

製備層析法之中的收集是一項重要的程序，然而在微流體裝置上，層析系統通常被用於分析層析法上，因此多數的研究則不進行洗脫液的收集程序，在我們先前的研究之中，我們在離心平台上建立了製備層析法的基礎，為了改善洗脫液收集的結果，以提高各組分的純度，因此本研究著重於開發離心平台上的分液收集器，將洗脫液分成數個獨立的組分，藉由科氏力作用，可以將洗脫液送至不同的收集槽位置，藉此以達到分液收集器的效果。

A few fluidic designs for fraction collectors of chromatography on a centrifugal platform were developed. In our previous effort, the preparative chromatography on a centrifugal platform was established. In order to improve the purity of the eluate, an effective fraction collector is of great importance. In this work, we proposed a few fraction collector designs using flow switching techniques. By changing the rotational speed, the Coriolis-induced flow would direct the eluate into the corresponding collecting chambers. The experimental results showed that water-soluble dyes can be successfully separated and recovered by the proposed fraction collectors.

圖目錄 ........................................................................................................................ 6
表目錄 ... 9
第一章 緒論 10
1.1 前言................................................................................................................... 10
1.2 離心式微流體技術介紹 11
1.3 層析法 13
1.4 分液收集器 15
1.5 研究動機 16
第二章 文獻回顧 17
2.1 離心式微流體單元介紹 17
2.2 微流體上之層析應用 22
2.3 離心旋轉式層析發展 26
2.4 微流體裝置上之洗脫物收集方法 30
2.5 離心平台上之流動切換應用 33
第三章 實驗設計 36
3.1 實驗藥品與材料 36
3.1.1 層析之水溶性色素 36
3.1.2 層析之分析藥品 36
3.1.3 研究使用之材料 37
3.2 實驗儀器 38
3.3 離心式層析操作平台 39
3.3.1 馬達儀控系統 39
3.3.2 影像擷取系統 40
3.3.3 偵測系統 42
3.4 碟片設計與製作 43
3.4.1 碟片設計及製作 43
3.4.2 離心式微流體操作流程 46
3.5 實驗分析 49
3.5.1 灰階分析法 49
3.5.2 分光光度計分析 49
第四章 實驗結果 51
4.1 離心式微流體層析之分離探討 51
4.1.1 轉速對分離的影響 51
4.1.2 固定相粒徑對分離之影響 54
4.2 離心平台上收集之因素探討 58
4.2.1 不同轉速下的擴散情形探討 58
4.2.2 溶液組成對擴散之影響 61
4.2 流動切換之收集方法 63
4.3 離心式分液收集器 69
4.3.1 碟片設計及封裝 69
4.3.2 偏移之起始半徑位置 71
4.3.3 不同組分之溶劑與偏移角度關係 74
4.3.4 離心式分液收集器之應用 76
第五章 結論 77
第六章 未來展望 78
第七章 參考文獻 79

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