臺灣博碩士論文加值系統

摘要 i
Abstract ii
目次 iv
表目次 vi
圖目次 vii
壹、 前言 1
貳、 文獻探討 2
一、 熱緊迫 2
(一) 熱緊迫之定義 2
(二) 雞隻體溫調節 4
二、 下視丘與熱緊迫之調節 5
(一) 下視丘之位置、結構與功能 5
(二) 下視丘與體溫調節 8
(三) 下視丘與食慾調節 9
三、 熱緊迫對雞隻之影響 10
(一) 熱緊迫對雞隻之採食及生長之影響 10
(二) 熱緊迫對雞隻內分泌之影響 11
(三) 熱緊迫對雞隻代謝之影響 13
四、 Vitamin D 之代謝 15
五、 維生素D的傳統生理功能 17
(一) 維生素D對於骨骼形成的調節 17
(二) 維生素D對於骨骼吸收的影響 18
(三) 維生素D對於腸道的鈣吸收 19
六、 維生素D3與雞隻生長及免疫之關係 20
(一) 25-OH-D3對於雞隻生長及免疫之影響 20
(二) 25-OH-D3對於雞隻免疫及發炎之影響 20
七、 研究目的 21
參、 材料與方法 22
一、 實驗動物與飼養設計 22
(一) 動物來源與分組 22
(二) 熱緊迫處理 22
(三) 生長性狀紀錄 22
(四) 樣品採集 23
二、 維生素D來源與添加 23
三、 基礎生理指標之檢查 23
(一) 直腸溫度之紀錄 23
(二) 呼吸次數之測定 23
四、 血液生化分析 24
(一) 血液pH值測定 24
(二) 血漿中Sphingomyelin測定 24
(三) 血漿中內分泌之測定 24
五、 雞隻下視丘mRNA樣品製備與分析 27
(一) 組織mRNA萃取 27
(二) mRNA完整度測試 28
(三) cDNA之製備 28
(四) 引子設計及驗證 29
(五) 即時聚合酶連鎖反應 31
六、 統計分析 32
肆、 結果 33
一、 ROSS308白肉雞生長性狀 33
二、 AA白肉雞生長性狀 36
三、 ROSS308白肉雞直腸溫度 38
四、 ROSS308白肉雞呼吸速率 38
五、 ROSS308白肉雞血液pH值 41
六、 ROSS308白肉雞血漿中內分泌之分析 43
(一) 血漿中ACTH含量 43
(二) 血漿中corticosterone含量 44
(三) 血漿中T3與T4的含量 44
(四) 血漿中腎上腺素含量 47
(五) 血漿中sphingomyelin含量 47
七、 ROSS308白肉雞下視丘食慾相關基因表達量 50
伍、 討論 53
一、 不同形式維生素D對熱緊迫下白肉雞生長性狀之影響 53
二、 不同形式維生素D對熱緊迫下白肉雞體溫、呼吸與血液pH值之影響 54
三、 不同形式維生素D對熱緊迫下白肉雞內分泌之影響 55
四、 不同形式維生素D對熱緊迫下白肉雞食慾相關基因之調節 56
陸、 結論 57
柒、 參考文獻 58

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